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The map label for this gene is slt [H]

Identifier: 52840900

GI number: 52840900

Start: 713454

End: 715274

Strand: Reverse

Name: slt [H]

Synonym: lpg0663

Alternate gene names: 52840900

Gene position: 715274-713454 (Counterclockwise)

Preceding gene: 52840901

Following gene: 52840894

Centisome position: 21.05

GC content: 37.18

Gene sequence:

>1821_bases
GTGCCAAATAATTGCCCTCAAGCCTTATTAAGAGGAATTATGAAAAGATTATTAATTTTTTTATGTGGGCTTCTCTGTTC
TTTCAACTTGTTTGCTTTATCTGGACAAGCCTACATGGACAGATTTAATCGCTATCTCGCATGGTATGAACACCTGCCCA
TTAACCCTTCACCAGAATTTTTGGAATTTGTAAAAGAAAGCACGCCGCTTGCTAATAAATTACGAGATAAATGGCTATAT
GAACTGGCCAGGATAAAGGATTGGGCAAATTATAATAAGTATTATCAACCCACAAACGACATAAACCTCAGATGCTTTGC
GCAAATTGCCAAATTTAATTTAGGATTATACAAGGAGGCTATTGCAGGCTCTATTCCAATCTGGTTAACAGGAGAATCCA
GACCACAAGCATGCAATACCCTATTTGCTTTATTGTTAAAACAGGATAATTTTGATCAGAAATTCATTACGCAACGCATA
ACACTTGCACTAGACAAACGTAATGTTCTCCTCGCTCGTTACCTGTTAAAACAATACAAAACACCTCACGATACAGAAAT
AAAAAACTTAACCAGTGTTTATGAGAATCCTTCCAATATTTCCAAGTTGAACCCTGGCAAATTGAACGATTATTTCTATC
TTTATGGATTAAAAAGATTAGTATCCATTAACATGGATAAAGCAGTGAAATTATGGCAAGAGAAAAAAACCAACATCATG
TTAAATGAGCGACAAAAACAAGATTTTCTTGCACATTTGGCATTATACAAGGCCATGAGAAATCATGCCGATGCTCAGGA
ATGGTTTGCCAAAATCAAACCTGAATACTACACCGATGTGTTAGTCGACTGGCAAATCCGCTTTGCTCTGAAAAATAAAA
ACTGGAAACAAGTTGAGAAATTAATTAACAACTCACCAAATAAAGAGATGCCTTGTTGGCAATATTGGTTGGCTCGCTCC
CTGGAAGAACAAGGGAAAAAAGCAGAGGCGATTAAAATTTATGAGCCACTAGCTAAAAACAGACATTATTATGGTTTTCT
AGCCAGCAAACGCCTGAAAAAAAGACCCAGCTTTGACAATGAAAAACCAACAACCAACACAGAGATTCTCAAACCCTATC
AACCTGTCATAGAGCAAATTAAAAGTTTTTACAACTCCAAACAGCTACTGCAGGCATCAAGGTTATTAAATGACTTCATT
AGTGAATTACCCAAAGAAGAAGCAAGTGCTCTGGTCTATTGGATAGCAACCGAACTAAAATGGCACGGAAAATCAGTTTA
CTTAAGCAATAATGACACACTCAATAATCAATTAGCATTAAGATTTCCATTGGCCTACAAAGACACAATAAAGGCATATG
CATCAAAATACGCCATACCGCCCGAACTTGTATATGCTATTATTCGTCAGGAAAGTGGTTTTAGAGAAGATGTAACATCC
TCTGCTGGAGCTAAAGGGCTAATGCAAGTCATGCCACAAACAGCCAGCGCTGTTTCCAAAACCAGCAAAATACCGTATTC
GGATCATAAACAATTGTTTTTATCTCAAAAGAATATCAATATAGGGATTGCTTATTTACAGCACTTAACCAAGCGCTTCA
GTAACCACCCGGTTCTTGTTGCTGCCGCTTACAACGCCGGTCCCAGGCAAGTAGTCTATTGGCTTAAAAATCACCCACCC
AAAGAAATAGATATCTGGATTGAAACACTTCCCTGGCAAGAAACACGAAATTATCTAAAAAATGTAATGGCCTTTTATAT
TGTTTATCAATACCGTTTAAACCAACAGCCTGATATGAATAGGTTTCTAACACCTTTGTAA

Upstream 100 bases:

>100_bases
CCGGAGCTAACCAATTTGTTGCAGGTTCTGCTATATTCAATAGCAGTAATTATAGTGAAACCATTAAACGTATGCGTGAA
CAATTAGCAAGCATTTAAAA

Downstream 100 bases:

>100_bases
GAAATATTTTCATGGTAGTCAACGTAAATCGTTCTATAGCCAGATCTACGTTTGCGAGCTACCTGTCTGACTTTTGCTAA
TTTAACGTAATGGACAAGGA

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MPNNCPQALLRGIMKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPSPEFLEFVKESTPLANKLRDKWLY
ELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRI
TLALDKRNVLLARYLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIM
LNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARS
LEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQIKSFYNSKQLLQASRLLNDFI
SELPKEEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKAYASKYAIPPELVYAIIRQESGFREDVTS
SAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPP
KEIDIWIETLPWQETRNYLKNVMAFYIVYQYRLNQQPDMNRFLTPL

Sequences:

>Translated_606_residues
MPNNCPQALLRGIMKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPSPEFLEFVKESTPLANKLRDKWLY
ELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRI
TLALDKRNVLLARYLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIM
LNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARS
LEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQIKSFYNSKQLLQASRLLNDFI
SELPKEEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKAYASKYAIPPELVYAIIRQESGFREDVTS
SAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPP
KEIDIWIETLPWQETRNYLKNVMAFYIVYQYRLNQQPDMNRFLTPL
>Mature_605_residues
PNNCPQALLRGIMKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPSPEFLEFVKESTPLANKLRDKWLYE
LARIKDWANYNKYYQPTNDINLRCFAQIAKFNLGLYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRIT
LALDKRNVLLARYLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIML
NERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEKLINNSPNKEMPCWQYWLARSL
EEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQIKSFYNSKQLLQASRLLNDFIS
ELPKEEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKAYASKYAIPPELVYAIIRQESGFREDVTSS
AGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPK
EIDIWIETLPWQETRNYLKNVMAFYIVYQYRLNQQPDMNRFLTPL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=560, Percent_Identity=29.8214285714286, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 71065; Mature: 70934

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNCPQALLRGIMKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPSPEF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHHHCCCCCCCHHH
LEFVKESTPLANKLRDKWLYELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLGLYKEA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRITLALDKRNVLLARYLLKQYK
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHCC
TPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIM
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
LNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEK
ECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHEEEEEEEEEEECCCCHHHHHH
LINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDN
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCC
EKPTTNTEILKPYQPVIEQIKSFYNSKQLLQASRLLNDFISELPKEEASALVYWIATELK
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEHHHE
WHGKSVYLSNNDTLNNQLALRFPLAYKDTIKAYASKYAIPPELVYAIIRQESGFREDVTS
ECCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
SAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEE
AAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYLKNVMAFYIVYQYRLNQQPDMN
EEECCCCCHHEEEHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RFLTPL
HCCCCC
>Mature Secondary Structure 
PNNCPQALLRGIMKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEHLPINPSPEF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHHHCCCCCCCHHH
LEFVKESTPLANKLRDKWLYELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLGLYKEA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRITLALDKRNVLLARYLLKQYK
HCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHCC
TPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKLWQEKKTNIM
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEE
LNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFALKNKNWKQVEK
ECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHEEEEEEEEEEECCCCHHHHHH
LINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFLASKRLKKRPSFDN
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCC
EKPTTNTEILKPYQPVIEQIKSFYNSKQLLQASRLLNDFISELPKEEASALVYWIATELK
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEEHHHE
WHGKSVYLSNNDTLNNQLALRFPLAYKDTIKAYASKYAIPPELVYAIIRQESGFREDVTS
ECCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
SAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINIGIAYLQHLTKRFSNHPVLV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEE
AAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYLKNVMAFYIVYQYRLNQQPDMN
EEECCCCCHHEEEHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RFLTPL
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]