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The map label for this gene is ywjE [H]

Identifier: 52787648

GI number: 52787648

Start: 3796880

End: 3798067

Strand: Direct

Name: ywjE [H]

Synonym: BLi03970

Alternate gene names: 52787648

Gene position: 3796880-3798067 (Clockwise)

Preceding gene: 52787640

Following gene: 52787653

Centisome position: 89.92

GC content: 48.23

Gene sequence:

>1188_bases
GTGCCGCTTATGATTATGGTATGTTTTCTCATCCTGCTTCTTGCGCTCGATTTCCATTTTGGCCGCAAAGCCTTTGAGAA
GAAAGCCTACGAGCCCGTTTTTTCAGAGAAGAAAAGCGATATTGAACTGATTCATAATGGAGAAGACTTGTGTGAGCGGC
TGCTGGATGACATCCGCCAGGCCGAATCGTCGGTGCATGTCATGTTTTATATTGTGAAGAACGATGACATCAGCCTTGAA
TTTTTGAAGGTGCTGAAGGATAAAGCGAAATCCGGCGTATGCGTACGATTGCTGATCGACCGGATCGGCGCGATGAAGGT
GAAGAAAAAAACGCTCTCCGGCCTGAAACAAAGCGGTGTGCACGTTTTTTTCGCCAACAAGCCGGGCTTCCCCTATTTCT
TTTACCGGCTGAATGCGAGGAATCATCGAAAAATCGCCGTCATAGACGGAAAGATCGGCTATGTGGGCGGATTTAACATC
GCGAAAGAATACCTTGGTAAAAAAGCGGAATTCGGACCGTGGAAGGATTACCATTTGCGAATGACGGGAGAAGGCGTTGC
CGACTTGCAGCACATTTTTATATCGGACTTCAAAAGAGAAGCGCCGCAGGCAAAGCCGGCCAACAGCGTATTTCCGCCGC
TGCAGCAAGGAGCTGTCACCCATACAACCCATGCGACAAAGGGATTTTCTCTAGAGGAAAAATACATTTCATTTATCGAA
CAGGCAAAAGAAAGAATCATGATTTGCACGCCGTACTATATTCCGAGCCCGGCTTTGCAGCAGGCTGTTCTCTCAGCCAG
GGAAAGGGGCGTGATCGTGTCGGTTCTCGTGCCGATGAAACCCGATCATCCGCTTGTCAAAGAGGCCGCCTACACGCACT
TTCCAGCCCTTTTAAAAGCGGGCTGCTATATTTACCGGTACTACAGAGGCTTTTATCATGCAAAAGCATTAATCGTCGAT
GACCGGCATGTGATGATCGGAACATCGAATTTTGACAACAGGAGCCTGTTTCTCAATGATGAAGTGAATGTCGTCATCCA
TGATAAAGACTGGACAAAGCAATTCTTCGACGTCGTCAAGGAAAGCATTGAACACGCCGAGCTTCTGACAAAGGAGCGGT
ATGCGAAGCGGCCGGTGATGCAGCGGCCCGTCGAATGGCTGGCGAAATCGATTTCATTCTTTTTATAA

Upstream 100 bases:

>100_bases
ATCTTAAATAATGAATGAGTGTTCAGTCAATATATTTTCATAAATGCAGGAAAATAGGGGGACACTATTCTTTACACGAA
AAAAGGGAGTGTCTTCATTG

Downstream 100 bases:

>100_bases
AACGTACGTTTATCCTGCATGACATGCGGGTAACATTTCACCAAAGTGATGATTACATACGGAGGGGATGTACGATGAAC
CAAGCAGAGTCGATTAAACT

Product: YwjE

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPLMIMVCFLILLLALDFHFGRKAFEKKAYEPVFSEKKSDIELIHNGEDLCERLLDDIRQAESSVHVMFYIVKNDDISLE
FLKVLKDKAKSGVCVRLLIDRIGAMKVKKKTLSGLKQSGVHVFFANKPGFPYFFYRLNARNHRKIAVIDGKIGYVGGFNI
AKEYLGKKAEFGPWKDYHLRMTGEGVADLQHIFISDFKREAPQAKPANSVFPPLQQGAVTHTTHATKGFSLEEKYISFIE
QAKERIMICTPYYIPSPALQQAVLSARERGVIVSVLVPMKPDHPLVKEAAYTHFPALLKAGCYIYRYYRGFYHAKALIVD
DRHVMIGTSNFDNRSLFLNDEVNVVIHDKDWTKQFFDVVKESIEHAELLTKERYAKRPVMQRPVEWLAKSISFFL

Sequences:

>Translated_395_residues
MPLMIMVCFLILLLALDFHFGRKAFEKKAYEPVFSEKKSDIELIHNGEDLCERLLDDIRQAESSVHVMFYIVKNDDISLE
FLKVLKDKAKSGVCVRLLIDRIGAMKVKKKTLSGLKQSGVHVFFANKPGFPYFFYRLNARNHRKIAVIDGKIGYVGGFNI
AKEYLGKKAEFGPWKDYHLRMTGEGVADLQHIFISDFKREAPQAKPANSVFPPLQQGAVTHTTHATKGFSLEEKYISFIE
QAKERIMICTPYYIPSPALQQAVLSARERGVIVSVLVPMKPDHPLVKEAAYTHFPALLKAGCYIYRYYRGFYHAKALIVD
DRHVMIGTSNFDNRSLFLNDEVNVVIHDKDWTKQFFDVVKESIEHAELLTKERYAKRPVMQRPVEWLAKSISFFL
>Mature_394_residues
PLMIMVCFLILLLALDFHFGRKAFEKKAYEPVFSEKKSDIELIHNGEDLCERLLDDIRQAESSVHVMFYIVKNDDISLEF
LKVLKDKAKSGVCVRLLIDRIGAMKVKKKTLSGLKQSGVHVFFANKPGFPYFFYRLNARNHRKIAVIDGKIGYVGGFNIA
KEYLGKKAEFGPWKDYHLRMTGEGVADLQHIFISDFKREAPQAKPANSVFPPLQQGAVTHTTHATKGFSLEEKYISFIEQ
AKERIMICTPYYIPSPALQQAVLSARERGVIVSVLVPMKPDHPLVKEAAYTHFPALLKAGCYIYRYYRGFYHAKALIVDD
RHVMIGTSNFDNRSLFLNDEVNVVIHDKDWTKQFFDVVKESIEHAELLTKERYAKRPVMQRPVEWLAKSISFFL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=360, Percent_Identity=30.2777777777778, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787007, Length=330, Percent_Identity=27.5757575757576, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45390; Mature: 45259

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLMIMVCFLILLLALDFHFGRKAFEKKAYEPVFSEKKSDIELIHNGEDLCERLLDDIRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCHHHHHHHHHHHHH
AESSVHVMFYIVKNDDISLEFLKVLKDKAKSGVCVRLLIDRIGAMKVKKKTLSGLKQSGV
HCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCC
HVFFANKPGFPYFFYRLNARNHRKIAVIDGKIGYVGGFNIAKEYLGKKAEFGPWKDYHLR
EEEEECCCCCCEEEEEECCCCCCEEEEEECCEEEECCHHHHHHHCCCCCCCCCCCCEEEE
MTGEGVADLQHIFISDFKREAPQAKPANSVFPPLQQGAVTHTTHATKGFSLEEKYISFIE
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEECCCCCCCCHHHHHHHHHH
QAKERIMICTPYYIPSPALQQAVLSARERGVIVSVLVPMKPDHPLVKEAAYTHFPALLKA
HHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GCYIYRYYRGFYHAKALIVDDRHVMIGTSNFDNRSLFLNDEVNVVIHDKDWTKQFFDVVK
CHHHHHHHHHHHHEEEEEEECCEEEEECCCCCCCEEEECCCEEEEEECCHHHHHHHHHHH
ESIEHAELLTKERYAKRPVMQRPVEWLAKSISFFL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLMIMVCFLILLLALDFHFGRKAFEKKAYEPVFSEKKSDIELIHNGEDLCERLLDDIRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCHHHHHHHHHHHHH
AESSVHVMFYIVKNDDISLEFLKVLKDKAKSGVCVRLLIDRIGAMKVKKKTLSGLKQSGV
HCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCC
HVFFANKPGFPYFFYRLNARNHRKIAVIDGKIGYVGGFNIAKEYLGKKAEFGPWKDYHLR
EEEEECCCCCCEEEEEECCCCCCEEEEEECCEEEECCHHHHHHHCCCCCCCCCCCCEEEE
MTGEGVADLQHIFISDFKREAPQAKPANSVFPPLQQGAVTHTTHATKGFSLEEKYISFIE
ECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEECCCCCCCCHHHHHHHHHH
QAKERIMICTPYYIPSPALQQAVLSARERGVIVSVLVPMKPDHPLVKEAAYTHFPALLKA
HHHHCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GCYIYRYYRGFYHAKALIVDDRHVMIGTSNFDNRSLFLNDEVNVVIHDKDWTKQFFDVVK
CHHHHHHHHHHHHEEEEEEECCEEEEECCCCCCCEEEECCCEEEEEECCHHHHHHHHHHH
ESIEHAELLTKERYAKRPVMQRPVEWLAKSISFFL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]