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The map label for this gene is eco47IIM [H]

Identifier: 52841468

GI number: 52841468

Start: 1364928

End: 1366178

Strand: Direct

Name: eco47IIM [H]

Synonym: lpg1236

Alternate gene names: 52841468

Gene position: 1364928-1366178 (Clockwise)

Preceding gene: 52841464

Following gene: 52841473

Centisome position: 40.17

GC content: 41.09

Gene sequence:

>1251_bases
ATGAGCTACGATCTTATTGAAGATGCTCCTTTTGTTTCAATCAGTGAAGCAGCAACTGCACTGGATGCTTCTATCGACAC
CATAAGGCGTTGGGAAAAGAAAGGATTAATAAAGGCACACAGGAGTGATACAGGGCATCGGCTGTTTCAAATAACTGAAC
TCCAAAGGTTAAAAGCCAAATTGTTTGGTGCTGGTGATGATCATTGTTATAAGATATTAACAGCCCCTAGCAGGACACAA
TTCAAGGTAGTTGAACTATTTGCTGGTGCTGGTGGTTTGGCGCTTGGTTTCCATAATGCTGGTTTAGATTGTTCAATGTT
GGTTGAAATTGATAAGAACCCAGTCGAAACGTTAAGGCATAATTGCCCATCATGGAATGTTATTCATGATGATATTGCAA
ATGTTGACTTCCAAGGAATTACTGCTGATGTAGTTGCAGGAGGATTTCCCTGCCAGGCATTCAGCTATGCTGGGAAAAAA
CTTGGTTTTGAAGACACCAGAGGAACCTTATTTTTTGAATACGCTAGAGCGATTAAAGAAATAAACCCTAAAGTAATTGT
CGGTGAAAATGTTCGTGGATTGGAAAAACATGACAGTGGCCGGACATTAAATACTATGCTGCAAATTTTAGATCAATTAG
GTTATGACGTTGAATACAAAATCCTCCGTGCCCAGTACTTAGATGTGCCTCAAAAACGCGAACGCCTTGTTATGCTGGGC
ATCAGGAAAGATCTTAAAGGCACTATCGCATTTCCCAAAGAACAGGATTATACAATTAGCCTAAGAGAGGCTTTAAAAGA
TGTACCTGATGCTCCTGGCCAAAAATATACTGAGAAAAAATCTAAGGTAATGTCGCTAGTTCCAGAGGGTGGCTATTGGA
GAGATTTACCAGATGACAAACAAAAGGAATACATGGGAGCCAGTTATTTTTTGGGTGGAGGAAAAACAGGAATGGCCAGG
CGTTTGGCATGGGATGAACCATCCTTAACCCTAACGTGCAACCCTGCCCAAAAACAGACTGAACGTTGCCATCCTAAAGA
AACTAGGCCATTAAATGTTAGGGAATATGCAAGGATCCAAACCTTTCCTGATTCATGGATGTTCTCTGGCACAGTATCAT
CGCAGTATAAACAAATCGGTAATGCTGTGCCTGTAAACTTGGGCTACCATATTGGCAGGTGTGTTATTGCTATGCTTACC
CAGAGGTTTGATAGGAAAGATCTAATTATTTGCTCCCGAAAGCCTGATTAA

Upstream 100 bases:

>100_bases
TCCTCAAAAAAATTATATTTGATCCAAATTAACTGTAACCTTTACATAGGCAATGGTTATATCTATTATATTGATATAAG
AATAAAAATAAGGAAAAACA

Downstream 100 bases:

>100_bases
AATATTTCATAACACTATGCAATTCAGCAAGATTTATATCAGGCAGAATTTTTTGAATCACTAAAGGTAATGCTTGATAA
ATGGCTTCAAGTGCATTCTG

Product: modification methylase (Eco47II, Sau96I)

