Click here to switch to the map view.

The map label for this gene is ywlF [H]

Identifier: 52787615

GI number: 52787615

Start: 3762126

End: 3762575

Strand: Reverse

Name: ywlF [H]

Synonym: BLi03937

Alternate gene names: 52787615

Gene position: 3762575-3762126 (Counterclockwise)

Preceding gene: 52787616

Following gene: 52787614

Centisome position: 89.1

GC content: 48.67

Gene sequence:

>450_bases
ATGAAAGTAATCATTGCATCAGATCACGGCGGAATCAATATCAGAAAAGAAATTATGGCATTGATGGACGAATTGAACAT
CGAATACGAAGATTACGGCTGCGAATGCGGTTCAGGATCTGTGGACTACCCGGATTATGCGTTTCCGGTCGCGCAAAAAG
TGGCGAACGGGGAAGCCGACAGAGGCATTTTAATATGCGGAACAGGAATCGGCATGAGCATTTCCGCCAATAAAGTAAAA
GGCATCCGCTGCGCCCTTGTCCACGACGTTTTCAGCGCCAAAATGACAAGAGAGCACAATGACACGAATGTTCTTGCGAT
GGGTGAACGGGTCATCGGCCCTGGTTTAGCGAGAGAGATTGCAAAGGTTTGGCTTACGACTGAGTTTTCAGCCGGGCGGC
ACGCCGTCCGCATCGGAAAAATCGCCGATTATGAAGACAAGCATGAATAA

Upstream 100 bases:

>100_bases
CAATAGACATGATCAATCAGAAGTATATAACGGCTGTATGCTGGCTTAACGGTTTCTGCGAGACTTTTGGCGGCACATTA
AACGAGGAGGAGTCATTCCA

Downstream 100 bases:

>100_bases
AGGGTGTCGTCAATCATGAAAAATCTTGAACAGACATGGCGTTCTATATTAGATGAATTTCATCAGCAAGCGAATTTGCG
CGAAGGCCACATCGTCGTCA

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKVIIASDHGGINIRKEIMALMDELNIEYEDYGCECGSGSVDYPDYAFPVAQKVANGEADRGILICGTGIGMSISANKVK
GIRCALVHDVFSAKMTREHNDTNVLAMGERVIGPGLAREIAKVWLTTEFSAGRHAVRIGKIADYEDKHE

Sequences:

>Translated_149_residues
MKVIIASDHGGINIRKEIMALMDELNIEYEDYGCECGSGSVDYPDYAFPVAQKVANGEADRGILICGTGIGMSISANKVK
GIRCALVHDVFSAKMTREHNDTNVLAMGERVIGPGLAREIAKVWLTTEFSAGRHAVRIGKIADYEDKHE
>Mature_149_residues
MKVIIASDHGGINIRKEIMALMDELNIEYEDYGCECGSGSVDYPDYAFPVAQKVANGEADRGILICGTGIGMSISANKVK
GIRCALVHDVFSAKMTREHNDTNVLAMGERVIGPGLAREIAKVWLTTEFSAGRHAVRIGKIADYEDKHE

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=146, Percent_Identity=45.8904109589041, Blast_Score=128, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 16229; Mature: 16229

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIIASDHGGINIRKEIMALMDELNIEYEDYGCECGSGSVDYPDYAFPVAQKVANGEAD
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
RGILICGTGIGMSISANKVKGIRCALVHDVFSAKMTREHNDTNVLAMGERVIGPGLAREI
CCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
AKVWLTTEFSAGRHAVRIGKIADYEDKHE
HHHHHCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKVIIASDHGGINIRKEIMALMDELNIEYEDYGCECGSGSVDYPDYAFPVAQKVANGEAD
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
RGILICGTGIGMSISANKVKGIRCALVHDVFSAKMTREHNDTNVLAMGERVIGPGLAREI
CCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
AKVWLTTEFSAGRHAVRIGKIADYEDKHE
HHHHHCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]