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The map label for this gene is kdtA

Identifier: 52842550

GI number: 52842550

Start: 2642686

End: 2643951

Strand: Reverse

Name: kdtA

Synonym: lpg2340

Alternate gene names: 52842550

Gene position: 2643951-2642686 (Counterclockwise)

Preceding gene: 52842552

Following gene: 52842544

Centisome position: 77.81

GC content: 40.68

Gene sequence:

>1266_bases
CTGTTTATGCGTTTTGTGTATTCCTTTTTAATGTACTTACTGACTCCTTACTTGCTTTTTCGCCTGTGGCGGAAAGGTAG
AAAGTTGCCAGCTTACAGGCAAAGGATAGGAGAGCGTTTTTGTCTGGGAATACAAGAAAATGCACCAGTTGATGTATGGC
TTCATGCGGTTTCTCTGGGAGAAGTGATCGCGGCAACACCTTTAATTGATGCAATGCTCAACAAACATTGGTCAGTGTTA
GTTACCACAATGACCCCTACAGGTTCTGAACGAGTAAAATCTCGATTTGGTCATAAAGTAGCGCATCAATATTTACCCTA
TGATTTACCTTGGGTTCTCAAACGATTTTTTAAAAGAACGAAACCTCGTGTCGGGATCATTATGGAAACTGAATTGTGGC
CTAATTTAATTAAGCAGGCTCATGCTTCAGGAGTGGCATTATTTCTGGCGAATGGACGCTTATCTGATCGCTCCTTGCAA
GGATATCTAAAGCTTAAATTTTTATTTAAACCGGTTTTGAATCAATTTTCAGGAATTTTGACTCAAAGCAATGAGGATGC
TGAGAGATTCATTGCCTTGGGTGCAAATGCCGATTTAGTCCATGTATTAGGGAATATGAAGTTTGATTTGCAGATTGACT
CTGTTGATAGTAGTCAATACAGGGAATTAAAAAGTCATTGGGGAGAGGATAGACTTACCGTCATTGCAGCAAGCACTCAT
GACGACGAGGAGTCTCAGATTTTATCTCAGCTACCTCGTTTGCAGGAGGCTATTCCGGGGGTAGTGTTATTAATTGCTCC
GCGTCATCCAGAACGATTTCAAACGGTCTATCAGTTGAGTGTTCAAGCAGGATTTAATACTGGGTGTCGTAGCAATTTGG
ATACGATTTCCAGAGAAAATGAAGTTGTTATACTCGACAGCTTGGGTGAGCTTTTGGGATTTTATCAAATTAGTGATTTT
GCTTTTGTTGGCGGCAGCCTTGTCCCGGTTGGTGGTCATAATGTATTGGAGCCAATCGCGATGAATGTGCCTGTTTTAAG
CGGGAATCAAGTGCATAACTTTAAATCAATTTGCAGAGAGCTTAAAGAGGCTCAGGCAATTTTGCTAGTGAATCATGCCA
ATGAGTTGGTTGATGCAATTATCAAGTTATATCAGGATAAGGAATCTCAAAGCACTATGGTTGCAAACGCCAGTTCAGTC
CTTGAAAGTAATAAGGGCTCTGTAGTGAGGTATTTACAGAAAATTGAGTCAGTTTTGGGATATTAG

Upstream 100 bases:

>100_bases
TAATAATATAGATTGAGGACAACATAGTCTCTAATTGAATTGTAACATATCGCACAAGGAACTGTTTTAGGTATATACTT
TACCTTTTTGTGTTTGGTAC

Downstream 100 bases:

>100_bases
CTTGTTATAAAATTCTCTCAATATTGATTTCTTGTATCATACCAGAAAATTCATCAAAACATTTTTGAACTTGTTCAAGG
TGGAGTATCCATGGACAATG

Product: 3-deoxy-D-manno-oct-2-ulosonic acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MFMRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKHWSVL
VTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTKPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQ
GYLKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQYRELKSHWGEDRLTVIAASTH
DDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTISRENEVVILDSLGELLGFYQISDF
AFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDKESQSTMVANASSV
LESNKGSVVRYLQKIESVLGY

Sequences:

>Translated_421_residues
MFMRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKHWSVL
VTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTKPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQ
GYLKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQYRELKSHWGEDRLTVIAASTH
DDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTISRENEVVILDSLGELLGFYQISDF
AFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDKESQSTMVANASSV
LESNKGSVVRYLQKIESVLGY
>Mature_421_residues
MFMRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKHWSVL
VTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTKPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQ
GYLKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQYRELKSHWGEDRLTVIAASTH
DDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTISRENEVVILDSLGELLGFYQISDF
AFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDKESQSTMVANASSV
LESNKGSVVRYLQKIESVLGY

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=420, Percent_Identity=42.6190476190476, Blast_Score=342, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47485; Mature: 47485

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHH
EVIAATPLIDAMLNKHWSVLVTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRT
HHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
KPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQGYLKLKFLFKPVLNQFSGIL
CCCEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQYRELKSHWGEDRLTVIAASTH
CCCCCCHHHHEEECCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCC
DDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTISREN
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
EVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRE
CEEEEECHHHHHHHHHHCCHHEECCCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
LKEAQAILLVNHANELVDAIIKLYQDKESQSTMVANASSVLESNKGSVVRYLQKIESVLG
HHHCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
Y
C
>Mature Secondary Structure
MFMRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHH
EVIAATPLIDAMLNKHWSVLVTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRT
HHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
KPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQGYLKLKFLFKPVLNQFSGIL
CCCEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQYRELKSHWGEDRLTVIAASTH
CCCCCCHHHHEEECCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCC
DDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTISREN
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
EVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRE
CEEEEECHHHHHHHHHHCCHHEECCCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
LKEAQAILLVNHANELVDAIIKLYQDKESQSTMVANASSVLESNKGSVVRYLQKIESVLG
HHHCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]