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The map label for this gene is ywnE [H]

Identifier: 52787573

GI number: 52787573

Start: 3714953

End: 3716419

Strand: Direct

Name: ywnE [H]

Synonym: BLi03895

Alternate gene names: 52787573

Gene position: 3714953-3716419 (Clockwise)

Preceding gene: 52787571

Following gene: 52787586

Centisome position: 87.98

GC content: 44.24

Gene sequence:

>1467_bases
ATGGATATTATTAAAAATATCAGTATCACATCGATATTTCTCGGTTTTATATTAGTTTTAAATACGCTTTTGGCAATCGT
TGTGATCTTTAGGGAACGCCGGGATGCAAGCTCCTCCTGGGCCTGGCTCTTGGTGCTCTTCTTTATTCCCGTTCTCGGCT
TTATCCTTTATTTGCTCTTCGGCCACAATTTGAGCAGGAATCACCTTTTTCAATGGGAAGACCGAAAGAAGATCGGGATT
GAAAAAATATTGGAACAGCAGCTGACCAAGCTGAAAAACCGCGAGTTTGAATTTCGCAATAAAGCGACGGCGGACAGCAA
AGACTTGATTTATATGCACATTTTGAATAACCACGCCGTTTTTACAGAAGACAATGCGGTCGAGCTGTTAACGGACGGAC
GCCAGAAATTCGACAGGCTGCTTCGCGATATTGAAAACGCCAAAGACCATATTCATCTTCAATATTATATTTATAAAGGC
GATGAGATCGGCAAAAAGCTCCGGGATGCGCTCATCAAAAAAGCAAAAGAAGGCGTTGAAGTCCGCGTTTTGTATGATGA
ATTAGGATCAAGAAGCCTCAGGAAAAGCTTTTTCAAAGAGCTGAAAAAAGCGGGAGGATTTGTCGAAGTCTTTTTCCCGT
CGCGCTTTAAATTTATCAATTTGCGCATGAATTACCGCAACCACCGCAAGCTCGTCATTATCGACGGCGTCATCGGCTAT
GTCGGAGGATTTAATGTCGGAGACGAATATCTCGGTCTGAACCCGGCCTTTGGCTATTGGCGGGACACGCACCTCAGAAT
CAAAGGAACAGCGGTCCACTCGATTCAAACGCGTTTTATCCTGGATTGGAATCAGGCCTCGCACCATCACGACATTACAT
ATATGCCAAACCATTTTCCTGACATCGACTCCCACGGAAACATCGGCATGCAAATCGTCACCAGCGGCCCCGATTCAGAA
TGGGAGCAGATTAAAAACGGCTATATTAAAATGATATCAACGGCAAAGCGGTCCATTCTTATTCAGACGCCTTATTTTAT
ACCGGACGCAAGCCTGCTCGATGCCCTCAGGATCGCTTGTCTGTCCGGCGTTGACGTCAGGATCATGATTCCTAACAAAC
CCGACCATCCGTTTGTGTACTGGGCAACCTATTCTTACATCGGCGAACTTTTAAAAGCAGGCGCCTCCGTATTTATTTAC
GACAACGGTTTTATCCATGCGAAAACGATTGTAGTAGATGAGGAAATTTCATCGGTCGGCACGGCAAATATTGATATGCG
CAGCTTCAAGCTCAATTTTGAAGTGAATGCATTTTTATATGATGAGGAAATTGCGAGAAGCCTTGTCCTATCATTTGAAA
AAGATCTGCAAGTGTCAAGGGAGCTGACCTTTGAGGAATATTTAAACAGAAGCAAATCGATCCGCTTCAAAGAAGCCATT
TCGATCCTGCTGTCGCCGATTTTATAG

Upstream 100 bases:

>100_bases
TTCCGGCCGCCGCCCCGTATAACATGAGATAGTTGCTTGAAAGGGGAGTAACTGTTATAAATGGAATGTATAAAAACAAA
TCCGTCTTGTGAGGTTCAAT

Downstream 100 bases:

>100_bases
CGCACAATTCATGCTGTTAAAAAAATCCTCCCGGAAACGGGAGGATTTTTGTTTTACTCACCAGAAGCTGCCGCACCTTT
GGGTGCTGAAGCATGCTTGT

Product: YwnE

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDIIKNISITSIFLGFILVLNTLLAIVVIFRERRDASSSWAWLLVLFFIPVLGFILYLLFGHNLSRNHLFQWEDRKKIGI
EKILEQQLTKLKNREFEFRNKATADSKDLIYMHILNNHAVFTEDNAVELLTDGRQKFDRLLRDIENAKDHIHLQYYIYKG
DEIGKKLRDALIKKAKEGVEVRVLYDELGSRSLRKSFFKELKKAGGFVEVFFPSRFKFINLRMNYRNHRKLVIIDGVIGY
VGGFNVGDEYLGLNPAFGYWRDTHLRIKGTAVHSIQTRFILDWNQASHHHDITYMPNHFPDIDSHGNIGMQIVTSGPDSE
WEQIKNGYIKMISTAKRSILIQTPYFIPDASLLDALRIACLSGVDVRIMIPNKPDHPFVYWATYSYIGELLKAGASVFIY
DNGFIHAKTIVVDEEISSVGTANIDMRSFKLNFEVNAFLYDEEIARSLVLSFEKDLQVSRELTFEEYLNRSKSIRFKEAI
SILLSPIL

Sequences:

>Translated_488_residues
MDIIKNISITSIFLGFILVLNTLLAIVVIFRERRDASSSWAWLLVLFFIPVLGFILYLLFGHNLSRNHLFQWEDRKKIGI
EKILEQQLTKLKNREFEFRNKATADSKDLIYMHILNNHAVFTEDNAVELLTDGRQKFDRLLRDIENAKDHIHLQYYIYKG
DEIGKKLRDALIKKAKEGVEVRVLYDELGSRSLRKSFFKELKKAGGFVEVFFPSRFKFINLRMNYRNHRKLVIIDGVIGY
VGGFNVGDEYLGLNPAFGYWRDTHLRIKGTAVHSIQTRFILDWNQASHHHDITYMPNHFPDIDSHGNIGMQIVTSGPDSE
WEQIKNGYIKMISTAKRSILIQTPYFIPDASLLDALRIACLSGVDVRIMIPNKPDHPFVYWATYSYIGELLKAGASVFIY
DNGFIHAKTIVVDEEISSVGTANIDMRSFKLNFEVNAFLYDEEIARSLVLSFEKDLQVSRELTFEEYLNRSKSIRFKEAI
SILLSPIL
>Mature_488_residues
MDIIKNISITSIFLGFILVLNTLLAIVVIFRERRDASSSWAWLLVLFFIPVLGFILYLLFGHNLSRNHLFQWEDRKKIGI
EKILEQQLTKLKNREFEFRNKATADSKDLIYMHILNNHAVFTEDNAVELLTDGRQKFDRLLRDIENAKDHIHLQYYIYKG
DEIGKKLRDALIKKAKEGVEVRVLYDELGSRSLRKSFFKELKKAGGFVEVFFPSRFKFINLRMNYRNHRKLVIIDGVIGY
VGGFNVGDEYLGLNPAFGYWRDTHLRIKGTAVHSIQTRFILDWNQASHHHDITYMPNHFPDIDSHGNIGMQIVTSGPDSE
WEQIKNGYIKMISTAKRSILIQTPYFIPDASLLDALRIACLSGVDVRIMIPNKPDHPFVYWATYSYIGELLKAGASVFIY
DNGFIHAKTIVVDEEISSVGTANIDMRSFKLNFEVNAFLYDEEIARSLVLSFEKDLQVSRELTFEEYLNRSKSIRFKEAI
SILLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=465, Percent_Identity=28.1720430107527, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1787007, Length=342, Percent_Identity=29.8245614035088, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56505; Mature: 56505

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIKNISITSIFLGFILVLNTLLAIVVIFRERRDASSSWAWLLVLFFIPVLGFILYLLF
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GHNLSRNHLFQWEDRKKIGIEKILEQQLTKLKNREFEFRNKATADSKDLIYMHILNNHAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEE
FTEDNAVELLTDGRQKFDRLLRDIENAKDHIHLQYYIYKGDEIGKKLRDALIKKAKEGVE
EECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCE
VRVLYDELGSRSLRKSFFKELKKAGGFVEVFFPSRFKFINLRMNYRNHRKLVIIDGVIGY
EEEEHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEEEECHHHH
VGGFNVGDEYLGLNPAFGYWRDTHLRIKGTAVHSIQTRFILDWNQASHHHDITYMPNHFP
CCCCCCCCHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCCCCEEECCCCCC
DIDSHGNIGMQIVTSGPDSEWEQIKNGYIKMISTAKRSILIQTPYFIPDASLLDALRIAC
CCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
LSGVDVRIMIPNKPDHPFVYWATYSYIGELLKAGASVFIYDNGFIHAKTIVVDEEISSVG
HCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCEEEEEECCEEEEEEEEEECHHHHCC
TANIDMRSFKLNFEVNAFLYDEEIARSLVLSFEKDLQVSRELTFEEYLNRSKSIRFKEAI
CCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
SILLSPIL
HHHHHCCC
>Mature Secondary Structure
MDIIKNISITSIFLGFILVLNTLLAIVVIFRERRDASSSWAWLLVLFFIPVLGFILYLLF
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GHNLSRNHLFQWEDRKKIGIEKILEQQLTKLKNREFEFRNKATADSKDLIYMHILNNHAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEE
FTEDNAVELLTDGRQKFDRLLRDIENAKDHIHLQYYIYKGDEIGKKLRDALIKKAKEGVE
EECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCE
VRVLYDELGSRSLRKSFFKELKKAGGFVEVFFPSRFKFINLRMNYRNHRKLVIIDGVIGY
EEEEHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEEEECHHHH
VGGFNVGDEYLGLNPAFGYWRDTHLRIKGTAVHSIQTRFILDWNQASHHHDITYMPNHFP
CCCCCCCCHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCCCCEEECCCCCC
DIDSHGNIGMQIVTSGPDSEWEQIKNGYIKMISTAKRSILIQTPYFIPDASLLDALRIAC
CCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
LSGVDVRIMIPNKPDHPFVYWATYSYIGELLKAGASVFIYDNGFIHAKTIVVDEEISSVG
HCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCEEEEEECCEEEEEEEEEECHHHHCC
TANIDMRSFKLNFEVNAFLYDEEIARSLVLSFEKDLQVSRELTFEEYLNRSKSIRFKEAI
CCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
SILLSPIL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]