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The map label for this gene is murE

Identifier: 52841152

GI number: 52841152

Start: 990018

End: 991469

Strand: Direct

Name: murE

Synonym: lpg0917

Alternate gene names: 52841152

Gene position: 990018-991469 (Clockwise)

Preceding gene: 52841151

Following gene: 52841155

Centisome position: 29.14

GC content: 41.12

Gene sequence:

>1452_bases
GTGAAATTAACCCAATTATTAGAACCGTGGATTAAAAGGGACATTATTGATGGCACTGTGAGTGACATAAAAAATGACAG
CCGCTTGGTGAAAGAAGGGGATTTATTTGTCGCCTATCCAGGGGCAGCTTCCGATGGTCGATTATATATAGAAAAAGCCG
TGGCATCAGGTGCTGTCGCTGTGGTTTATGAGCCCAATAATTTACCTCAGGGAATTACTTTGCCAACAGGAATTCCCTGT
ATAGCCATTCCCCAATTAGCTGAAAAATTAGGGGAAATAGCGAAACGGTTTTTTAATAACCCATCCCAATTTCTTGGTTT
AACTGGGGTAACTGGCACAAATGGTAAGACGACTATTGCTTATCAGTTGGCACAGGCACATGATTTACTCGGGCAGCACT
CGGCTTACATCGGGACTATAGGGCAAGGAAAGGTGGATGCCTTAAAACCTTTGGAAAATACTACGCCAGATGCTCTTTGT
TTGCAGAGGTTAATGCATCAGTATAAAGAAGGAGGCATTAAACAGGTTTGTATGGAGGTATCCTCTCATGCGCTGAGCCA
GCATAGAGTCGACTCTCTCGAATTTAGTCAGGCTATCTTTACCAATTTAACGCTCGATCATTTAGATTATCACCAGACAA
TGCAAGCTTATGGGGCTGCAAAGTCGAGGTTGTTTGAACGAGCAGAGTTGCAATGGGCTATTATTAATCAGGATGATGAC
TTTCAAGATCTCATGAGCAATGTGCTAAAGACTCATGTAAAAAAAGTGACTTACGGTATGCATCAAAATTGCGATGTGAA
AGCGCTCCAATGGAATATGGATATCACCGGAACAGAAATTGAAGTCAAATCTCCTTGGGGGCACCACCTTCTAAAGATTA
AAGCACTGGGTAAGTTTAATATTTATAATAGCCTGGCTGTTTATAGCAGTTTGCTTGCCAGCGGGTATAATCCAGAAAAA
GTAGTGCAAGTTATGGCCGAATTAAAAGCGGCTCCTGGCCGAATGGAAATTGTCGCTCAATCCCCATATGTTCTTGTTGA
TTATGCTCATACTCCCGATGCATTGGAAAATGTTCTTATCACCTTAAAACAAATAAAAAAAGGGAGATTGTGGGTTGTAT
TTGGTTGTGGTGGGGACAGAGATAAAACCAAACGGCCCATTATGGGAAAGGCAGCCAGCATGTACGCTGATAATATTGTC
ATTACCAGCGATAATCCAAGAAGTGAAGATCCGGAAATTATTCTAAATGAAATTGCTTGTGGCATTCCTGCCTCTGCGCC
TGTCTCTAAACTGGTGAATAGAGAAGAGGCAATTGCTTATGCTTTAAGAAAGGCTGAGCATAACGATATCATATTAATAG
CAGGAAAAGGACATGAATCGTATCAGCAGATTGGGAATACAAAACATGTTTTTTCTGATCAGGAAGTTGTAAGACGTTTG
ATGAATAATTAA

Upstream 100 bases:

>100_bases
TGGCAGGGCCCTTATTTGCGAAAGTCATGTCAGGTGCTTTACGCTTATTCAATATTCCTACTGATGAGCCAGTTAGTTAA
AGAGAATAGGAGCCAATTTT

Downstream 100 bases:

>100_bases
AATTTCGGGACAGGGGTTAATTATTAGAAACAGACATCAACACCTTAACCTAATAATGGATACTTTTTTATTGAGTCAGA
ATCAAAATAAGTTGTGAAAT

Product: UDP-N-acetylmuramyl-tripeptide synthetase MurE

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAASDGRLYIEKAVASGAVAVVYEPNNLPQGITLPTGIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEGGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERAELQWAIINQDDD
FQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQVMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN

Sequences:

>Translated_483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAASDGRLYIEKAVASGAVAVVYEPNNLPQGITLPTGIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEGGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERAELQWAIINQDDD
FQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQVMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN
>Mature_483_residues
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAASDGRLYIEKAVASGAVAVVYEPNNLPQGITLPTGIPC
IAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
LQRLMHQYKEGGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERAELQWAIINQDDD
FQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDITGTEIEVKSPWGHHLLKIKALGKFNIYNSLAVYSSLLASGYNPEK
VVQVMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIV
ITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
MNN

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=41.3441955193483, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53376; Mature: 53376

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAASDGRLYIEKAVASGAVA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCEEEEECCCCCCCEEEEEEHHCCCCEE
VVYEPNNLPQGITLPTGIPCIAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIA
EEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCCEEEE
YQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALCLQRLMHQYKEGGIKQVCMEV
EHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
SSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERAELQWAIINQDDD
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
FQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDITGTEIEVKSPWGHHLLKIKALGKFN
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
IYNSLAVYSSLLASGYNPEKVVQVMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHH
TLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIVITSDNPRSEDPEIILNEIAC
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHC
GIPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHH
MNN
HCC
>Mature Secondary Structure
MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAASDGRLYIEKAVASGAVA
CCHHHHHHHHHHHHHCCCHHHHCCCCCCEEECCCEEEEECCCCCCCEEEEEEHHCCCCEE
VVYEPNNLPQGITLPTGIPCIAIPQLAEKLGEIAKRFFNNPSQFLGLTGVTGTNGKTTIA
EEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCCEEEE
YQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALCLQRLMHQYKEGGIKQVCMEV
EHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
SSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYGAAKSRLFERAELQWAIINQDDD
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
FQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDITGTEIEVKSPWGHHLLKIKALGKFN
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
IYNSLAVYSSLLASGYNPEKVVQVMAELKAAPGRMEIVAQSPYVLVDYAHTPDALENVLI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHH
TLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAASMYADNIVITSDNPRSEDPEIILNEIAC
HHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHC
GIPASAPVSKLVNREEAIAYALRKAEHNDIILIAGKGHESYQQIGNTKHVFSDQEVVRRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHH
MNN
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]