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The map label for this gene is xasA [C]

Identifier: 52842135

GI number: 52842135

Start: 2139383

End: 2140804

Strand: Direct

Name: xasA [C]

Synonym: lpg1917

Alternate gene names: 52842135

Gene position: 2139383-2140804 (Clockwise)

Preceding gene: 52842134

Following gene: 52842142

Centisome position: 62.96

GC content: 40.72

Gene sequence:

>1422_bases
ATGAAATCATGGTCCTCTGAAAAAATATCCGTTCTTGCTTTAGTGCTGCTCATTACCGGAGCAATTGACAGCATTAGAAA
TTTGCCAGGCACAGCACTTTTTGGATCTACTTTGATTTTTTTCTTTATTTTTTCAGCAATTATCTTTTTGATCCCGGTTG
CTTTAGTCGCTGCTGAACTTTCCTCAACCTGGTCAGACGAGGAAGGTGGCATTTACAGTTGGGTCAAGCATGCCTTTGGT
GAAAACATCGCCTTTTTCGCCATTTGGCTGCAATGGATAAATACTCTGGTATGGTATCCGACTATTTTGTCTTTTATAGC
AGGCACAATCTCTTATTTAATTAATCCAGAACTTGCACAAAACAAATACTATCTTATCAGCGTTATTCTGATTATTTTCT
GGTCATTAACTTTGATAGGCTTATCAGGACTGCGTGCTTCTGCCACCTTTGCTGGCTTCTGTGCAATAGTTGGAATGATA
GTGCCTATGGGATTTATCATCCTTCTGGCTTTTATTTGGTTAATTCATGGCCACCCTATTGCCATTGATCTGAGCCTCTC
CAATTTGCTACCGCAATGGAAAAACACACAATCCTGGGTTTCTCTAACAGCGATTATGACTTCTTTTCTCGGGATGGAGC
TTGCTGCAGTTCACGTGCGTAATGTCAATAACCCGCAGAAGAATTTTCCCAAAGCGATGTTTTTTTCGGTCATTCTCATC
CTGTTCACCATGATTTTAGGCTCACTGGCCATTGCTTTCATTCTGCCTAAAGACAAAATCAGTTTGGTAGATGGGGTTAT
GAGGGCATTTAGTAATTTCTTGCAAGTTTATGATTTGAATTGGCTAATGCCTGTATTAATACTCTTGTTATTGCTGGGTA
CCTTAGGCAGCATGATCAACTGGATTATTTCTCCAGCCAAGGGCCTGCTGATGGCAGCCAAACATGATTTTCTGCCTGCT
TCGCTATGTCATGTGAATAAACATGGCATGCCAGCAAAAATTTTGATACTGCAAGCCATTCTGGTGACAGTATTATGTAG
CGGCTTTCTCTTATTCCCTACAGTGAATGCTATTTATTGGTTGTTTACTGCTCTTAGTACAGAGCTGTATATGATGATGT
ATGTGATGATGTTTATTGCAGCCTGGCATTTGAAGAGTAAATTCTCAGACATGGAAAGACCTTTTGCTATTCCAGGAGGC
AAACCCGGTTATTACCTGACATGCCTGTTAGGATTAATAGGTTGTACTGTCACTTTATGGGTAGGTTTTATTCCTCCAAC
GGAAAGCATTAATATCGGAGGAACAGCCCATTACCGTATGGTCTTTTCATTAGGCATCGTCTTAATGCTGCTACCTGCTG
CCTTGATCTATTTGCGTAAACGCCACATTACCAAATCCAAAGCGCTTAATGCACCTGTTTAA

Upstream 100 bases:

>100_bases
GCAGAGATGGCTATAAAAACAAATAATTTTAATGAAAAAAGTTCACCTCCAAGCTTGATTTATTTTATAATGTCTATCTT
TTTATCAAATAGAAAAATCA

Downstream 100 bases:

>100_bases
ATCATCGATAGGATGGTTGTAACCCGTGGCTTGTCCACAGGGTCAGTGTTTTAGCGGAGAGCTCCTGGATCCCGTGGACA
AGCCACGGGACGTCGAAGAT

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIFSAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFG
ENIAFFAIWLQWINTLVWYPTILSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVHVRNVNNPQKNFPKAMFFSVILI
LFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQVYDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPA
SLCHVNKHGMPAKILILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDMERPFAIPGG
KPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGIVLMLLPAALIYLRKRHITKSKALNAPV

Sequences:

>Translated_473_residues
MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIFSAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFG
ENIAFFAIWLQWINTLVWYPTILSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVHVRNVNNPQKNFPKAMFFSVILI
LFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQVYDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPA
SLCHVNKHGMPAKILILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDMERPFAIPGG
KPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGIVLMLLPAALIYLRKRHITKSKALNAPV
>Mature_473_residues
MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIFSAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFG
ENIAFFAIWLQWINTLVWYPTILSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVHVRNVNNPQKNFPKAMFFSVILI
LFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQVYDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPA
SLCHVNKHGMPAKILILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDMERPFAIPGG
KPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGIVLMLLPAALIYLRKRHITKSKALNAPV

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=442, Percent_Identity=28.9592760180996, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI87081798, Length=368, Percent_Identity=24.4565217391304, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082380, Length=432, Percent_Identity=24.7685185185185, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52472; Mature: 52472

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIFSAIIFLIPVALVAAEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTILSFIAGTISYLINPELAQ
HHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
NKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMIVPMGFIILLAFIWLIHGHPI
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVHVRNVNNPQKNFPKAMFFSVILI
EEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHH
LFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQVYDLNWLMPVLILLLLLGTLGSMIN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIISPAKGLLMAAKHDFLPASLCHVNKHGMPAKILILQAILVTVLCSGFLLFPTVNAIYW
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LFTALSTELYMMMYVMMFIAAWHLKSKFSDMERPFAIPGGKPGYYLTCLLGLIGCTVTLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VGFIPPTESINIGGTAHYRMVFSLGIVLMLLPAALIYLRKRHITKSKALNAPV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIFSAIIFLIPVALVAAEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTILSFIAGTISYLINPELAQ
HHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
NKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMIVPMGFIILLAFIWLIHGHPI
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVHVRNVNNPQKNFPKAMFFSVILI
EEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHH
LFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQVYDLNWLMPVLILLLLLGTLGSMIN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIISPAKGLLMAAKHDFLPASLCHVNKHGMPAKILILQAILVTVLCSGFLLFPTVNAIYW
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LFTALSTELYMMMYVMMFIAAWHLKSKFSDMERPFAIPGGKPGYYLTCLLGLIGCTVTLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VGFIPPTESINIGGTAHYRMVFSLGIVLMLLPAALIYLRKRHITKSKALNAPV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]