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The map label for this gene is ywhE [H]

Identifier: 52787655

GI number: 52787655

Start: 3803607

End: 3805682

Strand: Direct

Name: ywhE [H]

Synonym: BLi03977

Alternate gene names: 52787655

Gene position: 3803607-3805682 (Clockwise)

Preceding gene: 52787654

Following gene: 52787658

Centisome position: 90.08

GC content: 50.63

Gene sequence:

>2076_bases
ATGGACATAATGACGGACAAGCGGGTCCGTTTAACCATCAAGACGATACGCGCATTTTTGTTCATGGGAGCATTGATGCT
GATCTTCCTTTCAATCGTTGTCATGACGGTGGTCTTGGCGGCCAAATGGCAAGGCGCTCCTTCGATTCAGGTGCCGAGGT
CTACCGTGCTGTATGACGGAAGCGGAAAGCAAATGGGGGAAACGCATTACGGGCAAAAGCGGTACTGGGTTTCGCTGAAG
AACATCAATCCCGCAGTCATCGATGCCACGCTTGCCGTTGAAGACCGGAACTTTTTCCGGCACCACGGCTTTGACTATAT
GAGGATGGGCGGCGCCCTCATTGCCGATTTAAAGGCGATGTCCAAAGTTCAGGGAGCCAGCACCATCACCCAGCAATATG
CGAGAAACCTCTACCTCGGCCACGATAAAACATGGAAAAGAAAGTGGAATGAAGCTTTTTATACGATAAGGCTTGAACAA
AACTACACAAAGAAAGATATTTTGGAAGGCTATTTAAATACGATTTATTACGGGCATGGAGCATACGGAATCGAAGCGGC
TTCCCGGCTGTATTTTGGCAAGCGGGCCAAAGATATCAATACGGCGGAGGCTGCAATGCTTGCCGGCATTCCAAAGGGTC
CTTCAGTTTATTCGCCCTTTGTCAATGAAAAAAAAGCGAAAGAACGCCAGGAAATGATCCTGACAATGATGGAGAAACAA
AACAAGCTGACGGAAATGCAGGCGGCAGCACTGAAAAAAATGCCGCTCCGCTTTGAAAAACAGGATAAGCAAACAACCGA
AAAAACCGCTCCGTACTTTTATGACGAAGCTGTTAAAGAAATTTCGCGATTGCTGCGTTTGACGCCGGAGCAAGCGGAAA
CAGGCGGCTACAACGTATTCACCACGCTCAACCCGCGTCTCCAAAAAATTGCCGAGGATACGATCGAACACACGATAGAT
TCGTCATCCGACATTCAGGCGGGATTTGCAGCGATCAACCCGTCCGACGGCAGCGTTCTCGCGCTTGTCGGAGGACGCGA
CTACGAAAAAAGCCCTTTTAACCGCGTAACGCAGGCTATGCGGCAGCCCGGCTCCACCATGAAGCCTTTCCTTTATTATT
CGGCGGTTCAAAACGGCTTTACACCGGCCACCCGAATGAGGAGCGCGGAAACGACATTTGAGCTCGGGCAAGGCAGCGCT
TATTCCCCGAGCAACTATCACGGCTATTATGCAGACGGTCCGATCACGCTTCTGCAGGCACTGGCTTTGTCCGATAACAT
TTACGCCGTGAAAACCCACCTTTTCCTCGGCATGGACAAACTGATTGATGCCGCAAAGCAATTCGGCATCAACAGCCCGC
TGCAAAAAGTGCCGTCGCTTGCGCTCGGAACATCCCCTGTGAAGCCGATTGAAATGGTGAACGGATACGCCATGTTTGCC
AACGGCGGGAAAAAAATCGAACCTTCCTTTATTACGAAAATTACCGATCCGAACGGGAAGGTTCTTTATGAAAAACCGAA
GCGGAGCAAACAAATCCTTGACCCAAAAGCGGCTTTTATCACGGCGAACATGATGAGCGGGATGTTCGACAAATCACTCA
ACGGCTATACATCTGTTACCGGCCGGACGATCGCAGATCAGCTGACGCGCCGATACGCCGGAAAATCGGGAACGACCAGC
ACCGACAGCTGGATGATCGGCTTTTATCCCGGTCTTGCTTCAGGCGTGTGGACGGGATATGACAAAGAGCGGACAATCGA
TGCCGTCGCAGAGAAAAACTATGCCAAACAAATTTGGGCTGAGTTTATGGAAAAAGCGCTTGAAGACGCGCCCGCCGCAG
CTTTAATGCCGCCTGAAGGCGTGAAAGGGATGTACATCGACCCGGCTACAGGTTATGCGGCGGCGCCCAGCTGCCCGTCC
AAATATTTCGCTTATTTTATCAAAGGTACTGAACCCGAGCAGGTTTGCTATGGAAAGGAAATCTATAAGGAGCAAAACGC
CGGGCACGAAAAGCCCGCCGCTCCCCCCCATAAGCGGCAAAAGTGGTGGGAAAAATGGTTTAAAAAAGGAGAATAA

