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The map label for this gene is grpE

Identifier: 52842243

GI number: 52842243

Start: 2268198

End: 2268800

Strand: Reverse

Name: grpE

Synonym: lpg2026

Alternate gene names: 52842243

Gene position: 2268800-2268198 (Counterclockwise)

Preceding gene: 52842245

Following gene: 52842242

Centisome position: 66.77

GC content: 40.63

Gene sequence:

>603_bases
TTGATGAGTAAACAAAATAAAAAAGATTGGAAAAAATTTAAAGACGAACACAAAGAAGAACACAAGGTAGAGAATGAGAT
TCTGGAAGAAGAAACTGATGAAGAATCACAACATCAAGAACCTGCTTTAGGACATCCCAGCTATACTGCCTTGGAAGAGC
AACTGACATTGGCGGAACAAAAAGCACATGAAAATTGGGAAAAGTCTGTACGCGCCCTTGCTGAATTGGAGAACGTTCGT
CGTCGTATGGAACGAGAAGTTGCCAATGCTCATAAATATGGCGTAGAAAAACTGATTTCAGCTTTATTGCCAGTAGTGGA
CAGTCTTGAGCAAGCATTACAATTAGCGGACAAGAACAGTGATCCATCTATGCATGAAGGACTTGAGTTAACTATGAAAC
TCTTTCTTGACGCATTACAGAAGTTTGATGTGGAACAAATTGATCCTCTTGGACAGACATTTGACCCTCAACAACATGAG
GCAATGTCAATGCAACCTGCCCCAGGAGCGCCACCGAATTCTGTAATTACTGTATTCCAAAAAGGTTACAAATTGAGCGA
TCGAGTAATTCGACCAGCTCGGGTGATAGTTTCCACCAAATAA

Upstream 100 bases:

>100_bases
AATGACCTGAATTTTAAACCAAGATTTAAAATCCTAACAGGGGCTTGAAAATTGCAAATACAATCCCCATTTGTAGGAAT
CGAGTTAGTCAGGAGCGATT

Downstream 100 bases:

>100_bases
TCTGGTGTTTCAGTTGAGGTATTTTTAAACAAATTTTTAGATGCAGTGTATTCAGCTTTTTTTAGGGTGATAGCTTGAAA
TCGTCATGATGCACCCCATA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MMSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVR
RRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHE
AMSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK

Sequences:

>Translated_200_residues
MMSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVR
RRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHE
AMSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK
>Mature_200_residues
MMSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQKAHENWEKSVRALAELENVR
RRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHE
AMSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=179, Percent_Identity=36.8715083798883, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=34.7826086956522, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1788967, Length=186, Percent_Identity=40.3225806451613, Blast_Score=121, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17552458, Length=143, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=193, Percent_Identity=32.1243523316062, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=34.8571428571429, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_LEGPH (Q5ZTY2)

Other databases:

- EMBL:   AE017354
- RefSeq:   YP_096042.1
- ProteinModelPortal:   Q5ZTY2
- SMR:   Q5ZTY2
- STRING:   Q5ZTY2
- GeneID:   3078731
- GenomeReviews:   AE017354_GR
- KEGG:   lpn:lpg2026
- NMPDR:   fig|272624.3.peg.862
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14144
- BioCyc:   LPNE272624:LPG2026-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22935; Mature: 22935

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEAMSMQPAPGAPPNSVITVFQ
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
KGYKLSDRVIRPARVIVSTK
HCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGHPSYTALEEQLTLAEQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALLPVVDSLEQALQLADKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEAMSMQPAPGAPPNSVITVFQ
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
KGYKLSDRVIRPARVIVSTK
HCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA