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The map label for this gene is yfcG [H]

Identifier: 52842187

GI number: 52842187

Start: 2210715

End: 2211338

Strand: Reverse

Name: yfcG [H]

Synonym: lpg1970

Alternate gene names: 52842187

Gene position: 2211338-2210715 (Counterclockwise)

Preceding gene: 52842188

Following gene: 52842186

Centisome position: 65.08

GC content: 37.66

Gene sequence:

>624_bases
ATGTATACGCTTTATTCATTTGGGACGCCCAATGGATTTAAACCCACTATTATGTTGGAAGAGTTAACGCAGCCTTACAC
CATAGAACTGATTGATATTACTCAGGGTGAACAATTTAATCCTGATTTTTTAAAAATTTCACCAAACAATAAGATACCCG
TTTTACATGATCAAACCAATGATTTTTACCTAATGGAAAGTGTTGCTATTTTGCAATATTTAGCTGAAAAACATAGACAG
TTTTTGCCTGAAACGACAAAGGAGAAATATACTATTATCCAATGGTGTTATTTCCAAGCAGCACACATAGGCCCTATGTT
CGGGCAGTTTGGTCATTTTCATCGTTATGCTCAAGAGCGAGTACCCTATGCCATGACGCGATATGCTGATGAATCAATGC
GTCTTCTAGGTGTTATGGAAAAACAACTGGTCAAAGCGCCATTCATTGGTGGATCAAATTATACTATTGCTGATATGGCT
ATCTGGCCCTGGATTTGGTGTTTTCAATTCATATATGGACAGACTATTGATGCAAAACAGTTTCCCAGTTTAATGTCATG
GTATCAACGAATTAGTGAAAGGCAGGCTGTTAGAGCAACTCTAACGGCATATGGTCGATTATGA

Upstream 100 bases:

>100_bases
GGTTTGTCCTTATTCCAATGCAATACGTGGTAATGTTGATGTGAAATTGAGTGTTAGTGTCAAGTAATAAATAACTTCCA
TCAATCAATAAGGATATCTT

Downstream 100 bases:

>100_bases
TCACGAATCTCCATTAGAAGTAAGCGAGCAAAGTTGAACTTATCTGATAATTAATGGCTCCCAATTGAGGTAATGGTGTC
AAGTGGGGTGTTTTACCTGG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFLKISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQ
FLPETTKEKYTIIQWCYFQAAHIGPMFGQFGHFHRYAQERVPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMA
IWPWIWCFQFIYGQTIDAKQFPSLMSWYQRISERQAVRATLTAYGRL

Sequences:

>Translated_207_residues
MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFLKISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQ
FLPETTKEKYTIIQWCYFQAAHIGPMFGQFGHFHRYAQERVPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMA
IWPWIWCFQFIYGQTIDAKQFPSLMSWYQRISERQAVRATLTAYGRL
>Mature_207_residues
MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFLKISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQ
FLPETTKEKYTIIQWCYFQAAHIGPMFGQFGHFHRYAQERVPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMA
IWPWIWCFQFIYGQTIDAKQFPSLMSWYQRISERQAVRATLTAYGRL

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=42.7184466019417, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI87082195, Length=207, Percent_Identity=40.0966183574879, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI87081789, Length=173, Percent_Identity=30.0578034682081, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324100, Length=241, Percent_Identity=29.045643153527, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571670, Length=185, Percent_Identity=29.1891891891892, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17864598, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 24346; Mature: 24346

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFLKISPNNKIPVLHDQTN
CEEEEECCCCCCCCCEEEHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEECCCC
DFYLMESVAILQYLAEKHRQFLPETTKEKYTIIQWCYFQAAHIGPMFGQFGHFHRYAQER
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMAIWPWIWCFQFIYGQTIDAKQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHH
FPSLMSWYQRISERQAVRATLTAYGRL
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFLKISPNNKIPVLHDQTN
CEEEEECCCCCCCCCEEEHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEECCCC
DFYLMESVAILQYLAEKHRQFLPETTKEKYTIIQWCYFQAAHIGPMFGQFGHFHRYAQER
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMAIWPWIWCFQFIYGQTIDAKQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHH
FPSLMSWYQRISERQAVRATLTAYGRL
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]