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The map label for this gene is lolB [H]

Identifier: 52840790

GI number: 52840790

Start: 586676

End: 587275

Strand: Reverse

Name: lolB [H]

Synonym: lpg0547

Alternate gene names: 52840790

Gene position: 587275-586676 (Counterclockwise)

Preceding gene: 52840793

Following gene: 52840788

Centisome position: 17.28

GC content: 40.83

Gene sequence:

>600_bases
ATGAATGCGATAAAACGTTTGACAATCATTTTAATTTGCTTTTTAACAGCATGCGCTCCCCCAAGACCCTCTGAAGAGCC
GCCTGCAAACCGTGAAATTCCTCTTGAAGAACGTAAAGCCGAAACAGCCACTGTTTCCTCATGGGAAATTAAAGGGGCTC
TGGCTGCAAAAACCAAAGCGAAAGGATGGTCTGCTACTATGAATTGGGTCCAACAAGGGGCCAACTCATACCAAATTCGT
TTAATGGGGCCATTAGGTGGTGGAGCCGTTCTAATCAGCAAAAAGGGGAATACTATTACATTTCAAGATGGCGCCAAAAT
AACTACTTCGACCAATGCCGATGAGTTATTACTAAAACAAACTGGCATTCGACTTCCAGTAAATAATTTGTATTACTGGG
TAAGAGGTTTACCCGCACCAGGTGGAATCCAATCAGAAAAACATGACCAATTCAATCACCTGATACAATTAAAGCAAAAT
GGCTATACCATTGATTTTACTAAGTATACTTCGGTCAAGGGAATAGATTTACCAAGTATGATTCGCCTTGAAGGAAATGG
CGTCATGGTAAAGGTAGTGATAAAGAATTGGGTTGTATAA

Upstream 100 bases:

>100_bases
ATTGCTTTTTGTTTCATCTCGTTAATTACCATTTGCATATAGAAGCAGTCTAGCGTATTGTAAATTGAAAAACTATAAAA
AAATGGAATTGAGGTAAGCA

Downstream 100 bases:

>100_bases
TTTAATTCTTGTAATTTATTTGCTTTCTGAAGTTGACATCGATAAAAAAGCACTGCAAAATACGCAGCACATTGCAGGGA
TGAATACTATTTATAAACTC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETATVSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIR
LMGPLGGGAVLISKKGNTITFQDGAKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQN
GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV

Sequences:

>Translated_199_residues
MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETATVSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIR
LMGPLGGGAVLISKKGNTITFQDGAKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQN
GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV
>Mature_199_residues
MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETATVSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIR
LMGPLGGGAVLISKKGNTITFQDGAKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQN
GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=201, Percent_Identity=25.3731343283582, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 21893; Mature: 21893

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETATVSSWEIKGALAAKTKA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCEEEECCEEECEEEEECCCC
KGWSATMNWVQQGANSYQIRLMGPLGGGAVLISKKGNTITFQDGAKITTSTNADELLLKQ
CCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCCEEEECCCHHHHHHHH
TGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQNGYTIDFTKYTSVKGIDLPSM
CCCCCCHHHEEEEEECCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEEECCCCCCCCCCE
IRLEGNGVMVKVVIKNWVV
EEECCCCEEEEEEECCCCC
>Mature Secondary Structure
MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETATVSSWEIKGALAAKTKA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCEEEECCEEECEEEEECCCC
KGWSATMNWVQQGANSYQIRLMGPLGGGAVLISKKGNTITFQDGAKITTSTNADELLLKQ
CCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCCEEEECCCHHHHHHHH
TGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQNGYTIDFTKYTSVKGIDLPSM
CCCCCCHHHEEEEEECCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEEECCCCCCCCCCE
IRLEGNGVMVKVVIKNWVV
EEECCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA