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The map label for this gene is artJ

Identifier: 52840293

GI number: 52840293

Start: 43827

End: 44570

Strand: Reverse

Name: artJ

Synonym: lpg0037

Alternate gene names: 52840293

Gene position: 44570-43827 (Counterclockwise)

Preceding gene: 52840294

Following gene: 52840292

Centisome position: 1.31

GC content: 35.48

Gene sequence:

>744_bases
ATGGTTCGCCTCAGAATTTATTTATGTTCTTTGCTACTGGTTATTCCCTTTTATAGAGCCTATGCTCTTAATTTGACCAT
AGGGACCTTATCTTATAATCCTCCGTTTGAAACTCAAGTCAACGATTCTTCCAAGAAAAATGAGTTTTATGGTTTTGAAA
TTGAACTCATGGATGAGATTTGCGAGCGCATCAATGCAAAATGCCAATACAAGCCATTGTTATTTGAACAAATTTTAGCT
GAAATTAATTCTGGTGCAATCAATTTAGGCATTGGTAGCATTAATATTACGCCTGAAAGAAGCGAACGATTTTTATTCAG
CCTTCCCTACAAAGCCAGCTATCACCAGTTTCTTATACCATCTAACTCGAATATAAAAAAAATTGACGATCTCAAAGGAA
AGACTATAGGAATTCATTTAGGTTCACCTACTGCCGATTATTTAAAAGCCTACTTTAACAATAATATTCAATTTAAATAT
TACGAAACATTTATGGATTTACTTAATGGGCTGACAGATCCGCAAGTTAGCGCAATAGTGACTAATAGCGATCAAGCGCA
ATACTGGGCATCGAGTACACCGGACTGCAAGTTATTGGGCGACAGTTTTCCTTTTGGAGTAGGATTTGGAGTTGTCGCGA
GATTAGATCAAGCAGAACTGATTGAAAAAATCAATCAGGCATTAATTGAGATTGAAAATGATGGGACTTACCTTAAAATA
TATAACCTCTTCTTTCAACCTTAA

Upstream 100 bases:

>100_bases
TAAAGCGCCCTGATTTATGAAAAAATATAAATCTAACCGTTCTTTTATTTTTCAAAAATAAGGCTAAAATAATAAATAAC
TATATAAAAAGGATATTTAA

Downstream 100 bases:

>100_bases
GTATTATTTCGACCGACAAAGGACTCCAGTGCTTAATAAATCTATTGATTTTTGTCTATAATCTGAGAAACAATAAAAGG
TTAACATCATTCTATCAATA

Product: arginine 3rd transport system periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSSKKNEFYGFEIELMDEICERINAKCQYKPLLFEQILA
EINSGAINLGIGSINITPERSERFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKY
YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQAELIEKINQALIEIENDGTYLKI
YNLFFQP

Sequences:

>Translated_247_residues
MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSSKKNEFYGFEIELMDEICERINAKCQYKPLLFEQILA
EINSGAINLGIGSINITPERSERFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKY
YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQAELIEKINQALIEIENDGTYLKI
YNLFFQP
>Mature_247_residues
MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSSKKNEFYGFEIELMDEICERINAKCQYKPLLFEQILA
EINSGAINLGIGSINITPERSERFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKY
YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQAELIEKINQALIEIENDGTYLKI
YNLFFQP

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=28.2258064516129, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=26.6932270916335, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=28.7937743190661, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=27.0742358078603, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=26.5060240963855, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28098; Mature: 28098

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSSKKNEFYGFEIELMDEI
CEEEHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
CERINAKCQYKPLLFEQILAEINSGAINLGIGSINITPERSERFLFSLPYKASYHQFLIP
HHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEC
SNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKYYETFMDLLNGLTDPQVSAIV
CCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCEEEEE
TNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQAELIEKINQALIEIENDGTYLKI
ECCCCHHEECCCCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEE
YNLFFQP
EEEEECC
>Mature Secondary Structure
MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSSKKNEFYGFEIELMDEI
CEEEHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
CERINAKCQYKPLLFEQILAEINSGAINLGIGSINITPERSERFLFSLPYKASYHQFLIP
HHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEC
SNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKYYETFMDLLNGLTDPQVSAIV
CCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCEEEEE
TNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQAELIEKINQALIEIENDGTYLKI
ECCCCHHEECCCCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEE
YNLFFQP
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]