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The map label for this gene is dotB

Identifier: 52842882

GI number: 52842882

Start: 3022673

End: 3023836

Strand: Direct

Name: dotB

Synonym: lpg2676

Alternate gene names: 52842882

Gene position: 3022673-3023836 (Clockwise)

Preceding gene: 52842881

Following gene: 52842883

Centisome position: 88.96

GC content: 42.27

Gene sequence:

>1164_bases
CTGGCAACCTATTATAGCACCAGTAAGTTAATGTCTATGGATAATATTAATTTAATGCCCGATGAGCCAACTCGTTTCAC
TCCAGTATTCATGGACAGAATGCTGGAGCATGCAGAAAGCCTGAATGCTTCAGATATAACAATCCAAACTGGCGAACCCA
TTTTTGCAGAGGTTTATGGAAGACTCCTTAAAATTACAAATCGACGTCTTTCCAATACAGAATTGGGCGACCTTATTAAT
TCCATTTATGGCCCGAATGCCACGACACAATTACTTTCCGGTAAAGATATAGATACTCATTATGAGTTTCGACCCAACCG
TGGTGTACGCTATCGATATAGGGTTAACGCGACTGCCTGCCTGGTTGAAGGTCATGATGCCATTCAAATCACATTAAGAA
CTATTCCAACCACGCCACCCAAACTCAGTACCATGAACCTCCCTGATAACATTATTGAAGCGATTGCTCCACAGGAAGGA
ATCGTGTTTATTACAGGCGCTACCGGTTCGGGCAAATCGACATTGTTAGCTTCCATTATTCGAGAACTTATTGAAACATC
AGACTCAAACCGTAAAGTATTAACTTACGAATCCCCGATAGAATTCGTCTATGATGAAATTGAAACAATTTCCGCTGTAG
TAAGCCAATCAGAGATTCCAAGACACTTACCCAACTTTGCTGATGGAGTAAGAAACGCATTAAGACGAAAGCCAAGATTA
ATTATGGTGGGTGAATGTCGTGATGCAGAAACCATCAGTGCCGCTCTTGAAGCCGCTTTAACAGGGCACCCTGTCTACAC
AACCCTGCATACTTCTGGTGTTGCAGAAACAATGCGACGTCTGGTCACATCTTTTAGCGGGGAAGAGCGTTTGGGAAGAA
CCATCGATATTCTTGAAACAATTAGGCTTTGCATTTGGCAAAAACTGGTCCCAACCGTTGATGAAAGACGTGTAGCCCTA
AGGGAGTATCTGGTATTCGATGAAGAAGTGAGAGATATTCTATTGGAGGGAGACCCTAACGAAGTGACTTCAGCCACTCG
TAAGTTAGTGAGGCAAAAAGGGCAGTTAATGACTTGGGACGCAAAAATGAAATTTGAACAGGGGATTATCAGTGAGCGAG
TTTATAAATTAATCATTGCTGGGGCCAAAGAATATCAACAATAA

Upstream 100 bases:

>100_bases
AATGGAGAGCTGCGGTCGCCAAAGATGAAAATGCTCTCGAACGTTTCAAAAATATGGAAAAACTGGCAAATCAGGCTAAA
ATTGTTATAACAAACAAATC

Downstream 100 bases:

>100_bases
TCAAAATTAATCTGTTTGCACTTGCCATAGAAAAATCATTGATATACCGCATATAAAACAATCAAGCTCAAGGATTCGAT
TCATGATGTTTCACAGGAAA

Product: defect in organelle trafficking protein DotB (ATPase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MATYYSTSKLMSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLIN
SIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEG
IVFITGATGSGKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRL
IMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVAL
REYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Sequences:

>Translated_387_residues
MATYYSTSKLMSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLIN
SIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEG
IVFITGATGSGKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRL
IMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVAL
REYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ
>Mature_386_residues
ATYYSTSKLMSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINS
IYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGI
VFITGATGSGKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLI
MVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALR
EYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=216, Percent_Identity=38.4259259259259, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789723, Length=150, Percent_Identity=39.3333333333333, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786296, Length=133, Percent_Identity=39.0977443609023, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 43603; Mature: 43471

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYYSTSKLMSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYG
CCCEECHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATAC
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEE
LVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASII
EEECCCEEEEEEEECCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
RELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRL
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
IMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILET
EEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHH
IRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEH
AKMKFEQGIISERVYKLIIAGAKEYQQ
HHHHHHHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
ATYYSTSKLMSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYG
CCEECHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATAC
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEE
LVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASII
EEECCCEEEEEEEECCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
RELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRL
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
IMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILET
EEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHH
IRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEH
AKMKFEQGIISERVYKLIIAGAKEYQQ
HHHHHHHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]