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The map label for this gene is pleD

Identifier: 52840485

GI number: 52840485

Start: 264958

End: 267087

Strand: Reverse

Name: pleD

Synonym: lpg0230

Alternate gene names: 52840485

Gene position: 267087-264958 (Counterclockwise)

Preceding gene: 52840486

Following gene: 52840484

Centisome position: 7.86

GC content: 38.73

Gene sequence:

>2130_bases
ATGAATAAAACTTGGCATCAAACCGCCACTGGAAACCGCTCCATTTTAAGTAATTTGCGATTAAGCAGAACTCTACTTAA
TATCATCTTTATTATTGCCCTTTCTCTGCTTTTAATCATCAATATTATTTCTTATAACCAGGTCAAAAATTTAATCTTCG
CCAGTAACTGGGTTTCTCATACTCATGAAGTCATCCAAACCATAGATACATGCCTTTATGATATAGTAGAAATTGAATCT
CGCCAGAGATTTTACTTGATTCGAGGTTCAAGCTCTCAGCTTATGGCTGATATAGATGATATGAAATCAACATTACAACT
GAATTTAGACAAACTTGAGCAATTAACCAGGGACAACCCCGAACAAAACAAACGAATCCATCGTTTTATTGATTTGGTTA
AACAAAGATTAAATTTGTTAAACCAACTTGTACAGTTAAAGGGAAGCGGCAAACTCGATACACAAGAAGGTTATGATTTA
CTTAACCAAAGTCAAGACATGTCACATCAAGTTAAAAGTCTTGGACAAGAAATCAAATCCGTTGAATTGGTGTTGCTATC
AGAAAGGAATACTGGTGTGGTTCGCTGGGCAGTTACCAGTAGTTTTATTTTAATATCCGGAGGTATTGCAAGCATCCTGT
TTTTAACGCTCGCTTTTATTCTGGCAAATATTGAATTGTCAACACGCAGAAAAACTGAACTACAGAATCAAAATACGCAA
ATACGGCTGAAGAAAATTATAGAAAGTGCCAGTGATATGATAGCAGCTTTTGGCAAAGACCAGCGATTTATTACTTTTAA
TGAAGCTTATCAGCGTGAGTTTAAACGTCTTTTTGATAAATCCATAAGCATCAATATGTCTCTGGAAGAAGCTTTCAATA
ATGCTCCTGAAAACAAAAAAAAATTAGTGCAATCCTGGAAAGAATCTCTACAAAGAGATGAAGAAACCAAAAATATCGAA
GTGAATACAGAACAGGAAAAGACTATTTATGAAATGACCTCCAAACTCATCCAAAACGGCGACAATGAAATTAAAGGTGT
GGTACACAGCGTTCGCAACATTACCAAACGGGTGCAAGAACATACTGAACTACAAGAGTCGTATGAAAAACTGGCTAATG
GAATGAAAGAACTGCAAGAGAAAAATGAACAAATTACTCTGCTGGTTGAGATGAGTGACATCATGCTGGCTTGCGGCTCC
CAGGAAGAGCTAAGTGAGGTAATGGCGAAATACTCCCAACGACTACTGCAATTTTCAAGCGGCTACCTGTTTATCATGCA
TCCTTCCAAAAATTATTTGGAAAAAGCAGCCAGTTGGGGTAATCCTCACCCGCACGATCTGACTTTTACCCCAGAGCAAT
GTTGGGCTATCCGGCTTGGTCGAATTCATTATGCAGGTTCATCTCGTATCGAATTGATGTGTAGTCATATGATGTTTGAC
GAGCAACCCGAGTTGTCTCTGCTTTGTGTACCCCTAATGGCCCAAAATGACATTTACGGCTTGTTATACCTTGAAGTTGG
CCTAAAGTTTGATGAAAACCAGCAATTACTAATAACCGCATTCGCAGAGCTTACCGCCTTGGCTTTGGCAAATGTCCGCC
TTAGAGAAAATTTACGCTATCAATCTATCCGTGATCCACTCACTGGATTATACAATCGCCGTTATCTGGAAGATTTTCTA
TTTAAACAATTACATCAGGCTGAACGAACAAAAGCCTCATTTGCCATTTTAATGCTGGATTTGGATCATTTTAAAAAGAT
CAACGATACCTTTGGTCATGACGCAGGAGATCTTGTATTAAAAGAGCTGGGGCAAATTTTAAACAATGATATTCGCCTCG
GTGATATTGCAGCCCGATATGGGGGAGAAGAATTTGTCCTGCTGCTCTATGATATTAATGCTCAAGCCGCTAAAATGAAA
GCCGAAAATTTGCGTTCGGCCATTTCCAATTTACAGGTTAAATACGGAGCACAACCTGTTGGGCAGATAACGGCCTCAAT
CGGAATATCCGTCTATCCTGACGATGCCAAATCACCTGCTGAAGTGATTGAAGCAGCTGATAAGGCTTTATACCAAGCCA
AAAACAAAGGCAGGAATAAGGTAATTTTATTCTCGGAAATATCACCCTGA

Upstream 100 bases:

>100_bases
GTTACGATAGCCATCATTTCGTTAATAACTGTTTTTTGGACTATACTCTTATCAAGGCCATGCAAAGCATCAAGCCATAA
AATTAGGATGATTCAATGCC

Downstream 100 bases:

>100_bases
TTTTGATTGCGACTCCTGACCTAAATGTTGTGGTGTTTCTTATGTCCGATAGAGTGTTTCATGTTTTATATGAGTTTTGA
GAGCAAAACAATAGATACTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIISYNQVKNLIFASNWVSHTHEVIQTIDTCLYDIVEIES
RQRFYLIRGSSSQLMADIDDMKSTLQLNLDKLEQLTRDNPEQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFNNAPENKKKLVQSWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSEVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPHPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHMMFD
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNNDIRLGDIAARYGGEEFVLLLYDINAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISP

Sequences:

>Translated_709_residues
MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIISYNQVKNLIFASNWVSHTHEVIQTIDTCLYDIVEIES
RQRFYLIRGSSSQLMADIDDMKSTLQLNLDKLEQLTRDNPEQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFNNAPENKKKLVQSWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSEVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPHPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHMMFD
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNNDIRLGDIAARYGGEEFVLLLYDINAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISP
>Mature_709_residues
MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIISYNQVKNLIFASNWVSHTHEVIQTIDTCLYDIVEIES
RQRFYLIRGSSSQLMADIDDMKSTLQLNLDKLEQLTRDNPEQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLAFILANIELSTRRKTELQNQNTQ
IRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFNNAPENKKKLVQSWKESLQRDEETKNIE
VNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGS
QEELSEVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPHPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHMMFD
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFL
FKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNNDIRLGDIAARYGGEEFVLLLYDINAQAAKMK
AENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=42.8571428571429, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=36.6279069767442, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=34.9112426035503, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1786584, Length=249, Percent_Identity=28.9156626506024, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=36.4705882352941, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=34.7058823529412, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787816, Length=199, Percent_Identity=32.6633165829146, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=35.7575757575758, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=30.635838150289, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081974, Length=179, Percent_Identity=33.5195530726257, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI87081977, Length=224, Percent_Identity=32.1428571428571, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=31.0975609756098, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 81041; Mature: 81041

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIISYNQVKNLIFASNWVSH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THEVIQTIDTCLYDIVEIESRQRFYLIRGSSSQLMADIDDMKSTLQLNLDKLEQLTRDNP
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCC
EQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDLLNQSQDMSHQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
VELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLAFILANIELSTRRKTELQNQNTQ
EEEEEEECCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
IRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFNNAPENKK
HHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHH
KLVQSWKESLQRDEETKNIEVNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQE
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGSQEELSEVMAKYSQRLLQFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKAASWGNPHPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHMMFD
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHEECEEEECCCCHHHHHHHHHHCC
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITAFAELTALALANVRLRENLRY
CCCCCEEEEEEHHCCCCCEEEEEEEECCEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCH
QSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCCCCHHHHHH
KELGQILNNDIRLGDIAARYGGEEFVLLLYDINAQAAKMKAENLRSAISNLQVKYGAQPV
HHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHCCCCHH
GQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISP
HHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIISYNQVKNLIFASNWVSH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THEVIQTIDTCLYDIVEIESRQRFYLIRGSSSQLMADIDDMKSTLQLNLDKLEQLTRDNP
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCC
EQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDLLNQSQDMSHQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
VELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLAFILANIELSTRRKTELQNQNTQ
EEEEEEECCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
IRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKRLFDKSISINMSLEEAFNNAPENKK
HHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHH
KLVQSWKESLQRDEETKNIEVNTEQEKTIYEMTSKLIQNGDNEIKGVVHSVRNITKRVQE
HHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HTELQESYEKLANGMKELQEKNEQITLLVEMSDIMLACGSQEELSEVMAKYSQRLLQFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKAASWGNPHPHDLTFTPEQCWAIRLGRIHYAGSSRIELMCSHMMFD
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHEECEEEECCCCHHHHHHHHHHCC
EQPELSLLCVPLMAQNDIYGLLYLEVGLKFDENQQLLITAFAELTALALANVRLRENLRY
CCCCCEEEEEEHHCCCCCEEEEEEEECCEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCH
QSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHDAGDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCCCCHHHHHH
KELGQILNNDIRLGDIAARYGGEEFVLLLYDINAQAAKMKAENLRSAISNLQVKYGAQPV
HHHHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHCCCCHH
GQITASIGISVYPDDAKSPAEVIEAADKALYQAKNKGRNKVILFSEISP
HHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]