Click here to switch to the map view.

The map label for this gene is artJ [H]

Identifier: 52840343

GI number: 52840343

Start: 98310

End: 99086

Strand: Reverse

Name: artJ [H]

Synonym: lpg0088

Alternate gene names: 52840343

Gene position: 99086-98310 (Counterclockwise)

Preceding gene: 52840344

Following gene: 52840342

Centisome position: 2.92

GC content: 36.55

Gene sequence:

>777_bases
TTGCTCCTCCTAAATTTTAAGTACATCCTAAATGGACTTAAAAACATGAAACGATTCATGTTCATTCTTTTGATGTTTTT
AAATTTTTACACCTTTGGAAAACCACTTATCATTGGTGTTCCGGAATTTGCGCCCCCATTTGTATCAAGATCTGGAGGAG
GAGGAGAGACATATTTTGGATTTAACATCGATTTGATGAATTCCATTTGCAAGATCATTGAAATGGAATGCCAATACAAG
GGCATGAATATTCAACAGCTATATACCAATCTTAACCAAGGGAAGCTTGATATTATGCTGGCTCCTACTCCAATCGTATC
CACTGTTGGCACTAATTATATATTCAGTCTTCCCTATTTACCGAGCAATGCACAGGTGATAACCCTGAAAGCTAACAATG
ACATCAATAGCTTAGCAGATCTGAAAGGTAAAAAGGTTGGCACTTTAAAATACACGTTGTATGGTAGCCTCCTTGCAGAA
CATTTTCAGGATTATTTTGACCTGGTACAATTTTCCAAACTGTCTGACATGGCCCTCGCATTATCAAACCAAAAAATTGA
AGCCATTGTTCTTAACACAAATGCAGCGAAATATGCGATGAGCAATTCTGCAACTGAGTTAAAATTTGTGGGCGATAAAA
TGGCTATAGGTGATGGTTATGGAATACTGGCCCTCAAAAATAATGCTCCTCTCATTATGAAAATCAATAAGGCTTTATTA
CAAATAGAAAAAGACGGGACCTATTTGGAAATCTATAATACCTATTTCGGTGATTAG

Upstream 100 bases:

>100_bases
AGAAAAAACCAATTTTGCCAATGCTCTGGATCGTTGGCTTGCAAAAAGCAAATAAAAGCATCAATTAAAAAACATATTGA
CTTTAATTTGACAAAATCGA

Downstream 100 bases:

>100_bases
GATATTATCCTGAGCAATTCACCAGGCATTTCAAAAACCCGAAACTAGATCCAACTCCAGAATTCAGGTTAAACTAATAC
CAATTGAACCTGCCTCTCAT

Product: glutamine ABC transporter

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLLLNFKYILNGLKNMKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYFGFNIDLMNSICKIIEMECQYK
GMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSLPYLPSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAE
HFQDYFDLVQFSKLSDMALALSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIMKINKALL
QIEKDGTYLEIYNTYFGD

Sequences:

>Translated_258_residues
MLLLNFKYILNGLKNMKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYFGFNIDLMNSICKIIEMECQYK
GMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSLPYLPSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAE
HFQDYFDLVQFSKLSDMALALSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIMKINKALL
QIEKDGTYLEIYNTYFGD
>Mature_258_residues
MLLLNFKYILNGLKNMKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYFGFNIDLMNSICKIIEMECQYK
GMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSLPYLPSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAE
HFQDYFDLVQFSKLSDMALALSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIMKINKALL
QIEKDGTYLEIYNTYFGD

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=27.8688524590164, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=25.0980392156863, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787088, Length=216, Percent_Identity=27.3148148148148, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787031, Length=237, Percent_Identity=28.2700421940928, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=26.0089686098655, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788648, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28846; Mature: 28846

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLNFKYILNGLKNMKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYFG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEE
FNIDLMNSICKIIEMECQYKGMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSLPYL
EEHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCCHHHHCCCCEEEECCCC
PSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAEHFQDYFDLVQFSKLSDMALA
CCCCEEEEEECCCCHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIMKINKALL
ECCCCEEEEEEECCCHHEEECCCCCEEEEECCEEEECCCEEEEEECCCCCEEEEECCEEE
QIEKDGTYLEIYNTYFGD
EEECCCCEEEEECCCCCC
>Mature Secondary Structure
MLLLNFKYILNGLKNMKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYFG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEE
FNIDLMNSICKIIEMECQYKGMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSLPYL
EEHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCCHHHHCCCCEEEECCCC
PSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAEHFQDYFDLVQFSKLSDMALA
CCCCEEEEEECCCCHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIMKINKALL
ECCCCEEEEEEECCCHHEEECCCCCEEEEECCEEEECCCEEEEEECCCCCEEEEECCEEE
QIEKDGTYLEIYNTYFGD
EEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]