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The map label for this gene is ywaA [H]

Identifier: 52787761

GI number: 52787761

Start: 3908070

End: 3909161

Strand: Direct

Name: ywaA [H]

Synonym: BLi04084

Alternate gene names: 52787761

Gene position: 3908070-3909161 (Clockwise)

Preceding gene: 52787760

Following gene: 52787762

Centisome position: 92.55

GC content: 47.62

Gene sequence:

>1092_bases
ATGACAAAACAAACGATCAGCGTACAGCTCAGTACAGCAAAAAAGCAAAAGCCAGAGGCTGACAAGCTCGAATTCGGCCG
GACCTTTACCGACCACATGTTTATCATGGACTATACGGCTGAAAACGGCTGGCACGATCCGAGAATCGTTCCTTACCAGC
CGATTGAAATGGACCCGGCTGCAATGGTTTACCACTACGGACAATCCGTTTTTGAAGGATTAAAAGCTTATTTATCAAGC
GAAGGCAGAGTTCTTCTGTTCAGACCTGAAAAAAACTTCGAGAGACTCAATAAATCCAACGACCGCCTCTGCATTCCCCG
GGTTGATCCTGAAATCGTTCTGGAAGGGCTGAAGCAGCTGGTTCAGATCGACAAGGAATGGATTCCTCAAGCTGAGGGGA
CATCCCTTTATATCCGTCCGTTCATTATTTCAACAGAACCGTACCTTGGCGTCGCCCCATCCAATATGTATAAAATGCTG
ATCATTTTATCGCCGGTCGGATCTTATTACAAAGAAGGCATCCGCCCTGTGAAAATTGCTGTTGAAAGCGAATTTGTCCG
TGCTGTGGCAGGCGGTACAGGCAATGCAAAAACGGCCGGAAACTACGCTGCGAGCCTGAAGGCTCAGGAAGTTGCGGAAA
GCAAAGGCTTCTCACAAGTGCTGTGGCTTGACGGAGTTGAAAAGAAATACATTGAAGAAGTAGGCAGCATGAACATCTTC
TTCAAAATCAGCGGTGAAATTGTCACTCCGGCTCTAAACGGAAGCATTTTGGAAGGCATTACGAGAAACTCCGTCATCCA
TCTCTTAAAACAATGGGGACTCTCCGTAACCGAAAGAAAAATTTCAGTTGATGAACTGGTTCAGGCTCACAAAGACGGCC
TGCTTGAGGAAGCATTCGGCACCGGAACCGCCGCTGTCATTTCCCCCGTCGGCGAGCTGATCTGGAAAGACGAAAGCCTT
GTGATCAACAACGGTCAAACTGGAGAAATCGCCAAAAGGCTCTACCAAACGATCACCGGTATTCAAAAAGGCGCTTTGCC
TGACACATTCGGCTGGACAGTTGAAGTTGATAAAGTAAGCCAGTCCTGCTAA

Upstream 100 bases:

>100_bases
TACAAATTTCTCTGAAATCTATTGCGATTATCAAGAGAAGGTTTAAAATAGAAGATAAATATTTTTTGGAAAAAGACTAG
AAACGGCAGGGAGAATGAAT

Downstream 100 bases:

>100_bases
GCGGTTTAAGCTCTCTTTTGCAAAAAAGAGGGCTTGCACCCTTATCGTAAAATTCGCCGGCGCCAGGCTCTGTTTCCACC
CTACTTGCCTCTTTTTCAAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKQTISVQLSTAKKQKPEADKLEFGRTFTDHMFIMDYTAENGWHDPRIVPYQPIEMDPAAMVYHYGQSVFEGLKAYLSS
EGRVLLFRPEKNFERLNKSNDRLCIPRVDPEIVLEGLKQLVQIDKEWIPQAEGTSLYIRPFIISTEPYLGVAPSNMYKML
IILSPVGSYYKEGIRPVKIAVESEFVRAVAGGTGNAKTAGNYAASLKAQEVAESKGFSQVLWLDGVEKKYIEEVGSMNIF
FKISGEIVTPALNGSILEGITRNSVIHLLKQWGLSVTERKISVDELVQAHKDGLLEEAFGTGTAAVISPVGELIWKDESL
VINNGQTGEIAKRLYQTITGIQKGALPDTFGWTVEVDKVSQSC

Sequences:

>Translated_363_residues
MTKQTISVQLSTAKKQKPEADKLEFGRTFTDHMFIMDYTAENGWHDPRIVPYQPIEMDPAAMVYHYGQSVFEGLKAYLSS
EGRVLLFRPEKNFERLNKSNDRLCIPRVDPEIVLEGLKQLVQIDKEWIPQAEGTSLYIRPFIISTEPYLGVAPSNMYKML
IILSPVGSYYKEGIRPVKIAVESEFVRAVAGGTGNAKTAGNYAASLKAQEVAESKGFSQVLWLDGVEKKYIEEVGSMNIF
FKISGEIVTPALNGSILEGITRNSVIHLLKQWGLSVTERKISVDELVQAHKDGLLEEAFGTGTAAVISPVGELIWKDESL
VINNGQTGEIAKRLYQTITGIQKGALPDTFGWTVEVDKVSQSC
>Mature_362_residues
TKQTISVQLSTAKKQKPEADKLEFGRTFTDHMFIMDYTAENGWHDPRIVPYQPIEMDPAAMVYHYGQSVFEGLKAYLSSE
GRVLLFRPEKNFERLNKSNDRLCIPRVDPEIVLEGLKQLVQIDKEWIPQAEGTSLYIRPFIISTEPYLGVAPSNMYKMLI
ILSPVGSYYKEGIRPVKIAVESEFVRAVAGGTGNAKTAGNYAASLKAQEVAESKGFSQVLWLDGVEKKYIEEVGSMNIFF
KISGEIVTPALNGSILEGITRNSVIHLLKQWGLSVTERKISVDELVQAHKDGLLEEAFGTGTAAVISPVGELIWKDESLV
INNGQTGEIAKRLYQTITGIQKGALPDTFGWTVEVDKVSQSC

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=40.5797101449275, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=40.5797101449275, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=40.5797101449275, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI50658084, Length=348, Percent_Identity=39.0804597701149, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI296010902, Length=352, Percent_Identity=36.6477272727273, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=37.1014492753623, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI258614015, Length=283, Percent_Identity=40.2826855123675, Blast_Score=219, Evalue=2e-57,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=30.7453416149068, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=370, Percent_Identity=38.3783783783784, Blast_Score=260, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17565728, Length=359, Percent_Identity=35.3760445682451, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6322608, Length=347, Percent_Identity=42.0749279538905, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=41.4492753623188, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24641779, Length=364, Percent_Identity=39.8351648351648, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40327; Mature: 40196

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTISVQLSTAKKQKPEADKLEFGRTFTDHMFIMDYTAENGWHDPRIVPYQPIEMDPA
CCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHH
AMVYHYGQSVFEGLKAYLSSEGRVLLFRPEKNFERLNKSNDRLCIPRVDPEIVLEGLKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
VQIDKEWIPQAEGTSLYIRPFIISTEPYLGVAPSNMYKMLIILSPVGSYYKEGIRPVKIA
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCHHEEEEEEECCHHHHHHCCCCEEEEE
VESEFVRAVAGGTGNAKTAGNYAASLKAQEVAESKGFSQVLWLDGVEKKYIEEVGSMNIF
EHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCEEEE
FKISGEIVTPALNGSILEGITRNSVIHLLKQWGLSVTERKISVDELVQAHKDGLLEEAFG
EEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHC
TGTAAVISPVGELIWKDESLVINNGQTGEIAKRLYQTITGIQKGALPDTFGWTVEVDKVS
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
QSC
CCC
>Mature Secondary Structure 
TKQTISVQLSTAKKQKPEADKLEFGRTFTDHMFIMDYTAENGWHDPRIVPYQPIEMDPA
CCCEEEEEEECCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHH
AMVYHYGQSVFEGLKAYLSSEGRVLLFRPEKNFERLNKSNDRLCIPRVDPEIVLEGLKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
VQIDKEWIPQAEGTSLYIRPFIISTEPYLGVAPSNMYKMLIILSPVGSYYKEGIRPVKIA
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCHHEEEEEEECCHHHHHHCCCCEEEEE
VESEFVRAVAGGTGNAKTAGNYAASLKAQEVAESKGFSQVLWLDGVEKKYIEEVGSMNIF
EHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCEEEE
FKISGEIVTPALNGSILEGITRNSVIHLLKQWGLSVTERKISVDELVQAHKDGLLEEAFG
EEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHC
TGTAAVISPVGELIWKDESLVINNGQTGEIAKRLYQTITGIQKGALPDTFGWTVEVDKVS
CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
QSC
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]