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The map label for this gene is bcr [H]

Identifier: 52840528

GI number: 52840528

Start: 320939

End: 322180

Strand: Reverse

Name: bcr [H]

Synonym: lpg0273

Alternate gene names: 52840528

Gene position: 322180-320939 (Counterclockwise)

Preceding gene: 52840532

Following gene: 52840527

Centisome position: 9.48

GC content: 40.82

Gene sequence:

>1242_bases
CTGTTAATCGTTAAAACTATTCTCATCATGTATCCAAAAACCAAACTTTCTTTCATGACTTTATTATTGTTAATTTCTTT
TGCTTCCGTGAATGCTGTCCTATTTACACCGGCATTGCCCAGCATTACTGCATTTTTCAGGATCACAGAACATGCCGCAC
AGCAGACAATTACCTGTTTTTTGGTTGGTTATGCCATAGGGCAACTTGTTTATGGCCCTACAGCGAATCGCTATGGAAGG
AAAAAAACACTTTATATAGGTATTGGCTTGCAAATTATCAGCAGTCTTCTATGTGTATTGGCCGGAATGATTTCCATGTA
CCCGTTGCTTGTTTTAGGACGATTTCTATTAGCCTTAGGATCCGGTGTAGGACTGAAGATGACTTTTACTTTGGTTAATG
AATGCTATGAGCCTCAATTAGCCGCGCAAAAAACCTCCTATCTGATGCTGGCTTTTGCGATAGCACCCGGTTTGAGTGTT
GCACTTGGCGGCATTTTAAACTCCCATTACGGCTGGGAAAGTTGCTTTTATGCAGGAACTGTTTATGGTGCTATTCTGCT
TGCCATGGTCAGGCAACTTCCCGAAACACAAAAAAATCTTCAACTCGAAGCGTTTCAAATGAAACATCTGCTCCAGGGAT
ATGTAAGTCAATTTAAAAACAAGCCATTAATCTCTGGTGGAGCATTAATGGGTGGCGCATCCTGCTTTGTCTACATTTTC
GCAGCAACGGCACCATTTATTGCCATCAATTTGCTAGGAATGAGTACAGCAGAGTATGGCGCTGCCAATATTTTACCTTC
CTTGGGTCTGACCCTCGGCTCAATCTGGTCTGCTCAATTTGCAAAAAAATACTCGCAACGCGCGGGAATCCAATTAGGCA
TTTTAATCTCAGGGTTTGCCGCACTGGCAATGATGATGGCCGCGTTAACCCATCATTCAGCGCTTATCAAGCTATTTTTT
CCCATGATATTCATCTATTTGGGACTATCTTTTATTTTTGCAAATGCTTCGACATTTGCCATGAGCCATACCTCCGATAA
AGCCCATGGCTCCGCGGTTATGAACTTCATCAATATGGGATTGGTTACAGTGGTTATTTTGGGATTAAGCTTACTTCCAA
TGTCACAATATTTATTACCAGCCATCTACATCATTCTATGCATTTTCATGATGTGGCTATTCTTTTCTTTCATAAAAAGT
AATGAAATAATTTCTTCTATCGACAGGAACAAGGAATCATGA

Upstream 100 bases:

>100_bases
ATTTACCCATGATTTCGATTTTATTATTGCAATATAGTTAATAAAAGTTAATTAATTATGATATTGTTAATTATTTTGTC
AATAATTATACTGATCTTAA

Downstream 100 bases:

>100_bases
TGAGTATACAATTGAATCATACTATCGTATGGGTAAAAGATCGGGACAAATCAGCTCACTTCCTTACAGAAATTTTGGGG
TTGCCTGAAGCAATTCCATA

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MLIVKTILIMYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITCFLVGYAIGQLVYGPTANRYGR
KKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSV
ALGGILNSHYGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIF
AATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFF
PMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKS
NEIISSIDRNKES

Sequences:

>Translated_413_residues
MLIVKTILIMYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITCFLVGYAIGQLVYGPTANRYGR
KKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSV
ALGGILNSHYGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIF
AATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFF
PMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKS
NEIISSIDRNKES
>Mature_413_residues
MLIVKTILIMYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITCFLVGYAIGQLVYGPTANRYGR
KKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSV
ALGGILNSHYGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIF
AATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFF
PMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKS
NEIISSIDRNKES

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=277, Percent_Identity=23.1046931407942, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994889, Length=351, Percent_Identity=24.5014245014245, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082312, Length=253, Percent_Identity=23.7154150197628, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790146, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44936; Mature: 44936

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVKTILIMYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITCF
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
LVGYAIGQLVYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALG
HHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
VYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIF
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHH
AATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFA
HHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
ALAMMMAALTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHH
LVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MLIVKTILIMYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITCF
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
LVGYAIGQLVYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALG
HHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
VYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIF
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHH
AATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFA
HHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
ALAMMMAALTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHH
LVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]