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The map label for this gene is pilD

Identifier: 52841754

GI number: 52841754

Start: 1687745

End: 1688614

Strand: Direct

Name: pilD

Synonym: lpg1524

Alternate gene names: 52841754

Gene position: 1687745-1688614 (Clockwise)

Preceding gene: 52841753

Following gene: 52841758

Centisome position: 49.67

GC content: 35.17

Gene sequence:

>870_bases
GTGTCAATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGG
AAGTTTGCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCC
ATCTTGAACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTT
AAAGCGTGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAAT
CCGCTACCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTAT
TATTTGCTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTC
ACTTTAGGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTG
CGCTGGCGCTTATTTAGCCTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAG
ATTTCAAATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACG
GGGGCTATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTG
CATTTCTGGATTAATAGCTATGTTTTGGGGAGACTCAATCATCAATTGGTATATAGGTTATTGGATGTAA

Upstream 100 bases:

>100_bases
GGTTGGGGGATTGGTGGTGGCTATGTATTTACCTATTTTCAAACTGGGTTCTGCTGTTTAAACAGATCCTATTTAATTTA
ATCATACAATATAGGCAAAC

Downstream 100 bases:

>100_bases
AATGTTGATTAGCTTAGCGCAACCCAACAATAAATAGAGACCACACGCCTAAAATCACATGAGAAACAGATACTCCCAAA
AGAGATTTCTGTCTGTGAAA

Product: type 4 (IV) prepilin-like protein leader peptide processing enzyme PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSMINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMV
KAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSL
TLGLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSIT
GAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Sequences:

>Translated_289_residues
MSMINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMV
KAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSL
TLGLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSIT
GAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
>Mature_288_residues
SMINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVK
AWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLT
LGLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITG
AIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=272, Percent_Identity=37.1323529411765, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789732, Length=223, Percent_Identity=33.6322869955157, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32988; Mature: 32857

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLS
HEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHH
LYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCHHEEEH
DVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSIT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
HHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKE
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLS
HEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHH
LYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCHHEEEH
DVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSIT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM
HHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9529113 [H]