Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 52842546

GI number: 52842546

Start: 2639259

End: 2640347

Strand: Direct

Name: prfA

Synonym: lpg2336

Alternate gene names: 52842546

Gene position: 2639259-2640347 (Clockwise)

Preceding gene: 52842545

Following gene: 52842547

Centisome position: 77.68

GC content: 42.06

Gene sequence:

>1089_bases
ATGAAGAAATCTCTTGAGTTAAAATTACAGCAAATGTTGGAGCGATACGAGGAAGTAGGCCGCTTACTGTCCGAAGCATC
CATTATTGCCGATCAAAACCAATTTAAGTCTTTATCAAAAGAATATGCCCAACTTGAACCAGTTTCCCAATGTTATGAGT
CGTATTTAGAGGCAAAAAATAATTTGGATTCTTTAAACGAATTATTGGAAAGTGATGATAAAGATTTAGCGACTATGGCT
GAAGAAGAGATTGACACTGTCAAGAAACAAATTGAGGAATTGGATGAACAATTACAATGGCATCTCATTCCTAAAGATCC
TGATGATGAACGCAATATTTATCTTGAAGTCAGAGCCGGCACAGGTGGAGACGAAGCAGCTATATTTGCCGGAGATCTCT
TTCGAATGTACAGCCGTTATGCAGAAAGCCAGGGTTGGCAAATTGAATTAATTAGCGCCAGTCATGGTGAACATGGAGGC
TACAAGGAAATAATTGCCAAAATCAGTGGGCAAGCGGTTTATTCACAACTCAAATTTGAATCAGGAGCTCACAGAGTACA
GAGAGTACCTGAAACTGAATCGCAAGGTCGTGTCCATACTTCCGCATGCACGGTTGCTATCATGCCGGAGGTGGATGAAA
TTAATGACATTCAGATAAATCCCGATGACTTACGCATCGATACTTACAGATCTTCCGGAGCCGGTGGACAGCATGTTAAC
AAAACAGATTCTGCCATTCGTATCACACACATTCCTACAGGAGTGGTTGTAGAGTGCCAGGATGAGCGTTCACAGCACAA
AAACCGTGCCAAAGCAATGTCCTTGTTAAAAACAAGATTATTAGATGCCGAAGTAAGCAAACAAAAACAAGAACAAGCTC
AAACACGTAAGTCACTGGTTGGTACAGGTGACCGCTCCGAGCGCATTAGAACTTATAACTTTCCTCAAGGCCGGCTCACT
GATCACCGTATCAATCTGACTATCTACCAGCTTAGTGACATCATGGAAGGGAATTTATCCCTGGTCATTGACCCGTTAAA
ACGCGAATACCATGCTGAACTGCTAGCCGATTTAGGTCGACATGATTGA

Upstream 100 bases:

>100_bases
AATCCCACCATAGGTTTAAGACAAATAGCATGGGATAACAGAGAAGACTTACTTGACCTCGCGCGATATCTCTTTGATAC
AACAGCCAATCAATCATTAT

Downstream 100 bases:

>100_bases
TATTCGTTCATTACTGGAACAAGCTCTGCAACAATTCAAAGTCAAAAACCAGGAAACCAAGTTAGAGGCAGAACTTTTAC
TCTGCCATGTGTTAAACAAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDTVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD

Sequences:

>Translated_362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDTVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD
>Mature_362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDTVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=285, Percent_Identity=49.1228070175439, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI34577120, Length=343, Percent_Identity=41.399416909621, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=49.746192893401, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=62.5698324022346, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=39.2215568862275, Blast_Score=231, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=33.9483394833948, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=306, Percent_Identity=42.156862745098, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=39.0909090909091, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_LEGPA (Q5X2V3)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124595.1
- ProteinModelPortal:   Q5X2V3
- SMR:   Q5X2V3
- STRING:   Q5X2V3
- GeneID:   3116974
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2284
- LegioList:   lpp2284
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   LPNE297246:LPP2284-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41103; Mature: 41103

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEC
TGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGGYKEIIAKISGQAVYSQLKFE
CCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
SGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCC
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGR
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHCC
HD
CC
>Mature Secondary Structure
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEC
TGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGGYKEIIAKISGQAVYSQLKFE
CCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
SGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCC
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGR
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHCC
HD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA