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The map label for this gene is def2 [H]

Identifier: 52840852

GI number: 52840852

Start: 647840

End: 648553

Strand: Reverse

Name: def2 [H]

Synonym: lpg0615

Alternate gene names: 52840852

Gene position: 648553-647840 (Counterclockwise)

Preceding gene: 52840855

Following gene: 52840850

Centisome position: 19.09

GC content: 35.99

Gene sequence:

>714_bases
TTGGGAAAATCAATAATAGTGTATTTGCTACTAACCACATTAACGAGAATCAAAAAGAAATCTCAACAACTTCTTATTTT
AATAGGCTTCTTATTAGGGATGTCACCAGTCATGTCTTTAGAAATTATTACTATAGAAAAAATAGAAAACCACCATATGC
TAAAAACTCGGGCTGAAAATATTCAATTTCCCTTGAGTCGAGAAGATAAAGATTTAATAGAAGCGATGAAAAAAAAATTA
TACGCATTGGGAGGGGTTGGTTTGGCAGCACCACAAGTCAATTCCGCCAAACAAATTATCGCAGTTTACATCCCTGAAGA
AGCTCAGCTTTTACGTGATAATGCGAAAATCTATCCAATGCACATCCTAATCAACCCAAATTACGAGCCCTTGCCCCAGG
CTCGTATTATTAGCGACTTTGAAGGTTGTTATTCTGTATCCAGTAAAGCAGGGAAAGTGCCAAGGTATGATGAAATTCAG
CTAAAGTACTATGATGAAGAAGGGAATGTTCACCAACAAATCGAAAATGGCTTTTACGCCCGTGTTTTGCAACATGAAAT
TGATCATCTGAATGGCGTATTAATCACCGATCGTTTAACTCCCGATTGTGTACAAGGGACTATTGAAGAAATGATGACTA
TTCGCCGTGCTGAATTGCCTTTAGAAAAACGAAAATTATTTGATCAAATCATGGCAAAAAAACTTAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTAATAAGGGAGTTTAGATAAACTAAATAACCGAATTAAAAACTTTTTTAACAAAGAGATTGAGGTTTTTCGTAAGT
CCTGATAGTTTCTTGTCCAA

Downstream 100 bases:

>100_bases
GGTCGAGTTTCCAAATTATCTTTTATGAATTGACTTCAATGATAAACTGCCCTGGGTAATTTTTTTGCTTGTTCAACCCA
GGTAACAACTTGAGATAATC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGKSIIVYLLLTTLTRIKKKSQQLLILIGFLLGMSPVMSLEIITIEKIENHHMLKTRAENIQFPLSREDKDLIEAMKKKL
YALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPMHILINPNYEPLPQARIISDFEGCYSVSSKAGKVPRYDEIQ
LKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK

Sequences:

>Translated_237_residues
MGKSIIVYLLLTTLTRIKKKSQQLLILIGFLLGMSPVMSLEIITIEKIENHHMLKTRAENIQFPLSREDKDLIEAMKKKL
YALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPMHILINPNYEPLPQARIISDFEGCYSVSSKAGKVPRYDEIQ
LKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK
>Mature_236_residues
GKSIIVYLLLTTLTRIKKKSQQLLILIGFLLGMSPVMSLEIITIEKIENHHMLKTRAENIQFPLSREDKDLIEAMKKKLY
ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPMHILINPNYEPLPQARIISDFEGCYSVSSKAGKVPRYDEIQL
KYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=144, Percent_Identity=30.5555555555556, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1789682, Length=130, Percent_Identity=33.0769230769231, Blast_Score=68, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24645726, Length=173, Percent_Identity=32.9479768786127, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=32.3170731707317, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 27145; Mature: 27014

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSIIVYLLLTTLTRIKKKSQQLLILIGFLLGMSPVMSLEIITIEKIENHHMLKTRAEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCHHHHHHHHC
IQFPLSREDKDLIEAMKKKLYALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEE
HILINPNYEPLPQARIISDFEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYA
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCHHH
RVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK
HHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKSIIVYLLLTTLTRIKKKSQQLLILIGFLLGMSPVMSLEIITIEKIENHHMLKTRAEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCHHHHHHHHC
IQFPLSREDKDLIEAMKKKLYALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIYPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEE
HILINPNYEPLPQARIISDFEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYA
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCHHH
RVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRAELPLEKRKLFDQIMAKKLKK
HHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]