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The map label for this gene is tphA

Identifier: 52840702

GI number: 52840702

Start: 496446

End: 497702

Strand: Reverse

Name: tphA

Synonym: lpg0457

Alternate gene names: 52840702

Gene position: 497702-496446 (Counterclockwise)

Preceding gene: 52840703

Following gene: 52840684

Centisome position: 14.65

GC content: 39.38

Gene sequence:

>1257_bases
ATGAAACAAAATACCAAACACAGCATTATTGCTGGATTATACGGAAATGCTTTGGAGTGGTATGATTTCTTGCTCTATGC
CAGTTTTGCTCCTGTCTTTGCTGAAATTTTTTTCCCTTCAAAAATCCATTTTGTTTCTCTTATAGCAACATTTAGCTTAT
TTGCTATAGGATTCATTATGCGTCCAATTGGTGGGCTTTTGCTCGGGCACTATGCTGATCATGTAGGACGGCGGAAAGCA
TTAATACTATCTATGTCAGTAATGACCATTTCTACCGCATTAATTGCAGTATTACCTGATTTTAATTCATGGGGAATTAT
CGCCCCACTTTTATTTTCTGTTTTACGGCTCATTCAGGGGCTTGCAGTGGGAGGCGAACTGCCCGGCTCGACCACCTATT
TGATTGAGCATATGTTTCAACAAAGCCGGGGGCTCGCTGGCAGCCTCGTGTTAAGTAGCGCTTTTTTAGGTATTTTTATT
GGAGCGCTTACCGCCTCAATATTCAGCACATTATTTACAGGTGATGCTCTATTAAATTGGGGGTGGAGGTATGCGTATCT
GTTAGGAGGTATTTTAGGAATTTTGGGCATTTATTTGCGTATTAAAAGCTTTGAATCACCCACATTTCTAAAGGATAAGC
ATACTGCTGAACTTCCTGCAAAAGTGGTATTCACGCGATTTAAACAGCAACTGTTTTTATCTGTGATAATTACTAGCATT
CTTGCCATGTCTAATTATGTACTTATCGCCTATGTCACAACTTTTTTAGTCAAATCAGAGTATTTTCTCTTGAAAGATGC
GTTATTAATCAATTTAATTGCCCTTGCTCTTTTGACAGTTTTAATACCGCTGATGGGATTTCTTTCTGATGTTGTGGGAC
GTAAGCCTATTTTCTTAATCGGTTTATTTGCTCTTTGCCTACTAATCTTCCCATTTTTTTGGCTCTTATTAAGTGGTAGT
TGGTGGAAAGCATTATGCAGCGAATTATTACTAGCGATAGCTTTAGCCCCAATCAATGCGACAGTACCCACTATTATTGC
AGAAATGTTTCCAACCAGTGTACGGGCGAGTGGGATTTCCATATCCTATAACATTGGCCAGGCTTTATTTGGCGGTACCA
TGCCTTTGGTTGCGTTTAGCCTGATTGAATTAAGCAGCAATAAAATGGCTCCGGCTTGGTACCTTTTCCTATGGACCCTT
ATCGTGATACCAATAACTATTTCCATTCGTGAAAGCTATCAAAAAGAGCTTACTTAA

Upstream 100 bases:

>100_bases
GCAACTCCGGCCGCTATTTATTAAAAACAAACAACCAAATTAATCCTTTTACTCCAGGAATTCTAAATGGGTTTACTTTA
AACGACCATATTGCATAATT

Downstream 100 bases:

>100_bases
GGCATTAGATTGAGATGAACCGACATAATTTCTATCGATTGAATAAACAACAATAGATTTAAATCAGTTTTTTAGTAGAA
AAAACCCAGCATTAATAGCC

Product: TphA (ProP)

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSVLRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFALCLLIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFSLIELSSNKMAPAWYLFLWTL
IVIPITISIRESYQKELT

Sequences:

>Translated_418_residues
MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSVLRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFALCLLIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFSLIELSSNKMAPAWYLFLWTL
IVIPITISIRESYQKELT
>Mature_418_residues
MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSVLRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFALCLLIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFSLIELSSNKMAPAWYLFLWTL
IVIPITISIRESYQKELT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=30.4245283018868, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=29.5238095238095, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=30.5210918114144, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789941, Length=389, Percent_Identity=32.3907455012853, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082404, Length=326, Percent_Identity=26.0736196319018, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082231, Length=209, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46070; Mature: 46070

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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EEEEHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]