Upstream 100 bases:

>100_bases
CGCAGAGGGTCTGCCGTTTTTTCCTTCCCGCTTGTTTAAATCCTCCTCTTTTTCCTCATAATGATAAGAACATTTTATTT
ATCCGGGAGGAGATTTGAAC

Downstream 100 bases:

>100_bases
ACTTTTTAGGGGGATAGACGAACCCCCCGGACTTCATGGCAGTCCGGGGGGTTTTTCCTATTGCTGGAGCAGTCCCTTTT
TCAGCTCGTCTGATGAACGG

Product: YwhE

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MDIMTDKRVRLTIKTIRAFLFMGALMLIFLSIVVMTVVLAAKWQGAPSIQVPRSTVLYDGSGKQMGETHYGQKRYWVSLK
NINPAVIDATLAVEDRNFFRHHGFDYMRMGGALIADLKAMSKVQGASTITQQYARNLYLGHDKTWKRKWNEAFYTIRLEQ
NYTKKDILEGYLNTIYYGHGAYGIEAASRLYFGKRAKDINTAEAAMLAGIPKGPSVYSPFVNEKKAKERQEMILTMMEKQ
NKLTEMQAAALKKMPLRFEKQDKQTTEKTAPYFYDEAVKEISRLLRLTPEQAETGGYNVFTTLNPRLQKIAEDTIEHTID
SSSDIQAGFAAINPSDGSVLALVGGRDYEKSPFNRVTQAMRQPGSTMKPFLYYSAVQNGFTPATRMRSAETTFELGQGSA
YSPSNYHGYYADGPITLLQALALSDNIYAVKTHLFLGMDKLIDAAKQFGINSPLQKVPSLALGTSPVKPIEMVNGYAMFA
NGGKKIEPSFITKITDPNGKVLYEKPKRSKQILDPKAAFITANMMSGMFDKSLNGYTSVTGRTIADQLTRRYAGKSGTTS
TDSWMIGFYPGLASGVWTGYDKERTIDAVAEKNYAKQIWAEFMEKALEDAPAAALMPPEGVKGMYIDPATGYAAAPSCPS
KYFAYFIKGTEPEQVCYGKEIYKEQNAGHEKPAAPPHKRQKWWEKWFKKGE

Sequences:

>Translated_691_residues
MDIMTDKRVRLTIKTIRAFLFMGALMLIFLSIVVMTVVLAAKWQGAPSIQVPRSTVLYDGSGKQMGETHYGQKRYWVSLK
NINPAVIDATLAVEDRNFFRHHGFDYMRMGGALIADLKAMSKVQGASTITQQYARNLYLGHDKTWKRKWNEAFYTIRLEQ
NYTKKDILEGYLNTIYYGHGAYGIEAASRLYFGKRAKDINTAEAAMLAGIPKGPSVYSPFVNEKKAKERQEMILTMMEKQ
NKLTEMQAAALKKMPLRFEKQDKQTTEKTAPYFYDEAVKEISRLLRLTPEQAETGGYNVFTTLNPRLQKIAEDTIEHTID
SSSDIQAGFAAINPSDGSVLALVGGRDYEKSPFNRVTQAMRQPGSTMKPFLYYSAVQNGFTPATRMRSAETTFELGQGSA
YSPSNYHGYYADGPITLLQALALSDNIYAVKTHLFLGMDKLIDAAKQFGINSPLQKVPSLALGTSPVKPIEMVNGYAMFA
NGGKKIEPSFITKITDPNGKVLYEKPKRSKQILDPKAAFITANMMSGMFDKSLNGYTSVTGRTIADQLTRRYAGKSGTTS
TDSWMIGFYPGLASGVWTGYDKERTIDAVAEKNYAKQIWAEFMEKALEDAPAAALMPPEGVKGMYIDPATGYAAAPSCPS
KYFAYFIKGTEPEQVCYGKEIYKEQNAGHEKPAAPPHKRQKWWEKWFKKGE
>Mature_691_residues
MDIMTDKRVRLTIKTIRAFLFMGALMLIFLSIVVMTVVLAAKWQGAPSIQVPRSTVLYDGSGKQMGETHYGQKRYWVSLK
NINPAVIDATLAVEDRNFFRHHGFDYMRMGGALIADLKAMSKVQGASTITQQYARNLYLGHDKTWKRKWNEAFYTIRLEQ
NYTKKDILEGYLNTIYYGHGAYGIEAASRLYFGKRAKDINTAEAAMLAGIPKGPSVYSPFVNEKKAKERQEMILTMMEKQ
NKLTEMQAAALKKMPLRFEKQDKQTTEKTAPYFYDEAVKEISRLLRLTPEQAETGGYNVFTTLNPRLQKIAEDTIEHTID
SSSDIQAGFAAINPSDGSVLALVGGRDYEKSPFNRVTQAMRQPGSTMKPFLYYSAVQNGFTPATRMRSAETTFELGQGSA
YSPSNYHGYYADGPITLLQALALSDNIYAVKTHLFLGMDKLIDAAKQFGINSPLQKVPSLALGTSPVKPIEMVNGYAMFA
NGGKKIEPSFITKITDPNGKVLYEKPKRSKQILDPKAAFITANMMSGMFDKSLNGYTSVTGRTIADQLTRRYAGKSGTTS
TDSWMIGFYPGLASGVWTGYDKERTIDAVAEKNYAKQIWAEFMEKALEDAPAAALMPPEGVKGMYIDPATGYAAAPSCPS
KYFAYFIKGTEPEQVCYGKEIYKEQNAGHEKPAAPPHKRQKWWEKWFKKGE

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=616, Percent_Identity=27.1103896103896, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87082258, Length=272, Percent_Identity=33.8235294117647, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788867, Length=543, Percent_Identity=27.8084714548803, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789601, Length=215, Percent_Identity=35.3488372093023, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77292; Mature: 77292

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIMTDKRVRLTIKTIRAFLFMGALMLIFLSIVVMTVVLAAKWQGAPSIQVPRSTVLYDG
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
SGKQMGETHYGQKRYWVSLKNINPAVIDATLAVEDRNFFRHHGFDYMRMGGALIADLKAM
CCCCCCCCCCCCEEEEEEECCCCHHHEEEEEEECCCHHHHHCCCHHHHHCCHHHHHHHHH
SKVQGASTITQQYARNLYLGHDKTWKRKWNEAFYTIRLEQNYTKKDILEGYLNTIYYGHG
HHHCCHHHHHHHHHHHEECCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEECCC
AYGIEAASRLYFGKRAKDINTAEAAMLAGIPKGPSVYSPFVNEKKAKERQEMILTMMEKQ
CHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NKLTEMQAAALKKMPLRFEKQDKQTTEKTAPYFYDEAVKEISRLLRLTPEQAETGGYNVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEE
TTLNPRLQKIAEDTIEHTIDSSSDIQAGFAAINPSDGSVLALVGGRDYEKSPFNRVTQAM
EECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHH
RQPGSTMKPFLYYSAVQNGFTPATRMRSAETTFELGQGSAYSPSNYHGYYADGPITLLQA
HCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCHHCCCCCCCCCCCCCCCEECCCHHHHHHH
LALSDNIYAVKTHLFLGMDKLIDAAKQFGINSPLQKVPSLALGTSPVKPIEMVNGYAMFA
HHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHCCCCCCCHHHHHCCEEEEE
NGGKKIEPSFITKITDPNGKVLYEKPKRSKQILDPKAAFITANMMSGMFDKSLNGYTSVT
CCCCEECCHHHEEEECCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GRTIADQLTRRYAGKSGTTSTDSWMIGFYPGLASGVWTGYDKERTIDAVAEKNYAKQIWA
CHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EFMEKALEDAPAAALMPPEGVKGMYIDPATGYAAAPSCPSKYFAYFIKGTEPEQVCYGKE
HHHHHHHHCCCCEECCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
IYKEQNAGHEKPAAPPHKRQKWWEKWFKKGE
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIMTDKRVRLTIKTIRAFLFMGALMLIFLSIVVMTVVLAAKWQGAPSIQVPRSTVLYDG
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
SGKQMGETHYGQKRYWVSLKNINPAVIDATLAVEDRNFFRHHGFDYMRMGGALIADLKAM
CCCCCCCCCCCCEEEEEEECCCCHHHEEEEEEECCCHHHHHCCCHHHHHCCHHHHHHHHH
SKVQGASTITQQYARNLYLGHDKTWKRKWNEAFYTIRLEQNYTKKDILEGYLNTIYYGHG
HHHCCHHHHHHHHHHHEECCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEECCC
AYGIEAASRLYFGKRAKDINTAEAAMLAGIPKGPSVYSPFVNEKKAKERQEMILTMMEKQ
CHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NKLTEMQAAALKKMPLRFEKQDKQTTEKTAPYFYDEAVKEISRLLRLTPEQAETGGYNVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEE
TTLNPRLQKIAEDTIEHTIDSSSDIQAGFAAINPSDGSVLALVGGRDYEKSPFNRVTQAM
EECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHH
RQPGSTMKPFLYYSAVQNGFTPATRMRSAETTFELGQGSAYSPSNYHGYYADGPITLLQA
HCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCHHCCCCCCCCCCCCCCCEECCCHHHHHHH
LALSDNIYAVKTHLFLGMDKLIDAAKQFGINSPLQKVPSLALGTSPVKPIEMVNGYAMFA
HHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHCCCCCCCHHHHHCCEEEEE
NGGKKIEPSFITKITDPNGKVLYEKPKRSKQILDPKAAFITANMMSGMFDKSLNGYTSVT
CCCCEECCHHHEEEECCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GRTIADQLTRRYAGKSGTTSTDSWMIGFYPGLASGVWTGYDKERTIDAVAEKNYAKQIWA
CHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EFMEKALEDAPAAALMPPEGVKGMYIDPATGYAAAPSCPSKYFAYFIKGTEPEQVCYGKE
HHHHHHHHCCCCEECCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
IYKEQNAGHEKPAAPPHKRQKWWEKWFKKGE
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]