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The map label for this gene is mlaE [H]

Identifier: 52841078

GI number: 52841078

Start: 920347

End: 921129

Strand: Direct

Name: mlaE [H]

Synonym: lpg0842

Alternate gene names: 52841078

Gene position: 920347-921129 (Clockwise)

Preceding gene: 52841077

Following gene: 52841079

Centisome position: 27.09

GC content: 39.59

Gene sequence:

>783_bases
ATGCTTGATACAATCGCGCGTCTGGGTAATCGAGGTGTACAAGCCTTACAAGGTATTGGTAGCTCAGGGCTGTTTTTATT
TTCTATGTTGTTCAGAAAGCCTAATGTTTTAAGGTTATGGCCATTATTACGTTATCAGCTTTATTTTATCGGTGTATTGT
CTTGTTTAATTATTGTGGTTTCAGCCTTGTTTATTGGTATGGTTGTAGGACTTCAGGGTTATAATACGTTACAAAAATTT
GGTGCTTCAAGTCAATTAGGCCAATTATTGGCTTTAAGCGTCGCCAGAGAATTAGGACCTGTGATTAGCGCTTTGTTGTT
TGCAGGGCGTGCCGGGTCAGCCCTTACAGCTGAAATTGGTTTAATGAAGGCAACAGAGCAGTTATCCAGTATGGATATGA
TGGGAGTGGATCCATTAGGAAGAGTGGTTTATCCGCGTTTTGTGGCAGGATTTATAGCGTTACCGATACTTGCTTTGATT
TTTTCTGCAGTAGCTATATATGGTGGTTATTTTATTGGTGTTTACTGGCTAGGCGTGGATGCAGGGAGTTTTTGGTCTAA
TATGCAAGCTGCTGTAAATTTTCGTATAGATATCTTAAGCGGGATTATTAAAAGCTTGGTTTTCGCATTTGTGGTTACTT
GGATTTCTGTATACCAAGGTTTTGAGTGTGTGCCAACAGCTGAAGGCATTAGTCATGCTACAACTAAAACTGTAGTATAC
TCATCGCTTGCTGTATTAGGTCTTGATTTCTTGTTGACTGCAATGATGATTGGAGATTGGTAA

Upstream 100 bases:

>100_bases
CGATCTATTGAGCCTCAGATAAGACAGTTTATGCATGGAGAAGCGGATGGGGTAGTGCCTTTCCATTATCCGGCAAAACC
GTATACAGAGGAGTTATTAA

Downstream 100 bases:

>100_bases
ATGAAAAAGCAACGTTATGTAGACATTAGTGTAGGAATATTTATGTTGCTTGGGCTGCTAGCATTATTAGTAATGGCGAT
GAAAGTGAGTAACATTACTG

Product: toluene tolerance protein Ttg2B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW

Sequences:

>Translated_260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW
>Mature_260_residues
MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLRYQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKF
GASSQLGQLLALSVARELGPVISALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVYQGFECVPTAEGISHATTKTVVY
SSLAVLGLDFLLTAMMIGDW

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=61.003861003861, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28017; Mature: 28017

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]