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The map label for this gene is paaF [H]

Identifier: 52842055

GI number: 52842055

Start: 2046969

End: 2047817

Strand: Direct

Name: paaF [H]

Synonym: lpg1828

Alternate gene names: 52842055

Gene position: 2046969-2047817 (Clockwise)

Preceding gene: 52842054

Following gene: 52842056

Centisome position: 60.24

GC content: 39.1

Gene sequence:

>849_bases
TTGAGGATACTCGTTTCGGTGTATTTCGCATGTAAAAGGAAAGGAACAATAGGCATGAGTGATTTATTATATGAAATTCA
AGATAAAGTAGGTTTACTTACTATGAATCGAATTAGCAAGCATAATGCGTTCGATAATCAACTGCTGACTGAAATGAGAA
TACGACTTGACTCCGCAATAAATGATACTAACGTTCGCGTCATAGTACTTAAAGCGAATGGAAAGCATTTTTCAGCTGGT
GCTGATTTGACCTGGATGCAAAGCATGGCTAACTTTACTGAAGAAGAAAACCTGGAAGACAGTCTGGTATTAGGTAATTT
AATGTATAGCATCAGTCAAAGCCCAAAACCTACTATCGCCATGGTTCAAGGTGCCGCTTTTGGTGGTGGAGCTGGATTGG
CAGCAGCATGTGATATAGCCATTGCATCTACTTCAGCACGTTTTTGTTTTTCTGAAGTGAAATTAGGTCTTATTCCAGCA
GTAATTAGTCCCTATGTAGTTAGAGCAATAGGTGAACGAGCGGCAAAAATGTTATTCATGAGTGCTGAAGTTTTTGATGC
CACACGTGCATATTCTCTTAATTTAGTTCAGCACTGCGTCCCTGATGATACGTTGTTGGAGTTTACTCTCAAATACGCCT
CGCAAATCAGTAATAATGCTCCAGAAGCGGTTAAAAACTCAAAACAACTGGCACAATACGTCGCTAATAAAAAAATAGAT
GAAGAACTGGTTCGCTACACTGCTTCTCTGATTGCTCATAAAAGAGTATCTGATGAGGGGCAAGAAGGGCTTAAAGCATT
TCTCAATAAAGAAATACCCAACTGGAATAAAGGTTCTGGACATGTTTAA

Upstream 100 bases:

>100_bases
TACTATGCCAGTGCCAGGCTTTGGGATGATGGGGTCATCGCGCCTCAGGATACACGCAAAATACTCGGACTTGGATTATC
TGCAGCCTTAAACGCACCGA

Downstream 100 bases:

>100_bases
TAAAATATTAATTGCTAACCGAGGCGAAATAGCCTGTCGAATCATTAAAACAGCACATTCTATGGGTATTCAGGCTATTG
CCGTTTATTCAGCAGCAGAT

Product: enoyl CoA hydratase/isomerase (crotonase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRILVSVYFACKRKGTIGMSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAINDTNVRVIVLKANGKHFSAG
ADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIAMVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPA
VISPYVVRAIGERAAKMLFMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVANKKID
EELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV

Sequences:

>Translated_282_residues
MRILVSVYFACKRKGTIGMSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAINDTNVRVIVLKANGKHFSAG
ADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIAMVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPA
VISPYVVRAIGERAAKMLFMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVANKKID
EELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV
>Mature_282_residues
MRILVSVYFACKRKGTIGMSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAINDTNVRVIVLKANGKHFSAG
ADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIAMVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPA
VISPYVVRAIGERAAKMLFMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVANKKID
EELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=30.4, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=27.5193798449612, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68989263, Length=196, Percent_Identity=30.6122448979592, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=30.3418803418803, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=271, Percent_Identity=27.6752767527675, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI45643119, Length=271, Percent_Identity=27.6752767527675, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=271, Percent_Identity=27.6752767527675, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=29.5275590551181, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=173, Percent_Identity=26.0115606936416, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=32.824427480916, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=28.5171102661597, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=28.90625, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=27.0992366412214, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=26.5625, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=33.8308457711443, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.0395256916996, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=29.5275590551181, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=25.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=26.4, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=267, Percent_Identity=25.0936329588015, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=27.1111111111111, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=222, Percent_Identity=25.2252252252252, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=31.640625, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=30.2040816326531, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=30.2040816326531, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920382, Length=211, Percent_Identity=32.7014218009479, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=29.3478260869565, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30931; Mature: 30931

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVSVYFACKRKGTIGMSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAI
CEEHEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
NDTNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIA
CCCCEEEEEEEECCCEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEE
MVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKMLFM
EEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVANKKID
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHH
EELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRILVSVYFACKRKGTIGMSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAI
CEEHEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
NDTNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIA
CCCCEEEEEEEECCCEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEE
MVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKMLFM
EEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVANKKID
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHH
EELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]