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The map label for this gene is artJ [H]

Identifier: 52841359

GI number: 52841359

Start: 1236120

End: 1236878

Strand: Direct

Name: artJ [H]

Synonym: lpg1125

Alternate gene names: 52841359

Gene position: 1236120-1236878 (Clockwise)

Preceding gene: 52841356

Following gene: 52841360

Centisome position: 36.38

GC content: 34.52

Gene sequence:

>759_bases
TTGGCTGAATTTTTTCTGATTAATCTATACTTAGTGAATCGATTCCAAACAATAGTTATATCTATGATTCTAAAGCCGGG
AAAATTATTGGTGGCTACTGCTTATCCAGATCCACCGTTTGATATTGAGCCAGGTGATGGTTTTGATGGTGATTTAATAA
AAGAAATTTGTCGAATTTTAAATTTGCAGCTAGTCCAGATCAAATATGAGGGAGAAAATTTTAATGATATTTTTTTAGGC
TTGGCTAATAAAAAATACGATGTTGTCATTTCAGGAACGACTATTACACCAAACCGCTCAAAACAAGTACTTTTTTCGAA
TCCCTATTTACAGTTTAATCAGGGTATCGCGGTTAATACTGAATTAAAGCCAAATGTTTCTTCTTTTGAGGATTTGACAG
GTTTGGTGGCTGGTATACAGAAAGGCAATACCTCGGACGAAGTTGCTCAGGATTTATTACGTAAGAACATACTCAGTGAT
ATTAAATATTATGCATACAATGAAATAGAATTAGCCATACAAGATCTGTCTGCCGGTAAAATTGGTTTGGTCATTAAATT
ATATCCTGTTATTTCCTGTTTAATTCGAAACTATACAAATTTGGCTGTTCCTTTGCAGGCTGAAACACATGAAAAACTTG
GTATCGCGTTTGCTAAAGATAATTTTAAACTGCGGGATAAAGTCAATATTGTCCTAGCCGATCTTGTGCAAAATGGCACT
TTAAGTCGCTTGCAACATAAGTGGATAAGTACAACCTAG

Upstream 100 bases:

>100_bases
TTTTTATAATTTTAGCTGTTAATTACATAACCAATCCTGGATATATTACTCGTGTAATCATGGGTAAATTATTCATTTAA
ATCAATGGCATAAAAAATAT

Downstream 100 bases:

>100_bases
GGTAAATTAGGCCAATGTCAGTTTCAACTCAGGAGATAACCTTATTTAAAGACAGGACTTACGATAAACTCCAATCTCTT
TGTTAAAAAAAGTTTTTAAC

Product: cystine/glutamine-binding periplasmic protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MAEFFLINLYLVNRFQTIVISMILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLG
LANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNTELKPNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSD
IKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNGT
LSRLQHKWISTT

Sequences:

>Translated_252_residues
MAEFFLINLYLVNRFQTIVISMILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLG
LANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNTELKPNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSD
IKYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNGT
LSRLQHKWISTT
>Mature_251_residues
AEFFLINLYLVNRFQTIVISMILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
ANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNTELKPNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSDI
KYYAYNEIELAIQDLSAGKIGLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNGTL
SRLQHKWISTT

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787031, Length=232, Percent_Identity=31.8965517241379, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787088, Length=227, Percent_Identity=27.7533039647577, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=26.1603375527426, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28228; Mature: 28097

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFFLINLYLVNRFQTIVISMILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRIL
CCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
NLQLVQIKYEGENFNDIFLGLANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNT
CEEEEEEEECCCCCCEEEEEECCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEEC
ELKPNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGK
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEEECCCCC
IGLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNGT
EEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCEEEEHHHHEEEEHHHCCCC
LSRLQHKWISTT
HHHHHHHHCCCC
>Mature Secondary Structure 
AEFFLINLYLVNRFQTIVISMILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRIL
CCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
NLQLVQIKYEGENFNDIFLGLANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNT
CEEEEEEEECCCCCCEEEEEECCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEEC
ELKPNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGK
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEEEEECCCCC
IGLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNGT
EEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCEEEEHHHHEEEEHHHCCCC
LSRLQHKWISTT
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]