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The map label for this gene is flgJ

Identifier: 52841456

GI number: 52841456

Start: 1349296

End: 1350180

Strand: Direct

Name: flgJ

Synonym: lpg1224

Alternate gene names: 52841456

Gene position: 1349296-1350180 (Clockwise)

Preceding gene: 52841455

Following gene: 52841457

Centisome position: 39.71

GC content: 38.31

Gene sequence:

>885_bases
GTGATGGATATGACCATACAAAGTATTGCTACGAGTGATTTTCAAGGGTTAAATGAATTAAAAATTCAGGCAAAAAACAA
TGCAAAAGAAGCGTTGCCCGAGGTAGCTAAACAGTTTGAAGGAATATTCTTGCAATCCATGTTAAAAAGCATGAGGATGG
GGCAACATTTCCTTGATGAATCGAGTCCTTTTAGTGGTAAGAATGAAGCAACCTTTCAAGAGATGCTGGATGCACAATAT
GCTAGTACTATTGCCGAGTCTAAAGGGATAGGATTGGCAGCTCTGCTGGCTAAACAACTGGAAAATTCAGTTGGGGATAA
AGCAAATAAACCAGTTAATTCTTCTACAGAGGTCAACAATACTAAAGTGACTAATTCAGAAGAATCGTTATCTGTTGTTG
ATGATTTCGTAAAATCTGTGTGGCCCACAGCAAAACAGGCTGCATCATTAATTGGATTGGATCCCAAGCTGTTGGTAGCG
CAAGCGGCATTGGAGACTGGCTGGGGTAGATTTGTTACAAGAGATGCAGATGGTTCAAGCAGTAATAATTTATTTAATAT
AAAAACCGGGTCGCATAGCGAAGTAGAGTCAATTCAGGTTAAAACAACAGAATACATTGCCGATACACCCATTAAGATCA
ATGCTTCCTTTAGAAAATATCCATCCATAGAGCATAGTTTTCATGACTATGTTTCATTAATTAAGGGAAGTGAGCGATAC
CAGATGGCCTTGGCCAATGCAGAAAATCCTGAGATTTTTGTCAGTGAGCTTAATAAGGCAGGGTATGCTACTGACCCAAA
TTATAGTAATAAGATTTTGTCTATTTATCATGGTGATGAATTGAACCAGGCAATACAACGATGTGAATCATTCGAACAAA
GTTAA

Upstream 100 bases:

>100_bases
GGATCAATGCTGTTGGTGCAACACCTGCTGATGTGATTTCCATACTGGAAGCTTTACAACAGGCGGGCGCATTGAGTGCT
ACATTAATAGTCATATAAAG

Downstream 100 bases:

>100_bases
TAGTTAGGCCCAAGTTAAAGAAAAAATTAGTTCAGGGAGTGAACAAAATATGTCTATACTTAATATTGCCTATTCAGGAT
TGAATGCTTTTCAGCGTGCC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MMDMTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGQHFLDESSPFSGKNEATFQEMLDAQY
ASTIAESKGIGLAALLAKQLENSVGDKANKPVNSSTEVNNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVA
QAALETGWGRFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYVSLIKGSERY
QMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESFEQS

Sequences:

>Translated_294_residues
MMDMTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGQHFLDESSPFSGKNEATFQEMLDAQY
ASTIAESKGIGLAALLAKQLENSVGDKANKPVNSSTEVNNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVA
QAALETGWGRFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYVSLIKGSERY
QMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESFEQS
>Mature_294_residues
MMDMTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGQHFLDESSPFSGKNEATFQEMLDAQY
ASTIAESKGIGLAALLAKQLENSVGDKANKPVNSSTEVNNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVA
QAALETGWGRFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYVSLIKGSERY
QMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESFEQS

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=291, Percent_Identity=31.9587628865979, Blast_Score=130, Evalue=9e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 32355; Mature: 32355

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDMTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGQHFLDE
CCCCHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSPFSGKNEATFQEMLDAQYASTIAESKGIGLAALLAKQLENSVGDKANKPVNSSTEVNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVAQAALETGWGRFVTRDADGSS
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCC
SNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYVSLIKGSERY
CCCEEEEECCCCCCHHHEEEEHHHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCHHH
QMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESFEQS
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMDMTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSMLKSMRMGQHFLDE
CCCCHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSPFSGKNEATFQEMLDAQYASTIAESKGIGLAALLAKQLENSVGDKANKPVNSSTEVNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVAQAALETGWGRFVTRDADGSS
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCC
SNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYPSIEHSFHDYVSLIKGSERY
CCCEEEEECCCCCCHHHEEEEHHHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCHHH
QMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGDELNQAIQRCESFEQS
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]