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The map label for this gene is cmlA [H]

Identifier: 52842647

GI number: 52842647

Start: 2753605

End: 2754795

Strand: Reverse

Name: cmlA [H]

Synonym: lpg2438

Alternate gene names: 52842647

Gene position: 2754795-2753605 (Counterclockwise)

Preceding gene: 52842648

Following gene: 52842634

Centisome position: 81.08

GC content: 44.33

Gene sequence:

>1191_bases
TTGAATTTTATTGGCTGGACAGAAAGACAAAGTCTTTTTCGATTATTTCCACTCATTATCAGCATTTCTTTTGCTATGGA
TGTTTTTGTGCCCGCAATTCCGGAAATGAGTCGTTATTTCAATACTGACAGTACAACGATGCAAGCTACCCTTTATTTGT
TTATGCTCACTGTAGCTTTGGGTCAGCTATTGGTTGGGCCTTTGGCCGATCGTTTTGGACGAAGACAGATGGCCTTGAGT
ACGGCACTTCTTTTTTTTGCAGGATCGTTGATCTCTGCGTTAGCTCCGTCAGTACCGGCATTGATTTTTGCACGTATCAT
TCAGGCAGCTGGTGCCTGTGGAACCTATTTACTATGCTTTATTATCGTTCGAGATAATTTTTCAACGGGCACCTGTGCCC
GCTTGTTTAGTATCCTGTCTGGGACGAATTCGATGGTTGCCAGTTCTGCTCCAGTTATTGGGGGATTATTGTTGGATATG
ACGCAAGATTGGCATAGTGGATTCTACTTTTTAACCTTGCTTGGGTTATTAATGACTGGCATAGCCTTCCGTTACATCCC
TGATTATCACTATCCCAAACCCGAGCTGCTCCAGATGAGGGTGCACCAAGTGATGAGGCAGATAATAAGTAACTCTGACT
TTCGCCAATACACATGGGTTGCCTCAGCCAGTTTGCTGGGATTGTACTTGTTTTGTGCTCAATCACCAGGTATTTTGATC
ACACAACTGTACCTGAGTGCGACTGAATATGGTTTCTGGTTTGGTTTGAATGCAATGACTGTGTTTGTTGCCAATATGAT
GGCCGCACGACTTACTTATTCTCACTCATTGGAGAGCATAGTGCGTTGGGGCTTGGTTTTAATGACACTGTCATGCTTTT
TTATGCTGGCTCTTAATCTTCGTCAATGCAGCATTTTAAGTTTTATGCTTCCCATGCTTTGCTTGACTGTGGGTATTGGT
ATTAGCATGGGCACAGCAACAGCGCTTGCTTTAAAGGATTTTGAACAGCAAGCTGGCGTCGCTACCGCTCTGTTGGGTGC
TTGCCAGTTTGGTCTCGCGGGCCTGATTGGTGTTTTGACAGCTCAATGGATGCCCGGGCCATTAAGTGTAGCTATCCCCG
TACTTTGTTTCACTGCAGTGGGATTGGCTAGAATCACCAAATTCAGTTTTAGAATATTGAGCGGCTCTTAA

Upstream 100 bases:

>100_bases
TCTGTTGAATCAGCAATTTTATAACATTGAGTGTAAATCTGTTACAATTAAGCGCAGTTTATATTTTTCATAAGTAATAT
TTTATCAGGAACACCATTAA

Downstream 100 bases:

>100_bases
TCTTGTCCTCAGGCAATTGGCCAAAAAAGACCGATTATAGGTTATGCTGTAGGTAACACTGTGACATTTAGTGTTGTTTA
TCCCGGGTGTGGTTCTGTAT

Product: florfenicol efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALS
TALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
TQDWHSGFYFLTLLGLLMTGIAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYTWVASASLLGLYLFCAQSPGILI
TQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Sequences:

>Translated_396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALS
TALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
TQDWHSGFYFLTLLGLLMTGIAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYTWVASASLLGLYLFCAQSPGILI
TQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
>Mature_396_residues
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALS
TALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
TQDWHSGFYFLTLLGLLMTGIAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYTWVASASLLGLYLFCAQSPGILI
TQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIG
ISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=342, Percent_Identity=26.0233918128655, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790146, Length=337, Percent_Identity=23.1454005934718, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082312, Length=339, Percent_Identity=25.3687315634218, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI48994889, Length=365, Percent_Identity=23.2876712328767, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43362; Mature: 43362

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
GQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMTG
HHHCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
IAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYTWVASASLLGLYLFCAQSPGILI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDSTTMQATLYLFMLTVAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
GQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALIFARIIQAAGACGTYLLCF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
IIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDMTQDWHSGFYFLTLLGLLMTG
HHHCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
IAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYTWVASASLLGLYLFCAQSPGILI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHSLESIVRWGLVLMTLSCFFMLALNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQCSILSFMLPMLCLTVGIGISMGTATALALKDFEQQAGVATALLGACQFGLAGLIGVLT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQWMPGPLSVAIPVLCFTAVGLARITKFSFRILSGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]