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The map label for this gene is mrpD [H]

Identifier: 52787043

GI number: 52787043

Start: 3201984

End: 3203471

Strand: Direct

Name: mrpD [H]

Synonym: BLi03348

Alternate gene names: 52787043

Gene position: 3201984-3203471 (Clockwise)

Preceding gene: 52787042

Following gene: 52787044

Centisome position: 75.83

GC content: 49.4

Gene sequence:

>1488_bases
ATGATCAACATGAATAATCTTGTAATTCTGCCAATACTGATTCCTTTAATGGCCGCAATTCTGCTTATCTTTATGAACAG
GTCGATCGTGCTGATGCGTGCATTCAGCGCCATATCTTCCATTGCGGCGATCGCTGCGGCCGCCGTTCTTGTGCAGACAG
TCTATACGGACGGAATCCAGACCTTGTATCTCGGCGGGTGGAAGCCTCCGTTCGGAATCAGCCTTGTCGCTGATCAATTT
GCCAGCCTTCTGGCGTTAACGACAGCCATTATCGGATTTCTGACGGTTCTTTATTCGTTTCGCTCCATCGGCGAAAAACG
CGAGCGTTTCTTTTATTATCCCGCCGTCCAGTTCCTGCTTGCCGGAGTTAGCGGCGCTTTCTTGACCGGGGACTTGTTTA
ACCTGTTCGTATTTTTTGAGGTGCTTCTGATGGCTTCGTATGTGCTGATCGTCATCGGCGGCACGAAGATTCAGCTGCGG
GAGTCTCTGAAGTACATCGTCTTTAACATTATTTCTTCCGCCCTCTTTGTCATCGGAGTCGCTTATTTATATGCGGTTAC
GGGCACATTAAACATGGCGGATCTGAGCGTCCGGATCAGCGAATCAGGGCAAACCGGGCTGATCACGGTCATCGCTGTTT
TATTTTTGATCGTCTTCGGTTTAAAAGGCGGCATCTTCCCGCTGTATTTCTGGATGCCTGGTTCCTATTATGCGCCGCCA
GCCGCTGTTTCCGCACTGTTTGGCGCACTTCTGACCAAAGTCGGCCTGTACGCGATCACAAGGGTGTTTACATTAATTTT
CACCCAAGACGCCGCTTTTACTCATCAATTAATGGTCTGGCTTGCGGCGCTTACGATCATCTTCGGGGTCATCGGCTCGA
TCGCCTACTGGGATGTTCAAAAAATCATCATCTACAATATTGTCACTGCGGTCGGCGTCATCTTGTTCGGCATCGCCGCC
AATACGCCGGCTTCAATCGAAGGATCTGTCTACTATTTGATTCATGACATGATCATTAAAGGAGCGCTGTTTATGCTTGG
CGGAGCGCTTTTTGCCCTGACAGGGACAAACAACCTGAAAAAAATGAGCGGCCTGATCAAAAACCATCCCGTGCTCGGCT
GGATGTTTATGATTTCAGCCGTTTCATTGGCAGGTGTTCCTCCTTTCAGCGGATTCATCGGCAAGCTGAAAATTGCAGAA
GGCGGCTTTTTAAGCGGCGAATTCGTGATTACGCTGCTGATGCTTTTATCCAGCCTGCTCGTCTTGTATTCCGTCATGAA
AATATTTATCAACGGCTTCTGGGGGGATGAACAGGAAGATGCTCCGGCCAAAAGGCAGCTAAAAGGCTACATGTATCCGG
CAGCGGTACTGCTCTTTCTGTCGCTTGCCATCGGACTCGGAACCGAGCTTATCGCTCCGTATTTTCACCAGGCGGCTGAT
ACGCTCGTCAACCCCGAAAAATATATTGAAGCCGTTTTGAAGGAGTAG

Upstream 100 bases:

>100_bases
GCGATCGTCATTGCTTTCGGGGTTACCTCGTTTCTCCTGGTCATGGCTTTCCGCGCCTATCAAGAGCTGAAAATGGATGA
TATGGATCAAATGAGGGGAA

Downstream 100 bases:

>100_bases
ATCGCATGGCATTACAAATATTGTTGAACTTTATTCTTGCGTTGTGCTGGATGTTTCTGAACAATGACTACACCGCTGCC
GGGTTTGTCATCGGTTATGT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MINMNNLVILPILIPLMAAILLIFMNRSIVLMRAFSAISSIAAIAAAAVLVQTVYTDGIQTLYLGGWKPPFGISLVADQF
ASLLALTTAIIGFLTVLYSFRSIGEKRERFFYYPAVQFLLAGVSGAFLTGDLFNLFVFFEVLLMASYVLIVIGGTKIQLR
ESLKYIVFNIISSALFVIGVAYLYAVTGTLNMADLSVRISESGQTGLITVIAVLFLIVFGLKGGIFPLYFWMPGSYYAPP
AAVSALFGALLTKVGLYAITRVFTLIFTQDAAFTHQLMVWLAALTIIFGVIGSIAYWDVQKIIIYNIVTAVGVILFGIAA
NTPASIEGSVYYLIHDMIIKGALFMLGGALFALTGTNNLKKMSGLIKNHPVLGWMFMISAVSLAGVPPFSGFIGKLKIAE
GGFLSGEFVITLLMLLSSLLVLYSVMKIFINGFWGDEQEDAPAKRQLKGYMYPAAVLLFLSLAIGLGTELIAPYFHQAAD
TLVNPEKYIEAVLKE

Sequences:

>Translated_495_residues
MINMNNLVILPILIPLMAAILLIFMNRSIVLMRAFSAISSIAAIAAAAVLVQTVYTDGIQTLYLGGWKPPFGISLVADQF
ASLLALTTAIIGFLTVLYSFRSIGEKRERFFYYPAVQFLLAGVSGAFLTGDLFNLFVFFEVLLMASYVLIVIGGTKIQLR
ESLKYIVFNIISSALFVIGVAYLYAVTGTLNMADLSVRISESGQTGLITVIAVLFLIVFGLKGGIFPLYFWMPGSYYAPP
AAVSALFGALLTKVGLYAITRVFTLIFTQDAAFTHQLMVWLAALTIIFGVIGSIAYWDVQKIIIYNIVTAVGVILFGIAA
NTPASIEGSVYYLIHDMIIKGALFMLGGALFALTGTNNLKKMSGLIKNHPVLGWMFMISAVSLAGVPPFSGFIGKLKIAE
GGFLSGEFVITLLMLLSSLLVLYSVMKIFINGFWGDEQEDAPAKRQLKGYMYPAAVLLFLSLAIGLGTELIAPYFHQAAD
TLVNPEKYIEAVLKE
>Mature_495_residues
MINMNNLVILPILIPLMAAILLIFMNRSIVLMRAFSAISSIAAIAAAAVLVQTVYTDGIQTLYLGGWKPPFGISLVADQF
ASLLALTTAIIGFLTVLYSFRSIGEKRERFFYYPAVQFLLAGVSGAFLTGDLFNLFVFFEVLLMASYVLIVIGGTKIQLR
ESLKYIVFNIISSALFVIGVAYLYAVTGTLNMADLSVRISESGQTGLITVIAVLFLIVFGLKGGIFPLYFWMPGSYYAPP
AAVSALFGALLTKVGLYAITRVFTLIFTQDAAFTHQLMVWLAALTIIFGVIGSIAYWDVQKIIIYNIVTAVGVILFGIAA
NTPASIEGSVYYLIHDMIIKGALFMLGGALFALTGTNNLKKMSGLIKNHPVLGWMFMISAVSLAGVPPFSGFIGKLKIAE
GGFLSGEFVITLLMLLSSLLVLYSVMKIFINGFWGDEQEDAPAKRQLKGYMYPAAVLLFLSLAIGLGTELIAPYFHQAAD
TLVNPEKYIEAVLKE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=305, Percent_Identity=20.983606557377, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI251831116, Length=317, Percent_Identity=21.7665615141956, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788613, Length=367, Percent_Identity=27.7929155313352, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788829, Length=425, Percent_Identity=25.4117647058824, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788827, Length=455, Percent_Identity=22.6373626373626, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788831, Length=350, Percent_Identity=24.5714285714286, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI2367154, Length=351, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788614, Length=488, Percent_Identity=24.3852459016393, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693160, Length=308, Percent_Identity=24.6753246753247, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 53765; Mature: 53765

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINMNNLVILPILIPLMAAILLIFMNRSIVLMRAFSAISSIAAIAAAAVLVQTVYTDGIQ
CCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLYLGGWKPPFGISLVADQFASLLALTTAIIGFLTVLYSFRSIGEKRERFFYYPAVQFLL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
AGVSGAFLTGDLFNLFVFFEVLLMASYVLIVIGGTKIQLRESLKYIVFNIISSALFVIGV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYLYAVTGTLNMADLSVRISESGQTGLITVIAVLFLIVFGLKGGIFPLYFWMPGSYYAPP
HHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
AAVSALFGALLTKVGLYAITRVFTLIFTQDAAFTHQLMVWLAALTIIFGVIGSIAYWDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIIIYNIVTAVGVILFGIAANTPASIEGSVYYLIHDMIIKGALFMLGGALFALTGTNNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHH
KMSGLIKNHPVLGWMFMISAVSLAGVPPFSGFIGKLKIAEGGFLSGEFVITLLMLLSSLL
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
VLYSVMKIFINGFWGDEQEDAPAKRQLKGYMYPAAVLLFLSLAIGLGTELIAPYFHQAAD
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TLVNPEKYIEAVLKE
HHCCHHHHHHHHHCC
>Mature Secondary Structure
MINMNNLVILPILIPLMAAILLIFMNRSIVLMRAFSAISSIAAIAAAAVLVQTVYTDGIQ
CCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLYLGGWKPPFGISLVADQFASLLALTTAIIGFLTVLYSFRSIGEKRERFFYYPAVQFLL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
AGVSGAFLTGDLFNLFVFFEVLLMASYVLIVIGGTKIQLRESLKYIVFNIISSALFVIGV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYLYAVTGTLNMADLSVRISESGQTGLITVIAVLFLIVFGLKGGIFPLYFWMPGSYYAPP
HHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
AAVSALFGALLTKVGLYAITRVFTLIFTQDAAFTHQLMVWLAALTIIFGVIGSIAYWDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIIIYNIVTAVGVILFGIAANTPASIEGSVYYLIHDMIIKGALFMLGGALFALTGTNNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHH
KMSGLIKNHPVLGWMFMISAVSLAGVPPFSGFIGKLKIAEGGFLSGEFVITLLMLLSSLL
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
VLYSVMKIFINGFWGDEQEDAPAKRQLKGYMYPAAVLLFLSLAIGLGTELIAPYFHQAAD
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TLVNPEKYIEAVLKE
HHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]