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The map label for this gene is blt [H]

Identifier: 52787129

GI number: 52787129

Start: 3281707

End: 3282903

Strand: Reverse

Name: blt [H]

Synonym: BLi03437

Alternate gene names: 52787129

Gene position: 3282903-3281707 (Counterclockwise)

Preceding gene: 52787130

Following gene: 52787127

Centisome position: 77.75

GC content: 52.72

Gene sequence:

>1197_bases
ATGAAAAAGATGGCCAGATTGTATATGCTCATGCTTAACGTGTTTATCGTGATGCTTGGTATAGGCTTGATTATTCCACT
CATGCCTACGTTTATAGAAGAATTCGGCGCGTCAGGCAGCACGCTCGGATTGCTGATCGCGGCTTCGGGGATTACCCAGC
TGCTGTTTTCGCCCGTTGCCGGGGAAATGACTGATAAATACGGAAGGCGGAAAATGATCATTTTAGGAATCGGTGCGTTT
GCGGTTTCCCAGCTGCTTTTCGCCTTGGCGAGCCAGATGTGGCTGCTGTTTGTTTCCCGCCTTCTCGGCGGAGCGGGAGC
TGCGTTTTTGGTGCCGGCCATGTTTGCCTACATCGCTGACATCACATCGGAAAAGGATCGAAGCAAAGGGATGGGGCTGA
TCAGCGCCGCGATGTCTCTCGGCTTTGTCATCGGTCCGGGAGCAGGCGGATATTTGGCGGCGTTCGGGCTCACCTTCCCG
TTTTACGTGTCAGCCGGATTAGCCGGCTTGGCGACGGTTTTATCGCTTCTTGTCCTCCCGGAAACGCTCAGTCAAGAAAA
GATGCTTGAAAAGCGCCGCTCCGTTCAAAAGCGCGAACCGCTCGCTAAGCAAATGGCGCGCGCGCTGCGGTCGCCGTATG
CATTTCTGTTTATCCTCGTGTTCATCTTGAACTTCGGGATCATGAATTTCGAGGCGGTATTCAGCCTTTATGTCGATCAT
AAGCACGGCTTTACACCGGGAGACATTGCCTTTGTCATAACAGCGGCAAGTCTGATCGGCGTGTTCGTCCAGGCGGTGGC
TCTCGGGATGCTGACGAACCGCTTCGGCGAAAAAAAGCTGATGAACATGACGCTGATCGGTTCTGCCGGAGCGCTTGCCG
TCTGCTCGATTGCCGGTTCGTACTGGCTGGTTTTCGGGGCAACAATTGTTTTCTTCATGCTGACGTCCATTCTTCGACCG
GCGATTAATACGCTGATTTCAAAAATGGCCGGAGATGAACAGGGATTTGCCGCAGGCATGAACAATGCGTTTATGAGCCT
TGCCAACATTGTCGGACCGACAGTGGCCGGTCTGTTATTCGATGTGAACGTAGAAATTCCATATATTTTTGGTGTGGTCG
TTCTCGTGCTCAGCTTTTTGGCGGCAGTCAGCTGGGGGCGGAAGCAGCAGGCGCGCACAATTCCTGAAAAAGTATAA

Upstream 100 bases:

>100_bases
AAAAAACTAGACTTTAAACCTATCTGTGGATATACTAATTTAGTAATTGTTCGAATTTAAGATTGTCCGGTATTTTCGGG
CTGAAGGAAGGTATCCGCAG

Downstream 100 bases:

>100_bases
GGGAGCCGGCACATATGCCGGCTTTTTTATGGTGAAATGCTCAAATCATCAAATATGCCGGCGCGGCTTTACATCCTCCG
ATTGCCGCGGGGAAGGGATT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKKMARLYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVAGEMTDKYGRRKMIILGIGAF
AVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIADITSEKDRSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFP
FYVSAGLAGLATVLSLLVLPETLSQEKMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDH
KHGFTPGDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLIGSAGALAVCSIAGSYWLVFGATIVFFMLTSILRP
AINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLFDVNVEIPYIFGVVVLVLSFLAAVSWGRKQQARTIPEKV

Sequences:

>Translated_398_residues
MKKMARLYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVAGEMTDKYGRRKMIILGIGAF
AVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIADITSEKDRSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFP
FYVSAGLAGLATVLSLLVLPETLSQEKMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDH
KHGFTPGDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLIGSAGALAVCSIAGSYWLVFGATIVFFMLTSILRP
AINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLFDVNVEIPYIFGVVVLVLSFLAAVSWGRKQQARTIPEKV
>Mature_398_residues
MKKMARLYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVAGEMTDKYGRRKMIILGIGAF
AVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIADITSEKDRSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFP
FYVSAGLAGLATVLSLLVLPETLSQEKMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDH
KHGFTPGDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLIGSAGALAVCSIAGSYWLVFGATIVFFMLTSILRP
AINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLFDVNVEIPYIFGVVVLVLSFLAAVSWGRKQQARTIPEKV

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=336, Percent_Identity=27.9761904761905, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI94721311, Length=408, Percent_Identity=26.9607843137255, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI217416366, Length=409, Percent_Identity=23.7163814180929, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI118582257, Length=145, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787291, Length=401, Percent_Identity=25.1870324189526, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081738, Length=216, Percent_Identity=28.7037037037037, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71996331, Length=373, Percent_Identity=26.0053619302949, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17551570, Length=166, Percent_Identity=33.7349397590361, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357569, Length=357, Percent_Identity=28.0112044817927, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI161080926, Length=379, Percent_Identity=27.1767810026385, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161080924, Length=379, Percent_Identity=27.1767810026385, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656463, Length=380, Percent_Identity=27.6315789473684, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656468, Length=380, Percent_Identity=27.6315789473684, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17738085, Length=402, Percent_Identity=25.8706467661692, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221513297, Length=399, Percent_Identity=24.3107769423559, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137160, Length=189, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078541, Length=165, Percent_Identity=32.1212121212121, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62471713, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471717, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471715, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42631; Mature: 42631

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMARLYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GEMTDKYGRRKMIILGIGAFAVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIAD
HHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ITSEKDRSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFPFYVSAGLAGLATVLSLLVLP
HHCCHHHHHCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLSQEKMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDH
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
KHGFTPGDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLIGSAGALAVCSIAGS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEECCCCHHHHHHHHHH
YWLVFGATIVFFMLTSILRPAINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DVNVEIPYIFGVVVLVLSFLAAVSWGRKQQARTIPEKV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure
MKKMARLYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GEMTDKYGRRKMIILGIGAFAVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIAD
HHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ITSEKDRSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFPFYVSAGLAGLATVLSLLVLP
HHCCHHHHHCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLSQEKMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDH
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
KHGFTPGDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLIGSAGALAVCSIAGS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEECCCCHHHHHHHHHH
YWLVFGATIVFFMLTSILRPAINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DVNVEIPYIFGVVVLVLSFLAAVSWGRKQQARTIPEKV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]