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The map label for this gene is trmD

Identifier: 52840641

GI number: 52840641

Start: 436971

End: 437741

Strand: Reverse

Name: trmD

Synonym: lpg0396

Alternate gene names: 52840641

Gene position: 437741-436971 (Counterclockwise)

Preceding gene: 52840642

Following gene: 52840640

Centisome position: 12.88

GC content: 42.28

Gene sequence:

>771_bases
TTGGGATATGAATTTTTAGTGGCATTACATCTTGGAGTCATCACCCTGTTGCCTGAAATAATACAAGGCATCCATTATGG
GGTAACCGGCCGAGCTATTGAACAAGGCTTGGTTAAAATTGATTGCTGGAATCCTAGAGATTGGTCATCAAGACCTTATA
AGCAAGTGGATGACAAGCCCTATGGTGGTGGACCAGGAATGGTCATGATGTATGAGCCATTGCATGCTGCAATAAAGCAT
GCGCGCAGTGAAATGAAGGAAAACTGCAAAACAATTTACTTAAGCCCTCAAGGCAAGGTAGTTAGACAGAACGATTTAAA
ACAAATAGCAGCCCAAAAACAATCCTTGCTATTTGTTGCAGGGAGATATGAAGGAATTGATGAGCGAATCATTAGTCACC
ACGTAGATGAAGAATGGTCGCTAGGGGATTTTGTATTAAGTGGCGGTGAATTGGCCGCAATGGTATTTATTGATGCGATA
ATTCGCTTGATACCTGGAAGCCTTGGGCATTTGGGTTCAGCAGAACAAGATTCATTTATGAATGGATTACTGGACTGCCC
ACATTATACTCGGCCAGCAACTATAAATGGACTGGATGTACCTGATGTTTTATTAGGAGGAAACCATAAAGAAATAGAAC
GCTGGCGTAGGAAACAATCCCTGGGTAAAACCTGGCTCAAACGCCCGGATTTACTTGAGAAAGTACAATTAAGTGAAACA
GACAAGCAATTGCTTGCTGAGTTTAAATGCGAACACGGCGATTCCTGCTGA

Upstream 100 bases:

>100_bases
ATGTACTTGTTGTGGAAGGTGAAAAAAGACATTTAATTCCCTATCTTCCAGGACAATTTGTAATCAATGTTGATGAAAGC
CAGCAAGTAATCACTGTTGA

Downstream 100 bases:

>100_bases
GGATGAGCCAATTTGAGGAGTAGTCCATGACTAATATTATTGACCAAATTAATGCCGAACAAATGCAAGGCAAAGAAATT
CCTGATTTCAACCCAGGCGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGYEFLVALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKH
ARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAI
IRLIPGSLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSET
DKQLLAEFKCEHGDSC

Sequences:

>Translated_256_residues
MGYEFLVALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKH
ARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAI
IRLIPGSLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSET
DKQLLAEFKCEHGDSC
>Mature_255_residues
GYEFLVALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKHA
RSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAII
RLIPGSLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSETD
KQLLAEFKCEHGDSC

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=57.3770491803279, Blast_Score=288, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28731; Mature: 28600

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYEFLVALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC
YGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVA
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEE
GRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAEQDSFM
CCCCCHHHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
NGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSET
HHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKQLLAEFKCEHGDSC
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GYEFLVALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC
YGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQIAAQKQSLLFVA
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEE
GRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAEQDSFM
CCCCCHHHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
NGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKVQLSET
HHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKQLLAEFKCEHGDSC
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA