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The map label for this gene is trpA

Identifier: 52841536

GI number: 52841536

Start: 1433825

End: 1434643

Strand: Direct

Name: trpA

Synonym: lpg1305

Alternate gene names: 52841536

Gene position: 1433825-1434643 (Clockwise)

Preceding gene: 52841535

Following gene: 52841537

Centisome position: 42.2

GC content: 40.78

Gene sequence:

>819_bases
ATGAACCGTATTGATAAGACTCTGGAAAAATTAAAAGCCAATAGAAAAAAAATGTTAAGTCCTTATATTACTGCAGGCGA
TCCATATCCTGAGTTAACAGTAAGTTTAATGCATCAATTGGTTAAATCAGGGGCAGATGTATTGGAGCTGGGAATACCCT
TTTCTGACCCTATGGCAGAAGGTCCTGTTATCCAAAGAGCAATGGAACGAGCTTTGGCGCATTCAATTCATTGTGATGAC
GTATTGAATATGGTTAGGCAATTTCGCAAAACGGATACCGAAACGCCTGTGATTCTTATGGGATATTTAAATCCTATAGA
ACAATATGGCTATGATCTTTTTGCCCAGCAGGCAGTGGAGGCTGGGGTTGATGGAACAATATTAGTTGATTTGCCTCCTG
AGGAAGCTGATGGAGTATCGCGAGTTTGGCAAAAACATGGTTTGTACAGTATTTATTTATGTTCGCCAACCACCTCAGCT
GAAAGAATGAACTATATAAATCAGCATGCCAATGGTTATCTGTATTATGTTTCATTGAAGGGAGTGACAGGTTCTGACGC
GCTTAAGTTGCCAGAATTAAAAGCTCAATATCTACAACGAAAGGCACAATCAAAATTACCGCTTATGGTTGGGTTTGGAA
TAAAAACTCCGGAAATGGCTGCACAAGTTGCTGAATTTGCTGATGGGGTCATTGTTGGGGCGGCACTGATAAATGAAATC
ATTGAAGCCTATGAGGCCAAGAAAGATCCGCTGCAAGCAAGCGGGGCTCTATTGAGTTCCATGAGGCAAGCTATCGATAA
TATTGGAAGTATGGTATGA

Upstream 100 bases:

>100_bases
ATCATAGTCAATCTATCTGGTCGTGGTGATAAAGATATGCATACAGTAGCTGCTATTGACGGTATAACAATTTAATTTTG
ATAAATTATTCAGGTACAAC

Downstream 100 bases:

>100_bases
CAGAACAAACTGAAAAAAGAGCTCACTTTATTAGACAACTCATTATAGATGATTTGGCAAATGGAAAACATCAGGGAATC
GTTACTCGTTTCCCTCCTGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNYINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV

Sequences:

>Translated_272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNYINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
>Mature_272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNYINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=250, Percent_Identity=32.8, Blast_Score=138, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=249, Percent_Identity=38.9558232931727, Blast_Score=170, Evalue=3e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29937; Mature: 29937

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA ; PS00201 FLAVODOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHH
AGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSAERMNYINQHANGYLYYVSLK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHH
AGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSAERMNYINQHANGYLYYVSLK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA