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The map label for this gene is yvfI [H]

Identifier: 52787360

GI number: 52787360

Start: 3502617

End: 3503357

Strand: Reverse

Name: yvfI [H]

Synonym: BLi03676

Alternate gene names: 52787360

Gene position: 3503357-3502617 (Counterclockwise)

Preceding gene: 52787362

Following gene: 52787359

Centisome position: 82.97

GC content: 49.39

Gene sequence:

>741_bases
TTGAAATACAAACAAATCAAGACGAAAAAAATATATGAAGAAGTCGCGGAAGCGCTTCTTGAATCGATTAAAAGCGGAGA
GCTTGAGCCCGGCGACAAGCTTGACTCCGTCCAAGCGCTCGCCGACAGCTTTCAGGTCAGCCGTTCGGCCGTAAGGGAAG
CGCTGTCCGCGTTAAAAGCGATGGGGATGGTCGAAATGAAGCAGGGGGAAGGGACGTATGTGAAAAGGTTTGAGCCTGAG
CAAATTTCCATCCCCCTTTCGGCTGCACTTTTGATGAAAAAGAAAGATGTCGCTGAACTGCTCGAAGTCCGTAAAATACT
TGAAATCGGGGCAGTTTCCAGTGCGGCTCAAAAACGCACGGAGGATGATCTCGGACGCATGCAGGAAGCACTTGAGGATA
TGAAGCTGGCGGACGGGAACGGCGAACTTGGCGAAAAAGCCGACCTCGCGTTTCACCTTGCGCTTGCCGGCGCTTCGCAG
AACGATCTGCTGAAAGGCTTGATGAATCATGTGTCATCTCTCTTAATTGAGACGATGAGGGAGACGCGCAAAATTTGGCT
GTTCTCGAAGAATACAACTGTTAAGCGCCTTTATGAGGAACACGAAGCCATCTATCAGGCTGTGAAGGAAAAAGACGGCC
AAAAAGCGGAGCAAGCGATGCACAATCATCTGACAAATGTGGAAAATGTGCTGGCCTCATATTTTGAAGAAACGAAACAA
GCCGACGAGCCGGCTATTTAA

Upstream 100 bases:

>100_bases
CTTTTTTTGCATGGAGCCGAAGTCTATATGTTCCAGACGGAAAGAACGGTATAATAAAAAATAGCGCATCATGCCCAGTC
TGGTTACGAGGTGAAAAAAG

Downstream 100 bases:

>100_bases
GCAGCAGTCAGCTGCACGGCCTGAGTGAAAACGAAAAAGAAACCCCTTTCAAAAAATTTTGCCAAGGTGTAAGATGGAGA
AAGAGGGTTTTCTAACCAAG

Product: YvfI

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKYKQIKTKKIYEEVAEALLESIKSGELEPGDKLDSVQALADSFQVSRSAVREALSALKAMGMVEMKQGEGTYVKRFEPE
QISIPLSAALLMKKKDVAELLEVRKILEIGAVSSAAQKRTEDDLGRMQEALEDMKLADGNGELGEKADLAFHLALAGASQ
NDLLKGLMNHVSSLLIETMRETRKIWLFSKNTTVKRLYEEHEAIYQAVKEKDGQKAEQAMHNHLTNVENVLASYFEETKQ
ADEPAI

Sequences:

>Translated_246_residues
MKYKQIKTKKIYEEVAEALLESIKSGELEPGDKLDSVQALADSFQVSRSAVREALSALKAMGMVEMKQGEGTYVKRFEPE
QISIPLSAALLMKKKDVAELLEVRKILEIGAVSSAAQKRTEDDLGRMQEALEDMKLADGNGELGEKADLAFHLALAGASQ
NDLLKGLMNHVSSLLIETMRETRKIWLFSKNTTVKRLYEEHEAIYQAVKEKDGQKAEQAMHNHLTNVENVLASYFEETKQ
ADEPAI
>Mature_246_residues
MKYKQIKTKKIYEEVAEALLESIKSGELEPGDKLDSVQALADSFQVSRSAVREALSALKAMGMVEMKQGEGTYVKRFEPE
QISIPLSAALLMKKKDVAELLEVRKILEIGAVSSAAQKRTEDDLGRMQEALEDMKLADGNGELGEKADLAFHLALAGASQ
NDLLKGLMNHVSSLLIETMRETRKIWLFSKNTTVKRLYEEHEAIYQAVKEKDGQKAEQAMHNHLTNVENVLASYFEETKQ
ADEPAI

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=233, Percent_Identity=29.6137339055794, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790780, Length=233, Percent_Identity=30.0429184549356, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789353, Length=225, Percent_Identity=30.2222222222222, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1790032, Length=237, Percent_Identity=27.8481012658228, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789621, Length=252, Percent_Identity=27.3809523809524, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994955, Length=201, Percent_Identity=27.8606965174129, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082208, Length=240, Percent_Identity=28.3333333333333, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27456; Mature: 27456

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKQIKTKKIYEEVAEALLESIKSGELEPGDKLDSVQALADSFQVSRSAVREALSALKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMVEMKQGEGTYVKRFEPEQISIPLSAALLMKKKDVAELLEVRKILEIGAVSSAAQKRT
HHHHHEECCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDDLGRMQEALEDMKLADGNGELGEKADLAFHLALAGASQNDLLKGLMNHVSSLLIETMR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ETRKIWLFSKNTTVKRLYEEHEAIYQAVKEKDGQKAEQAMHNHLTNVENVLASYFEETKQ
HHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADEPAI
CCCCCC
>Mature Secondary Structure
MKYKQIKTKKIYEEVAEALLESIKSGELEPGDKLDSVQALADSFQVSRSAVREALSALKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMVEMKQGEGTYVKRFEPEQISIPLSAALLMKKKDVAELLEVRKILEIGAVSSAAQKRT
HHHHHEECCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDDLGRMQEALEDMKLADGNGELGEKADLAFHLALAGASQNDLLKGLMNHVSSLLIETMR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ETRKIWLFSKNTTVKRLYEEHEAIYQAVKEKDGQKAEQAMHNHLTNVENVLASYFEETKQ
HHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADEPAI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]