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The map label for this gene is pit

Identifier: 52840283

GI number: 52840283

Start: 33175

End: 34170

Strand: Reverse

Name: pit

Synonym: lpg0027

Alternate gene names: 52840283

Gene position: 34170-33175 (Counterclockwise)

Preceding gene: 52840284

Following gene: 52840281

Centisome position: 1.01

GC content: 42.77

Gene sequence:

>996_bases
ATGGACTACAATACGATATTCATTCTATTCGTCATATTTATTGCGTTCGTATTTGATTTTATCAACGGATTTCATGATGC
CGCCAATTCGATTGCAACCATAGTCACTACAGGTGTTTTGACGCCAAGACAAGCTGTACTATGGGCTGCTTTTTTTAACT
TCATTGCTTTTCTGATTTTCAATTTAACCGTAGCCCAAACAATAGGCTACGGACTAATCGATACGGATCTGGTGGATACT
TATTTTATTTTAGCAGCCCTGATGGGAGCGATATTCTGGAATTTGGTGACTTGGTATTATGGACTGCCATCAAGCTCCTC
TCATGCCTTAATTGGCGGATTAGCTGGTGCGGCAATAGCCAAGGGAGGCCTGTCTTCACTCAAGATTACAGGTTTTGCCA
AAGTAGCGGCAGGGATATTTATATCTCCCGTTCTGGGATTATTGATTGGCTTATTATTTACTCATATTTTCTCTTTGTTT
CTCAGACATAAGAACGAAGAGGAACTGAATACATTATTTAAAAGTTTTCAACTGATATCTTCAGCATTATTGAGTATCAC
GCATGGAGGAAATGACGCACAGAAAACGATGGGTATCATCGCGGTATTACTTTTTTCCGCATCTTGGCTTGATGGTGTAT
TTTATGTGCCATTTTGGGTCGTCATTTCATGTCATGGGGTAATTAGTCTGGGGACTTTGGCTGGTGGCTGGAGGATTGTG
CATACCATGGGAACTAAAATTACCAAACTTAACACCCTGAAGGGTTGTGCTGCTGAAACAGGAGCAGCCATTACCATTTT
CGCTGCAACAGAATTTGGAGTACCTGTCTCAACCACCCATACGGTCACTGGCTCCATTGCGGGAGTTGGGCTAATCAGTG
GCATAGGTGGCGCCCACTGGAAAGTTTTACGCAGGATATTCTACTCCTGGTTGTTGACCATTCCAGCAGCAGCCTTAGTT
GCTGCTGCTATTGTAAACATCGCACCAACAGCTTAG

Upstream 100 bases:

>100_bases
GGAGCAGTTGAACTTCCCTATATTATTAAGCAATTGATGAATGCTGTATCAAAATTAATGACTCAGAGCAGTTATTATAT
TTAACAGGATATGATTACAT

Downstream 100 bases:

>100_bases
AGTCCAATCTCACTCGGCGGCACAATCCCCCTCAACACTCAAAGCACTATTTCCCCTGCTAAATGCAAAATAGACAGCCA
TACCTACCGCCATCCAAATT

Product: low affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQAVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDT
YFILAALMGAIFWNLVTWYYGLPSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPFWVVISCHGVISLGTLAGGWRIV
HTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPVSTTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALV
AAAIVNIAPTA

Sequences:

>Translated_331_residues
MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQAVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDT
YFILAALMGAIFWNLVTWYYGLPSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPFWVVISCHGVISLGTLAGGWRIV
HTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPVSTTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALV
AAAIVNIAPTA
>Mature_331_residues
MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQAVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDT
YFILAALMGAIFWNLVTWYYGLPSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPFWVVISCHGVISLGTLAGGWRIV
HTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPVSTTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALV
AAAIVNIAPTA

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=167, Percent_Identity=28.7425149700599, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5803173, Length=156, Percent_Identity=31.4102564102564, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1789360, Length=222, Percent_Identity=31.981981981982, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.7972350230415, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25146401, Length=167, Percent_Identity=29.3413173652695, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=175, Percent_Identity=30.8571428571429, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=161, Percent_Identity=29.1925465838509, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=26.6272189349112, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32566716, Length=178, Percent_Identity=29.7752808988764, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=170, Percent_Identity=27.0588235294118, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356511, Length=185, Percent_Identity=29.1891891891892, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35203; Mature: 35203

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQAVLWAAFFNFIAFLIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGLPSSSSHALIGGLAGAAIA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLFLRHKNEEELNTLFKSFQLIS
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPFWVVISCHGVISLGTLAGGWRIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
HTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPVSTTHTVTGSIAGVGLISGIGGAHW
EHHCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCHHHH
KVLRRIFYSWLLTIPAAALVAAAIVNIAPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQAVLWAAFFNFIAFLIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGLPSSSSHALIGGLAGAAIA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLFLRHKNEEELNTLFKSFQLIS
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPFWVVISCHGVISLGTLAGGWRIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
HTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPVSTTHTVTGSIAGVGLISGIGGAHW
EHHCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEECHHHHHHHHHHCCCHHHH
KVLRRIFYSWLLTIPAAALVAAAIVNIAPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]