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The map label for this gene is trmL [H]

Identifier: 52842704

GI number: 52842704

Start: 2813566

End: 2814090

Strand: Direct

Name: trmL [H]

Synonym: lpg2496

Alternate gene names: 52842704

Gene position: 2813566-2814090 (Clockwise)

Preceding gene: 52842702

Following gene: 52842706

Centisome position: 82.81

GC content: 39.24

Gene sequence:

>525_bases
TTGCATCCTGCCACTCTATTCCTGGCTTTGAATAAATCAATTAAAATGGCAGCCAGAATGTTACTGGAAAATCACATGAT
CCATATTGCGCTTTATCAACCAGAAATTCCACCGAACACGGGCAATATTATTCGACTCTGCGCCAATACTGGAGCTCAAC
TGCATTTGATTCATCCATTAGGCTTTACTTTGGAAGATAAACAAATGCGACGAGCCGGTCTTGACTATCATCAATGGGCC
TCAATATTGCATTATGAGAATTGGCAAACATTCTTGCAGTCTCATTCACAACGACGATTATTTTGCTGTAGCGCAAAAGG
AAAAGTGAAGTATAGTGACGTCGATTATAAGGCAGATGACATCTTATTATTTGGACCCGAATCTTGCGGCTTACCTCAAC
AAATACTGAACCAATACTCAACAATACGGATTCCCATGAGAGAAAATAATCGTAGTCTCAACTTATCAAATGCTGTAGCA
ATCATATTGTACGAAGCATGGAGGCAAATGGATTTTAACATGTAA

Upstream 100 bases:

>100_bases
CATACTAATATTGTAGTAGCTATGTTGAATTCAGACAGCCTGTTGAGTAAAAAATTAGACACATCAACTCCCTGCTTGAT
TTTTAAAGTATGTAGTAAAA

Downstream 100 bases:

>100_bases
GCATTAACTTGGTAATCCACCTGATAGCGATTACAGCAATGGAAGGAACAGGTTATGCAAGCAGTACTCTAATATAAACA
GGACTTACGAAAAACCACAA

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MHPATLFLALNKSIKMAARMLLENHMIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQMRRAGLDYHQWA
SILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVA
IILYEAWRQMDFNM

Sequences:

>Translated_174_residues
MHPATLFLALNKSIKMAARMLLENHMIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQMRRAGLDYHQWA
SILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVA
IILYEAWRQMDFNM
>Mature_174_residues
MHPATLFLALNKSIKMAARMLLENHMIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQMRRAGLDYHQWA
SILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVA
IILYEAWRQMDFNM

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=52, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20129; Mature: 20129

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPATLFLALNKSIKMAARMLLENHMIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPL
CCCEEEEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECC
GFTLEDKQMRRAGLDYHQWASILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADD
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCC
ILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM
EEEECCCCCCCHHHHHCCHHEEEEEECCCCCEEECCHHHHHEEEHHHHHHCCCC
>Mature Secondary Structure
MHPATLFLALNKSIKMAARMLLENHMIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPL
CCCEEEEEEECCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECC
GFTLEDKQMRRAGLDYHQWASILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADD
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCC
ILLFGPESCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM
EEEECCCCCCCHHHHHCCHHEEEEEECCCCCEEECCHHHHHEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA