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The map label for this gene is gbsB [H]

Identifier: 52786969

GI number: 52786969

Start: 3134376

End: 3135584

Strand: Reverse

Name: gbsB [H]

Synonym: BLi03269

Alternate gene names: 52786969

Gene position: 3135584-3134376 (Counterclockwise)

Preceding gene: 52786970

Following gene: 52786968

Centisome position: 74.26

GC content: 51.12

Gene sequence:

>1209_bases
ATGACTTTAAATATGAAAGTAGAAGCGATGAACAAATTCCACACGTTTGAAATCCCTACCGTCATTAAGCACGGAATCGG
AGCCGTTAAACATGTGGGGGAAGAAGTGAAAAAGCTTGGCGTTTCAAAAGCGCTGCTTGTCACAGACCCTGGCATCTACA
AAGCCGGTGTAACAAACCCGGTCGTTGTATCCCTTCAGGAAGCAGGCGTAGAAGTTGCCGTCTTTAATAAAGTCGAGCCG
AATCCGCCTGTCCGTCTTGTCGCTGAAGGTTCCAAACTGTATGCTGAAGAAAACTGCGACGGCCTTGTCGCGGTCGGCGG
CGGAAGCTCAATGGATACGGCGAAAGCGATCGGAGTTGAAGCCACTCATGAAGGCTCAGTCCTTGACTATGAAGCGGCAG
AAGGAAAAAAGCCGCTTGAAAACCGGATTCCTCCGCTTACAACGATCCCGACGACTGCCGGAACCGGCTCAGAAGTTACA
CAATGGGCGGTTATCACAGATGAAGAGCGCGAATTTAAATTCAATACGGGCGGCCCGCTGATTGCTGCTCATTTGACAAT
TATCGATCCTGAATTGCATGTATCAATGCCTCCGCACGTAACAGCGATGACCGGCGTTGACGCTTTGGCTCATGCCATCG
AGTGCTATACGATGAAATTTGCCCAGCCGATTACTGACGCGGTGGCCCTTCTTGCGATCGAATACGGCGCACATTACATC
CGCAGAGCGTTTGCCGATGGAGAAGATCTTGAAGCGAGATACGGCATGGCGCAGGCGGCCATGCTTGCAGGCCTTTCTTA
CGGAAGCGAATCCGCAGGCGCTGCTCACGCGATGAGCCAGACGCTCGGAGGGATCATCCCTGTCGCGCACGGCCAATGTG
TGGCGGCGATGATGGGCCCTGTAATGGAATACAACTGGAAAGGCTACCCTGAAAAATTTGCCCGCATTGCAAAAGCGTTC
GGAATTGACACAAGCGGCATGTCTGCAGTTGAAGCGGCTAAAGCTGCGGTGAACTGGATGTATCAGCTTGTGGAAGACTT
AGAGATTCCTAGTCTTGAAGAGCAAGGCGTATCACCTGACATGATCGACCGCCTTTCAAAAGAAGCGATGAAAGATCCGC
AAACGGTCGGTAACCCTAGAGACCTGAACGAAAAAGCGTACAATTGGATTTACAAACGCTGCTTCAATTTAACCCCGAAA
ACTGTCTGA

Upstream 100 bases:

>100_bases
GCATGGGCCTTGAAGAATACACAGAAGTGAAGCATGTCTACCGCAATACGAAACCGGCGGCTGTCAATTGGTTTAAAGCA
TAAGAAGGAGGAGACTATTC

Downstream 100 bases:

>100_bases
AAAAATTGAAAAAGCCCGTTGTGTGCAGCACACAGCGGGCTTTTTCAATTTGAGAAAAAAAGGGAGGCAATGTAAAATAA
ATCACATCTTGAAAAAAAGG

Product: GbsB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTLNMKVEAMNKFHTFEIPTVIKHGIGAVKHVGEEVKKLGVSKALLVTDPGIYKAGVTNPVVVSLQEAGVEVAVFNKVEP
NPPVRLVAEGSKLYAEENCDGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVT
QWAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGVDALAHAIECYTMKFAQPITDAVALLAIEYGAHYI
RRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAF
GIDTSGMSAVEAAKAAVNWMYQLVEDLEIPSLEEQGVSPDMIDRLSKEAMKDPQTVGNPRDLNEKAYNWIYKRCFNLTPK
TV

Sequences:

>Translated_402_residues
MTLNMKVEAMNKFHTFEIPTVIKHGIGAVKHVGEEVKKLGVSKALLVTDPGIYKAGVTNPVVVSLQEAGVEVAVFNKVEP
NPPVRLVAEGSKLYAEENCDGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVT
QWAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGVDALAHAIECYTMKFAQPITDAVALLAIEYGAHYI
RRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAF
GIDTSGMSAVEAAKAAVNWMYQLVEDLEIPSLEEQGVSPDMIDRLSKEAMKDPQTVGNPRDLNEKAYNWIYKRCFNLTPK
TV
>Mature_401_residues
TLNMKVEAMNKFHTFEIPTVIKHGIGAVKHVGEEVKKLGVSKALLVTDPGIYKAGVTNPVVVSLQEAGVEVAVFNKVEPN
PPVRLVAEGSKLYAEENCDGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVTQ
WAVITDEEREFKFNTGGPLIAAHLTIIDPELHVSMPPHVTAMTGVDALAHAIECYTMKFAQPITDAVALLAIEYGAHYIR
RAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAFG
IDTSGMSAVEAAKAAVNWMYQLVEDLEIPSLEEQGVSPDMIDRLSKEAMKDPQTVGNPRDLNEKAYNWIYKRCFNLTPKT
V

Specific function: Essential for the utilization of choline as a precursor [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=363, Percent_Identity=30.0275482093664, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI48994951, Length=380, Percent_Identity=35, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789163, Length=358, Percent_Identity=38.5474860335196, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082107, Length=391, Percent_Identity=31.7135549872123, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1787493, Length=393, Percent_Identity=29.7709923664122, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1789386, Length=403, Percent_Identity=26.302729528536, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=421, Percent_Identity=25.8907363420428, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321181, Length=397, Percent_Identity=33.7531486146096, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24657991, Length=442, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 43105; Mature: 42974

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNMKVEAMNKFHTFEIPTVIKHGIGAVKHVGEEVKKLGVSKALLVTDPGIYKAGVTNP
CEEEEEEHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCC
VVVSLQEAGVEVAVFNKVEPNPPVRLVAEGSKLYAEENCDGLVAVGGGSSMDTAKAIGVE
EEEEEHHCCEEEEEEECCCCCCCEEEEECCCEEECCCCCCEEEEECCCCCCCHHHHHCCE
ATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREFKFNTGGPL
ECCCCCEEEECCCCCCCCHHHCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEECCCCCE
IAAHLTIIDPELHVSMPPHVTAMTGVDALAHAIECYTMKFAQPITDAVALLAIEYGAHYI
EEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHH
VMEYNWKGYPEKFARIAKAFGIDTSGMSAVEAAKAAVNWMYQLVEDLEIPSLEEQGVSPD
HHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
MIDRLSKEAMKDPQTVGNPRDLNEKAYNWIYKRCFNLTPKTV
HHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLNMKVEAMNKFHTFEIPTVIKHGIGAVKHVGEEVKKLGVSKALLVTDPGIYKAGVTNP
EEEEEEHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCC
VVVSLQEAGVEVAVFNKVEPNPPVRLVAEGSKLYAEENCDGLVAVGGGSSMDTAKAIGVE
EEEEEHHCCEEEEEEECCCCCCCEEEEECCCEEECCCCCCEEEEECCCCCCCHHHHHCCE
ATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREFKFNTGGPL
ECCCCCEEEECCCCCCCCHHHCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEECCCCCE
IAAHLTIIDPELHVSMPPHVTAMTGVDALAHAIECYTMKFAQPITDAVALLAIEYGAHYI
EEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGP
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHH
VMEYNWKGYPEKFARIAKAFGIDTSGMSAVEAAKAAVNWMYQLVEDLEIPSLEEQGVSPD
HHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
MIDRLSKEAMKDPQTVGNPRDLNEKAYNWIYKRCFNLTPKTV
HHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377 [H]