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The map label for this gene is rsmD [H]

Identifier: 52842879

GI number: 52842879

Start: 3020700

End: 3021245

Strand: Direct

Name: rsmD [H]

Synonym: lpg2673

Alternate gene names: 52842879

Gene position: 3020700-3021245 (Clockwise)

Preceding gene: 52842878

Following gene: 52842880

Centisome position: 88.9

GC content: 38.28

Gene sequence:

>546_bases
TTGAAACAGGTAATAAGGATTATTGGAGGATTATATCGGGGGAAAAAAATACATTTCCCCGATGTAGATGGCCTGAGGCC
AACACCAGACAGAGTTCGGGAGACCCTCTTTAATTGGTTAATGAATGATATTAAAAATGCCCGCTGTCTGGATGCTTTTG
CAGGTAGTGGCGCGTTGGGATTTGAAGCCTATTCCAGAGGAGCGTCTCATGTCGTTTTTATCGAACAATCTCCCAAAGCT
CATGCCAGTTTGCAAAATACCATTCGTCAATTTGATACCCCCAAATTGCACCTTATAAAAACTGATACGTTAAATTATCT
TAAACAAAGCACCGAGCAATTCGATCTGATTTTTTTAGATCCTCCCTACGCTCTCAACTATGTTCCTCAGTGTCTTGATC
ATATCATAACAAATAACCTTCTTGTTAAAGGAGGTCTTGTCTACATTGAATCATCATCTCCTATTGAAGTAAAAACAGAA
CTTTGGAAGCAATTGAAATTAAAACAAGCCGGTCAAGTCATTTATGGATTATTTGAAAAACTCTAA

Upstream 100 bases:

>100_bases
ATGTTGCTAAAATCAATGCGGTAACCGATGCTGAAATCAAACAAGCATTCCAGCAACAGGTGAATCCTGAAAAATTACTC
TTGGTAACGGTTGGACAATC

Downstream 100 bases:

>100_bases
CAAACAATGTCTTGACAACAAGCCTTCCTCCTGTTTCAATCAAACTAATATTTCCAAATGGTAAAGAACATTGAATGAAC
AACAATAAGATTGTCATAAT

Product: N6-adenine specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKA
HASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
LWKQLKLKQAGQVIYGLFEKL

Sequences:

>Translated_181_residues
MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKA
HASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
LWKQLKLKQAGQVIYGLFEKL
>Mature_181_residues
MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKA
HASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
LWKQLKLKQAGQVIYGLFEKL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=182, Percent_Identity=39.010989010989, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20575; Mature: 20575

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDIKNARCLDAFAGSGALG
CHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
FEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLD
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEEEEEEC
PPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTELWKQLKLKQAGQVIYGLFEK
CCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
L
C
>Mature Secondary Structure
MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDIKNARCLDAFAGSGALG
CHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
FEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLD
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEEEEEEC
PPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTELWKQLKLKQAGQVIYGLFEK
CCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]