Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 52841777

GI number: 52841777

Start: 1711279

End: 1712439

Strand: Reverse

Name: rlmN [H]

Synonym: lpg1547

Alternate gene names: 52841777

Gene position: 1712439-1711279 (Counterclockwise)

Preceding gene: 52841778

Following gene: 52841776

Centisome position: 50.4

GC content: 36.86

Gene sequence:

>1161_bases
TTGGGAGCGGTAATGGACCAACAAAAAGTGAATCTATTAAATTATAATTACTCACAGCTACGAGAATTATTGATAGCGTG
GGATGAAAAACCTTTCAGAGCTCAACAGCTATTTCAATGGATTCATCAAGTTGGCATACGTGACTTTGCTCAGATGACTA
ATTTAGGGAAAGTTTTAAGAAATAAACTTTCCCAATTAGCTTGTATAGATTTACCTGAAATCGTTGCATGTCAAAAATCA
GCTGATGGCACACATAAGTGGCTATTAAAGCTGGAATGTGGGAATTGTATTGAAACAGTGTTCATTCCGGAAGCAAATCG
AGGGACACTTTGTGTTTCATCACAAGTAGGTTGTGCTTTAAATTGTTCTTTTTGCTCCACAGCAAAGCAAGGATTTAACA
GAAATTTATCAACAGCTGAAATAATTGGTCAAGTATGGCTGGCAGCACGCGAACTATCAGATAATAATGGCACTCACGAT
AAAAAAATAACTAACGTCGTGATGATGGGAATGGGTGAACCGTTACTTAATTTCGATAATGTTGTTTCTGCTATGAACAT
CATGATGGATGATTTAGCCTATGGACTATCCAAAAGACGGGTTACATTAAGCACTTCGGGTGTTTTACCGGAAATGGAAC
GACTCAGAGAAGTCAGTCCTGTCGCGTTGGCAGTATCCTTACATGCACCAACAGATGAACTACGTAACGAGCTGGTACCA
ATTAATAAAAAATATCCTCTCTCTCAATTAATATCCCTATGTAAACGATATTTTAAAGACGAACCAAGAAGAAAAGTTAC
TTTTGAATACGTCATGCTAAAAGGAGTTAATGATCAACCTGAACATGCTAGTCAATTAATTAAACTTTTGCATAATGTGC
CTGCAAAAGTTAATTTAATTCCTTTTAACCCATTTCCTTTAACTCAATATCAGAGATCATCCCGGGAAACAATTGATGCC
TTTCGAGATAAATTAATGAAACATGGTATTAATACTATTACTCGCAAAACTCGTGGAGATGATATTGATGCAGCATGCGG
CCAACTTGCTGGCGAGGTAAAAGATAAAACGAGCAGATCACAACGATGGCAAAAACTTCATTTCATGAGCAAAACAGACA
AATCAACTGAATTGACCATTTCTTCGGAAGAAATTGCATAA

Upstream 100 bases:

>100_bases
ATGCAAATGCTGTGCATGGTTCTGACAGCCTTGAAAATGCTGCTCGTGAAATAGCATTTTTCTTTGAACAGCATGAGTTG
TGCAATAGATAAAGGCAGGC

Downstream 100 bases:

>100_bases
CACAGTCTTGTATAATAACCGAGAGCCAATTAAGAACTTTCTCCAGTCTTCCTGGTTGGAGATTATGTATAATAAAGGTG
TTTTCACCTGCAGACTCACA

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MGAVMDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKS
ADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHD
KKITNVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVP
INKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDA
FRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA

Sequences:

>Translated_386_residues
MGAVMDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKS
ADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHD
KKITNVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVP
INKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDA
FRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA
>Mature_385_residues
GAVMDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSA
DGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDK
KITNVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPI
NKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAF
RDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=356, Percent_Identity=56.7415730337079, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43619; Mature: 43488

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVMDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLR
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCAL
HHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEE
NCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKITNVVMMGMGEPLLNFDN
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHH
VVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCC
INKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLI
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
PFNPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRS
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QRWQKLHFMSKTDKSTELTISSEEIA
HHHHHHHHHHCCCCCCEEEECHHCCC
>Mature Secondary Structure 
GAVMDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLR
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCAL
HHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEE
NCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKITNVVMMGMGEPLLNFDN
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHH
VVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCC
INKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLI
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
PFNPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRS
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QRWQKLHFMSKTDKSTELTISSEEIA
HHHHHHHHHHCCCCCCEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA