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The map label for this gene is hisF

Identifier: 52840986

GI number: 52840986

Start: 820735

End: 821499

Strand: Direct

Name: hisF

Synonym: lpg0749

Alternate gene names: 52840986

Gene position: 820735-821499 (Clockwise)

Preceding gene: 52840985

Following gene: 52840987

Centisome position: 24.16

GC content: 40.52

Gene sequence:

>765_bases
TTGATGAGTAATGTCCGTCTAATTGCACGACTAGATATTAAAGGACCTAACCTAATCAAAGGAGTGCATCTGGAGGGATT
AAGAGTAGTAGGAAATCCCAATGAATATGCTATGGCATATTATGCACAAGGTGCAGATGAATTGATTTATATGGATACAG
TTGCATCTTTGTATGGGCGTAATAATTTGTCAGAAATTGTAAAGACTACTGCTGAAAATGTATTTATCCCAATTACTGTA
GGTGGCGGAATTCGCAGTGTTGATGATGCCAAGCAATTATTGCGTTGCGGTGCCGATAAGGTAGCTATTAATACAGCGGC
AACTAAAAACCCTACTCTAATATCTGATATTGCCAGAAGATTTGGGTCTCAATGTGTTGTTTTATCTATCGAAGCAAAAC
GCACTGTGAATGGAAGATGGGAGGTCATGACAGACAATGGACGTGAACATACCGGAATGGATGTTGTTGATTGGGCTAGG
AATGGCGAAGAATTTGGTGCAGGTGAAATTCTTCTGACCTCTATTGATCAGGAAGGTACGCGTAAGGGATTTGATCTGGA
ATTAGTGAAACAGGTGTCTTCCATGGTTTCTATCCCGGTGATTGCAAGTGGGGGTATGGGTAAGCTGGAAGATTTAACTG
AAGTGGTTTGTGAGTCTAAGGCTGATGCAGTCGCTATGGCGGATGTTTTGCATTACAAAAGAATAGGTTTACCTGAAATC
AGAAAGTATGCTTTAGATAATGGCATTCATGTGAGAGTCTTATGA

Upstream 100 bases:

>100_bases
CTCCTCACTTGTGGAAAAAAGATGGAGAACAGTGGAAACTTAGACATCAAGTAACAAATCTTGAAAAAACTAAAGCTGAA
TATCTGGATTTGGAGACTGT

Downstream 100 bases:

>100_bases
GTTCAGTTTCAATTGTAGATTATGGGGTAGGCAATTTATTAAGTGTGGCTCGAGCTTTTCAATATTTTGATGCTTCTGTC
AATTTAGTGAGCACACCCGA

Product: imidazole glycerol phosphate synthase, cyclase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MMSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITV
GGGIRSVDDAKQLLRCGADKVAINTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWAR
NGEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEI
RKYALDNGIHVRVL

Sequences:

>Translated_254_residues
MMSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITV
GGGIRSVDDAKQLLRCGADKVAINTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWAR
NGEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEI
RKYALDNGIHVRVL
>Mature_254_residues
MMSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITV
GGGIRSVDDAKQLLRCGADKVAINTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWAR
NGEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEI
RKYALDNGIHVRVL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=253, Percent_Identity=36.3636363636364, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082028, Length=183, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=279, Percent_Identity=32.9749103942652, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27665; Mature: 27665

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGR
CCCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCC
NNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAINTAATKNPTLISDIARR
CCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHH
FGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGT
HCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCC
RKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEI
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
RKYALDNGIHVRVL
HHHHHCCCEEEEEC
>Mature Secondary Structure
MMSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGR
CCCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCC
NNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAINTAATKNPTLISDIARR
CCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHH
FGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGT
HCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCC
RKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEI
CCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
RKYALDNGIHVRVL
HHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]