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The map label for this gene is thi3

Identifier: 52841795

GI number: 52841795

Start: 1731264

End: 1732214

Strand: Direct

Name: thi3

Synonym: lpg1565

Alternate gene names: NA

Gene position: 1731264-1732214 (Clockwise)

Preceding gene: 52841792

Following gene: 52841796

Centisome position: 50.95

GC content: 39.75

Gene sequence:

>951_bases
ATGGCGATGTCATCACTAAAATCCAGGGTTACCCTACTGCTTAATTGGTACACCAACCCTTATCATACCCCCATACTTGT
GGCACAACAATTGGGATTTTATTCGGAAGAAGATATCAAGCTCGCCATTCTGGAGCCTGCAGATCCCAGTGATGTGACCG
AAATTGTTGGCCTGGGAACAGTAGATTTCGGTGTTAAAGCAATGATTCATACTGTTGCAGCCAAAGCAAAAGGTTATCCA
GTTACTTCCATAGGAACGCTTCTTGATGAGCCCCCTACCGGACTTATTGCTTTAAAATCAAGTGGCATCAATTCTTTCCA
GGATATTGTTGGGAAACGAGTAGGTTATATCGGCGAATTCGGCAAAAAAATTATTGATGATTTGGCCAGCCTCGCAGGTA
TTGATCCGACGAGCTACAAAACAGTACGTATTGGCATGAATGTCACAGATGCCATTTATCGGGATGTTATCGACACGGGT
ATTGGTTTTATTAATTTTCAAAAAGTTGAACTGGAGCATCTTTGCGGAGAAACTGTATTTTTACGAATCGATCAATTAGC
TGGTTTAGGCTGTTGTTGTTTCTGCTCAATACAATTTATTGTTCCTGAAATAACATTACAGCAACCCGAATTAGTCAAAG
GATTTCTCAGAGCAACACAGCGCGGTGCAGCCTACACGACAGAAAAACCTGAAGAAGCCTATGAGTTATTATGTCAGGCT
AAACCACAGTTGCGGACTCCTTTATATCAAAAAATTTTTACAAGAACATTACCATTTTTTTCACGTACATTAATTAATGT
TGACAGAGACTGGGATAAGGTTGGACGCTATACCAAGCACCTAAAAATTATTGATGAACATTTTGATATCTCGCAATGCT
ATACGAATCGTTTTTTGCCAGATACACCTTATTCTGATTTGAAACCAATCGCCTGTTGTCTTGAAAATTAA

Upstream 100 bases:

>100_bases
CATATCAGCGTAAGGACGCCTTCAAAAAAACCATGGCTTTTTTAAAGCATGGTTCCCTTTTGCCATCTCCATAATATTTA
AAATAATAACATAAGGAGTT

Downstream 100 bases:

>100_bases
TTATGCGAGCAGGCATTGTAGGCTCAGGGATCATGGGGCGTCTGCTGGCTTTTGTATTACATAATGCGGGCTGGCAGGTA
TCCCTTTTTGATCGCTCTAA

Product: thiamine biosynthesis protein NMT-1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAMSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYP
VTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTG
IGFINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQA
KPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN

Sequences:

>Translated_316_residues
MAMSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYP
VTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTG
IGFINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQA
KPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN
>Mature_315_residues
AMSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPV
TSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTGI
GFINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQAK
PQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN

Specific function: Probably involved in thiamine biosynthesis [H]

COG id: COG0715

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NMT1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322616, Length=300, Percent_Identity=47.3333333333333, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321050, Length=300, Percent_Identity=47, Blast_Score=271, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6319957, Length=300, Percent_Identity=47, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6323997, Length=300, Percent_Identity=46.6666666666667, Blast_Score=270, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35276; Mature: 35145

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGT
CCHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCC
VDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
GKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTGIGFINFQKVELEHLCGETVF
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEE
LRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQA
EEEHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
KPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDEHFDISQCYTNRFLP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
DTPYSDLKPIACCLEN
CCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AMSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEPADPSDVTEIVGLGT
CHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCC
VDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGINSFQDIVGKRVGYIGEF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
GKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTGIGFINFQKVELEHLCGETVF
HHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEE
LRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQRGAAYTTEKPEEAYELLCQA
EEEHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
KPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYTKHLKIIDEHFDISQCYTNRFLP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
DTPYSDLKPIACCLEN
CCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]