Products: NA

Alternate protein names: M.Eco47II; Cytosine-specific methyltransferase Eco47II [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDTGHRLFQITELQRLKAKLFGAGDDHCYKILTAPSRTQ
FKVVELFAGAGGLALGFHNAGLDCSMLVEIDKNPVETLRHNCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKK
LGFEDTRGTLFFEYARAIKEINPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVEYKILRAQYLDVPQKRERLVMLG
IRKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKYTEKKSKVMSLVPEGGYWRDLPDDKQKEYMGASYFLGGGKTGMAR
RLAWDEPSLTLTCNPAQKQTERCHPKETRPLNVREYARIQTFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIGRCVIAMLT
QRFDRKDLIICSRKPD

Sequences:

>Translated_416_residues
MSYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDTGHRLFQITELQRLKAKLFGAGDDHCYKILTAPSRTQ
FKVVELFAGAGGLALGFHNAGLDCSMLVEIDKNPVETLRHNCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKK
LGFEDTRGTLFFEYARAIKEINPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVEYKILRAQYLDVPQKRERLVMLG
IRKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKYTEKKSKVMSLVPEGGYWRDLPDDKQKEYMGASYFLGGGKTGMAR
RLAWDEPSLTLTCNPAQKQTERCHPKETRPLNVREYARIQTFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIGRCVIAMLT
QRFDRKDLIICSRKPD
>Mature_415_residues
SYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDTGHRLFQITELQRLKAKLFGAGDDHCYKILTAPSRTQF
KVVELFAGAGGLALGFHNAGLDCSMLVEIDKNPVETLRHNCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKKL
GFEDTRGTLFFEYARAIKEINPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVEYKILRAQYLDVPQKRERLVMLGI
RKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKYTEKKSKVMSLVPEGGYWRDLPDDKQKEYMGASYFLGGGKTGMARR
LAWDEPSLTLTCNPAQKQTERCHPKETRPLNVREYARIQTFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIGRCVIAMLTQ
RFDRKDLIICSRKPD

Specific function: This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=426, Percent_Identity=28.169014084507, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR009061
- InterPro:   IPR000551 [H]

Pfam domain/function: PF00145 DNA_methylase; PF00376 MerR [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 46835; Mature: 46704

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDTGHRLFQITELQRLKAK
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEHHHHHHHHHHH
LFGAGDDHCYKILTAPSRTQFKVVELFAGAGGLALGFHNAGLDCSMLVEIDKNPVETLRH
HCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHC
NCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKKLGFEDTRGTLFFEYARAIKE
CCCCCCEEHHHHCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
INPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVEYKILRAQYLDVPQKRERLVMLG
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCHHHCCEEEEE
IRKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKYTEKKSKVMSLVPEGGYWRDLPDDK
ECCCCCCCEECCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
QKEYMGASYFLGGGKTGMARRLAWDEPSLTLTCNPAQKQTERCHPKETRPLNVREYARIQ
HHHHCCCEEEECCCCCCHHHHEECCCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHEE
TFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIGRCVIAMLTQRFDRKDLIICSRKPD
ECCCCCEECCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure 
SYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDTGHRLFQITELQRLKAK
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEHHHHHHHHHHH
LFGAGDDHCYKILTAPSRTQFKVVELFAGAGGLALGFHNAGLDCSMLVEIDKNPVETLRH
HCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHC
NCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKKLGFEDTRGTLFFEYARAIKE
CCCCCCEEHHHHCCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
INPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVEYKILRAQYLDVPQKRERLVMLG
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCHHHCCEEEEE
IRKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKYTEKKSKVMSLVPEGGYWRDLPDDK
ECCCCCCCEECCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
QKEYMGASYFLGGGKTGMARRLAWDEPSLTLTCNPAQKQTERCHPKETRPLNVREYARIQ
HHHHCCCEEEECCCCCCHHHHEECCCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHEE
TFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIGRCVIAMLTQRFDRKDLIICSRKPD
ECCCCCEECCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607524 [H]