Click here to switch to the map view.

The map label for this gene is gatA1 [H]

Identifier: 52842565

GI number: 52842565

Start: 2659286

End: 2660695

Strand: Reverse

Name: gatA1 [H]

Synonym: lpg2355

Alternate gene names: 52842565

Gene position: 2660695-2659286 (Counterclockwise)

Preceding gene: 52842567

Following gene: 52842563

Centisome position: 78.31

GC content: 44.61

Gene sequence:

>1410_bases
ATGGATGCCATTCATCTCCTTTCTGCAACAGAAATCATTCGCCGCATTAAAATGAAAGAACTCTCGGCTGCAGAGGTCAT
GGTGGCGCACTTAAACCATATTGATAAGATTAATCCTGTAATCAATGCGCTCACCGAACGTATTCCCCCTGAGGAATGCT
TAAAGCAGGCAAAGGAAATTGACAAGAGTATCTCTTCGAAAAAAAATCTGAACAAGTTAATGGGGTTGCCTGTTGCCATA
AAAGACGCTTTGTATGTGAAAGGACTAATTTGTTCTTCGGCATGTTCAGGATTTTACAAGGGAGAAAAAGCAGTACGTGA
TGCGACCCTGGTTTCTCGACTGAAAAAGGAAGGAGCAATTATTCTTGGCTTAACTAATGTTCCTGAATTGTGTCGCGGTG
GTGATTCGGATAATTTAATCTATGGACGGACCAATAATCCTTATGATCTGGCAAGAACCAGTGGAGGCAGTAGTGGTGGT
TCCGCGGCTTTGATCGCTGCAGGAGGCGTTCCTTTTGCTTTGGGCTCCGATGGTGGAGGTAGTTTAATGCAGCCTGCCCA
TTGTTGCGGTATTGTTGCATTGAAGCCAACACATGGCCATCTTCCTCATACAGGATCTGTAGGTGGGGATTCCTATGGTC
TTATTGGCAACCTGATTTCTTTTGGACCAATGGCTCGTTCTGTCAGTGACCTTCGTTTAGGACTCTCCGTGCTTGCGGGT
TCTGACCAATATGATCCTTATACAAATCCGGTTCCTGTTATGCCTGCTGCACCACTAAAAAAATTACGTGTAGCCTATTT
TACAGAGAATGGTTTTACGCCAGTTGATGCGGAGATACAGAACGTTGTTAAATCAGCAGCCTTGGCTTTGCAGGATGATG
TGGCTATAGTGAGGGAGGTACGACCTGATTGTGTCAGCAAGGCTTTTGATTTGCACTGGGAGCTTTTTCTCGGGGGCGAT
CGCGGAGCGGGATTTAAAACTATGTTATCGGAATTAGGGGTTAACAATCTGTCATGGGAATTACAAGAGTTCTTGCGTCA
AGCGGAACAAACGCAATTCTCGGTGACTCAATTGCATCAACGCATGCGTGAAATCGATTTGTTTCGTCTGGAACTCGCTT
TATTCATGCAAGATTATGATGTATTGATTAGTCCAGTGTTTCCAACAGTAGCAAAACCGCATGGGATTGGGATTAAAGAA
ATCAGCGATTTTTCCTATGCGATGACACACAATTTAAGTGGATTTCCAACGATTAGCCTAAGATGCGGGACTTCAGCCGA
GGGGTTGCCCATTAATGTATTAATAGCCGCTAATCGCTGGAAAGATACTACCTCTCTGGCTGTTGCAGAACGCGTTGAGC
AATTAATGGGGGGATATAAACCCCCTTCTCTAATCCACTCTCTGCAATGA

Upstream 100 bases:

>100_bases
TTTGCAATAGTTTGAGACGGATACTGAATGTTGCTTTTTAGTCCTGTTCGGTGTGTCACTATCATTCTACTCCATTATTT
AAGGAAATTTTACTAAAGCT

Downstream 100 bases:

>100_bases
AGCAAGAATCAATAATTTTTGAAACAGCGAACTAGGGCAGAAACAAGGCTTGCAAATCATTAGTATATTGCCTGCCTAAT
GGTGTTACTTGCCAATGAGT

Product: amidase (enantiomer selective)

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 1 [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSISSKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDAEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
RGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAERVEQLMGGYKPPSLIHSLQ

Sequences:

>Translated_469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSISSKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDAEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
RGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAERVEQLMGGYKPPSLIHSLQ
>Mature_469_residues
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEIDKSISSKKNLNKLMGLPVAI
KDALYVKGLICSSACSGFYKGEKAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDAEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGD
RGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKE
ISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAERVEQLMGGYKPPSLIHSLQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=29.7570850202429, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=25, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI166795287, Length=507, Percent_Identity=25.4437869822485, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=29.2246520874751, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=31.6993464052288, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538252, Length=518, Percent_Identity=27.027027027027, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=479, Percent_Identity=26.3048016701461, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=313, Percent_Identity=32.9073482428115, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=32.9073482428115, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17538254, Length=453, Percent_Identity=28.476821192053, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=424, Percent_Identity=26.1792452830189, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI32565337, Length=304, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=34.297520661157, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=227, Percent_Identity=34.3612334801762, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=188, Percent_Identity=33.5106382978723, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=30.1810865191147, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21356731, Length=482, Percent_Identity=29.6680497925311, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=28.1059063136456, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=28.1059063136456, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=28.1059063136456, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=27.1084337349398, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI161078093, Length=486, Percent_Identity=29.0123456790123, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24648441, Length=423, Percent_Identity=27.6595744680851, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648439, Length=423, Percent_Identity=27.6595744680851, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648113, Length=476, Percent_Identity=26.0504201680672, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 50563; Mature: 50563

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKSISSKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGEKAVRDATLVSRLKKEGAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGGSAALIAAGGVPFALGSDGGG
EEEECCCHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCC
SLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
CCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDAEIQNVVKSAALALQDDVAIVREV
CCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
RPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQ
CHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKEISDFSYAMTHNLSGFPTISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHCCHHHHHHCCCCCCCEEEE
RCGTSAEGLPINVLIAANRWKDTTSLAVAERVEQLMGGYKPPSLIHSLQ
EECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINALTERIPPEECLKQAKEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKSISSKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGEKAVRDATLVSRLKKEGAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGGSAALIAAGGVPFALGSDGGG
EEEECCCHHHHCCCCCCCEEECCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCC
SLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNLISFGPMARSVSDLRLGLSVLAG
CCCCCHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVDAEIQNVVKSAALALQDDVAIVREV
CCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
RPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGVNNLSWELQEFLRQAEQTQFSVTQLHQ
CHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMREIDLFRLELALFMQDYDVLISPVFPTVAKPHGIGIKEISDFSYAMTHNLSGFPTISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHCCHHHHHHCCCCCCCEEEE
RCGTSAEGLPINVLIAANRWKDTTSLAVAERVEQLMGGYKPPSLIHSLQ
EECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]