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The map label for this gene is iolD

Identifier: 52841878

GI number: 52841878

Start: 1822391

End: 1824262

Strand: Reverse

Name: iolD

Synonym: lpg1650

Alternate gene names: 52841878

Gene position: 1824262-1822391 (Counterclockwise)

Preceding gene: 52841879

Following gene: 52841877

Centisome position: 53.69

GC content: 40.44

Gene sequence:

>1872_bases
ATGAAGATTAAATTAACTATGGCTCAGGCCCTATTAAGATTTTTAGCTAATCAGTATGTCGTTCTGGATAATAATGAATA
CCGTTTCATCAATGGTGTATTTGGAATATTTGGCCATGGTAATGTAACCGGATTAGGTGAGGCATTGGAATACGATGCTC
AGGGTTTAATTTATTATCAGGGACATAATGAACAAGGAATGGCTCACGCAGCAACCGCCTATGCCAAACAAAAAAATCGG
CTAGGGATAATGGCTTGTACTTCATCAATTGGTCCTGGCGCTACTAATATGATTACTGCAGCGGCGACAGCGACAACCAA
TAGAATTCCATTACTGTTGTTGCCAGGTGATGTATTCAGTTGCAGGCAACCAGATCCGGTTCTCCAACAATTGGAGGTGC
CATATGACTACACCATATCTGTTAATGATTGCTTTAAACCAGTAAGTAAGTTTTGGGACAGGATAACCAGACCTGAACAG
TTAATGACAGCGTGTATGCATGCTATGAGGGTATTAACCGATCCAGTTGAAACAGGTACTGTTACTTTATGTTTGCCTCA
AGACGTTCAGTCTGAAGCTTATGAATATGAGGAATCTTTTTTTAAAAAGCGCTTATGGCGTATTGAGAGAGAGAGGATAA
GTGATGCAATGTTGACAGAGGCCATAAGGCATATCAGAACGGCTAAGCAACCATTAATCATTGCAGGTGGTGGTGTACAT
TATTCACTGGCTACGGAGACTTTATCAGAGTTTGCATTAAAACATCGCATTCCTGTTGCTGAAACCCAAGCAGGAAAAAG
TAGCCTGACAGCGAAACACCCCATGAACGTTGGTGGAATAGGTGTTACTGGATCTGAAATAGCGAATTCTCTGGCAGCTC
AGGCTGATGTTATTTTGGTTATCGGGTCTCGATTACAGGATTTTACGACAGCTTCCAAATGGGCTTTTAAAAACCAGGAC
TGCCAAATCATTCATCTTAATGTATCACGTCTTGATGCCATGAAAATGAATGGAGTTATGTTAAAAGGAGATGCTAAAGC
TGGTTTGGAGCAATTGGGGAAAGAGCTGGCAGATTATCAAACCTCTCTCTCTTATCAGCAGTTAATACGTCATTATCAAA
ATGAATGGGCTCAAGAGTTAGATAAATTGACTCAGTCTTATTCTACAGAGCAGGCTGGGTTACACCAAACTACTGTTTTG
GGACTGCTCAATCAGTATGTTACTGAAGAGGATGTGATAATTAGTGCAGCGGGGAGTTTGCCAGGTGATTTGCATCGAAT
TTGGCAATCCAAAAAGGCAAAGGATTATCATTTGGAGTACGCTTATTCCTGTATGGGATATGAGGTTGCAGCGGGTCTAG
GAGTACGTATTGCAAAAGAAAATCAGGAAGGAGAGGTCTATGTTTTAGTTGGAGATGGCAGTTTTGTCATGATGCATTCA
GAGCTTTTAACTGCCATCCAGGAACGCAAGAAAATTACAATTCTCATTTTTGATAACCATGGATTTCAGTGCATTAGAAA
TTTACAGGAAGGGAATGGTAGTCTGGGTTTTGGTAATGAATTTCGTTATAGGGAGCCAACTACTAATGCTTTAAATGGCG
ATTATTTACCCATAGATTTTTGTAAGTACGCAGAGGGATTGGGGGCAGCTACTTATTTTGTTAAATCCTATGAGCAATTC
CGGACTACTTTAGACTCAGCAAAAAAACAGGAAAATAGTACGGTTATTGTTCTTCCTGTATTACCAAAGACCATGAGTCA
AGGATATAAGACTTGGTGGCGGGTTGGAGTTGCTGAGGTATCTCAATCGGAATCAGTGAGTAAAGCTTACCAAATGATGC
GAGAGCAAATTGAAACAGTTAGACAATATTGA

Upstream 100 bases:

>100_bases
AAGACCATGAATGGACAAGGACAAGCAGGGCAAATTTAAGAACATGGCAACATAAACAGTAGTAATCCTTATCTGCAGTA
TGATTTTGAGAGTCAAATAA

Downstream 100 bases:

>100_bases
AGATGGAAGTGAAGATGAGTATTCAACTTGGTATCGCACCAATTAATTGGTGCAATGATGATGATCCGAATTTAGGTAAA
GACATTAGTTTTGAGCAATG

Product: myo-inositol catabolism protein IolD

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLTEAIRHIRTAKQPLIIAGGGVH
YSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKAGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTTVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFVMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTTLDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY

Sequences:

>Translated_623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLTEAIRHIRTAKQPLIIAGGGVH
YSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKAGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTTVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFVMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTTLDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY
>Mature_623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLTEAIRHIRTAKQPLIIAGGGVH
YSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKAGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTTVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFVMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTTLDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=23.2682060390764, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790104, Length=510, Percent_Identity=24.7058823529412, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=23.8010657193606, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788716, Length=509, Percent_Identity=26.1296660117878, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786717, Length=523, Percent_Identity=21.7973231357553, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787096, Length=445, Percent_Identity=19.7752808988764, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=23.091976516634, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531301, Length=511, Percent_Identity=23.091976516634, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=24.1197183098592, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922626, Length=602, Percent_Identity=22.093023255814, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69565; Mature: 69565

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQ
CEEEEHHHHHHHHHHCCCEEEEECCCEEEEECEEEECCCCCCCCCCHHHHCCCCEEEEEE
GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC
CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLTEAIRHIRTAKQPLIIAGGGVH
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
YSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMNVGGIGVTGSEIANSLAAQADVILV
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEE
IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKAGLEQLGKELADYQ
ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
TSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTTVLGLLNQYVTEEDVIISAAGSL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCC
PGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFVMMHS
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEECCCCEEEEHH
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALNGDYLPIDF
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CKYAEGLGAATYFVKSYEQFRTTLDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SQSESVSKAYQMMREQIETVRQY
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQ
CEEEEHHHHHHHHHHCCCEEEEECCCEEEEECEEEECCCCCCCCCCHHHHCCCCEEEEEE
GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC
CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLTEAIRHIRTAKQPLIIAGGGVH
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
YSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMNVGGIGVTGSEIANSLAAQADVILV
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEE
IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKAGLEQLGKELADYQ
ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
TSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTTVLGLLNQYVTEEDVIISAAGSL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCC
PGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFVMMHS
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEECCCCEEEEHH
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALNGDYLPIDF
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CKYAEGLGAATYFVKSYEQFRTTLDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SQSESVSKAYQMMREQIETVRQY
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA