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The map label for this gene is glgB

Identifier: 52786954

GI number: 52786954

Start: 3113595

End: 3115478

Strand: Reverse

Name: glgB

Synonym: BLi03230

Alternate gene names: 52786954

Gene position: 3115478-3113595 (Counterclockwise)

Preceding gene: 52786955

Following gene: 52786953

Centisome position: 73.78

GC content: 46.92

Gene sequence:

>1884_bases
ATGGCTGGTGTGAGTGCCTCGGATATTAAAGCGCATCTCTTTCATGAAGGTCGGCTTTATGAAAGCTACAGGTTCTTCGG
CGCTCATCAGACGGAACAGGACGGCCGCCAAGGCTTCCGTTTCTGTGTTTGGGCCCCGCATGCGAAGGAGGTTCGAATCG
CCGGAACGTTTAACGGCTGGTCCGGTGTCCTGCACCGAATGGAAAAGCGTCATCAGGAAGGCATATGGGAACTGTTTATC
CCCGGTATTGGAGAAGGGGAGCTGTATAAATATGAAATGATTACAGCCGCAAATGAAACGAAATTGAAGGCTGATCCTTA
TGCTTTTTTTTCTGAAGTGAGGCCGAAAACGGCATCGGTCACTTATCAATTGGCCGGATACCAATGGGGCGACCGGAAGT
GGCAAAAGAAAAAGCAGCAAAAAGCCGTGTATGAAAAACCGATTTCCATCTACGAGCTTCATATCGGTTCGTGGAAAAAG
AAAGCGGATGGACGTTTTTTCACATACCGGGAACTGAGCGAAACGGTCATTCCTTACGTCAAAGAACACGGATTCACACA
TATCGAGCTGATGCCGCTGACAGAGCACCCGTTTGACCGTTCATGGGGCTACCAGACAACAGGGTACTACAGTCCGACGA
GCAGATATGGCGAACCGCATGACTTGATGTATTTCATCGACCAGTGCCATCAGCATGAGATCGGCGTCATTATGGATTGG
GTTTCCGGCCATTTTTGCAAAGATGACCACGGCTTGTATTTGTTTGACGGTATGCCTCTGTATGAATATAAACACGAGCA
TGACAGGGAAAATTGGGAATGGGGGACCGCCAATTTTGACCTCGGAAAACGAGAAGTTCACAGTTTTTTAATCTCGAATG
CGTTGTATTGGATCGAGATGTATCATATAGATGGATTTCGAGTCGATGCGGTTGCGAACCTCCTGTATTGGCCAAACCGT
CCTCAGCCTGAAGCGAACGGCTTTGCGATCGAATTTATTCAAACGTTAAATGAACAGGTTTTTGCAAAAGATCCCCACTT
TTTGATGATTGCAGAGGACTCCACAGACTGGCCGCTTGTGACGCATCCGACCTATGAAGGCGGTCTCGGGTTTAATTATA
AGTGGAATATGGGTTGGATGAACGATGTATTAACCTATATGGAAGCTTCCCCAGAACAAAGAAAACACCTGCATCATCTT
GTTTCATTCTCCTTAATGTATGCCTACTCAGAAAATTACATCCTGCCGTTCTCCCACGATGAAGTGGTGTACCGCAAAAA
GTCTCTGCTTCATAAGATGCCCGGCGATTATTGGCAGAAATTCGCCCAGTACCGCCTGCTGATCAGTTATTTTATGATGC
ACCCGGGAAAAAAGCTTCTTTTTATGGGCGGTGAGTTTGCTCAATTTGATGAATGGAAAGACGAAGAAGAGCTCGATTGG
TTTTTGGACGATTTTGACATGCACCGCAAAGCCCGAATGTTTACAAAGGAGGCTCTACACCTCTACAAAAAAAGCCGTAT
CCTGTATGAAAATGATCATCGGCAGCAAGGATTCGAGTGGATTGACGTTAACAATGCGGAACAGTCGATTGTATCGTTTA
TCCGCTATGGAAAACAGCCCGGTGAAGCGCTGATTATCGTCTGCAATTTCACGCCTTCCGTCTATCACGAATATCGGGTC
GGCGTCCCTTTTCAAACCGAATATATCGAAGTGCTTAACAGCGATGATGCAAAATACGGAGGTTCACACCAAATCAACCC
AAAACGCCTTCAGGCAAGAAAAGGCGTTCTGCACGGCAAACCATACAGCATCAGCATGACAGTCCCTCCGCTGGGAGCTG
CGGTTTTCAGAGCAGTCAAAAAGAGAGGAGCGAAAAAGGGATGA

Upstream 100 bases:

>100_bases
ACTTTGTCAATAAAAAGAAGCGCTTTCATTAAAGGGATCAAGCCATATTATCCGAATAAAAGAGTATTCTTTAAATATAG
TGGCAGAAAGGATGAAATCA

Downstream 100 bases:

>100_bases
AAGAACAATGTGTGGCTATGCTTTTGGCAGGCGGCAAGGGAAGCAGGCTGAATGCCTTAACCAAAAACCTCGCAAAACCC
GCCGTTCCATTTGGCGGCAA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MAGVSASDIKAHLFHEGRLYESYRFFGAHQTEQDGRQGFRFCVWAPHAKEVRIAGTFNGWSGVLHRMEKRHQEGIWELFI
PGIGEGELYKYEMITAANETKLKADPYAFFSEVRPKTASVTYQLAGYQWGDRKWQKKKQQKAVYEKPISIYELHIGSWKK
KADGRFFTYRELSETVIPYVKEHGFTHIELMPLTEHPFDRSWGYQTTGYYSPTSRYGEPHDLMYFIDQCHQHEIGVIMDW
VSGHFCKDDHGLYLFDGMPLYEYKHEHDRENWEWGTANFDLGKREVHSFLISNALYWIEMYHIDGFRVDAVANLLYWPNR
PQPEANGFAIEFIQTLNEQVFAKDPHFLMIAEDSTDWPLVTHPTYEGGLGFNYKWNMGWMNDVLTYMEASPEQRKHLHHL
VSFSLMYAYSENYILPFSHDEVVYRKKSLLHKMPGDYWQKFAQYRLLISYFMMHPGKKLLFMGGEFAQFDEWKDEEELDW
FLDDFDMHRKARMFTKEALHLYKKSRILYENDHRQQGFEWIDVNNAEQSIVSFIRYGKQPGEALIIVCNFTPSVYHEYRV
GVPFQTEYIEVLNSDDAKYGGSHQINPKRLQARKGVLHGKPYSISMTVPPLGAAVFRAVKKRGAKKG

Sequences:

>Translated_627_residues
MAGVSASDIKAHLFHEGRLYESYRFFGAHQTEQDGRQGFRFCVWAPHAKEVRIAGTFNGWSGVLHRMEKRHQEGIWELFI
PGIGEGELYKYEMITAANETKLKADPYAFFSEVRPKTASVTYQLAGYQWGDRKWQKKKQQKAVYEKPISIYELHIGSWKK
KADGRFFTYRELSETVIPYVKEHGFTHIELMPLTEHPFDRSWGYQTTGYYSPTSRYGEPHDLMYFIDQCHQHEIGVIMDW
VSGHFCKDDHGLYLFDGMPLYEYKHEHDRENWEWGTANFDLGKREVHSFLISNALYWIEMYHIDGFRVDAVANLLYWPNR
PQPEANGFAIEFIQTLNEQVFAKDPHFLMIAEDSTDWPLVTHPTYEGGLGFNYKWNMGWMNDVLTYMEASPEQRKHLHHL
VSFSLMYAYSENYILPFSHDEVVYRKKSLLHKMPGDYWQKFAQYRLLISYFMMHPGKKLLFMGGEFAQFDEWKDEEELDW
FLDDFDMHRKARMFTKEALHLYKKSRILYENDHRQQGFEWIDVNNAEQSIVSFIRYGKQPGEALIIVCNFTPSVYHEYRV
GVPFQTEYIEVLNSDDAKYGGSHQINPKRLQARKGVLHGKPYSISMTVPPLGAAVFRAVKKRGAKKG
>Mature_626_residues
AGVSASDIKAHLFHEGRLYESYRFFGAHQTEQDGRQGFRFCVWAPHAKEVRIAGTFNGWSGVLHRMEKRHQEGIWELFIP
GIGEGELYKYEMITAANETKLKADPYAFFSEVRPKTASVTYQLAGYQWGDRKWQKKKQQKAVYEKPISIYELHIGSWKKK
ADGRFFTYRELSETVIPYVKEHGFTHIELMPLTEHPFDRSWGYQTTGYYSPTSRYGEPHDLMYFIDQCHQHEIGVIMDWV
SGHFCKDDHGLYLFDGMPLYEYKHEHDRENWEWGTANFDLGKREVHSFLISNALYWIEMYHIDGFRVDAVANLLYWPNRP
QPEANGFAIEFIQTLNEQVFAKDPHFLMIAEDSTDWPLVTHPTYEGGLGFNYKWNMGWMNDVLTYMEASPEQRKHLHHLV
SFSLMYAYSENYILPFSHDEVVYRKKSLLHKMPGDYWQKFAQYRLLISYFMMHPGKKLLFMGGEFAQFDEWKDEEELDWF
LDDFDMHRKARMFTKEALHLYKKSRILYENDHRQQGFEWIDVNNAEQSIVSFIRYGKQPGEALIIVCNFTPSVYHEYRVG
VPFQTEYIEVLNSDDAKYGGSHQINPKRLQARKGVLHGKPYSISMTVPPLGAAVFRAVKKRGAKKG

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=659, Percent_Identity=26.4036418816388, Blast_Score=176, Evalue=7e-44,
Organism=Escherichia coli, GI1789839, Length=625, Percent_Identity=43.84, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17554896, Length=612, Percent_Identity=25.1633986928105, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI32564391, Length=565, Percent_Identity=24.9557522123894, Blast_Score=151, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=633, Percent_Identity=25.2764612954186, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28573410, Length=643, Percent_Identity=27.3716951788491, Blast_Score=187, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_BACLD (Q65FS4)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080367.1
- RefSeq:   YP_092783.1
- ProteinModelPortal:   Q65FS4
- STRING:   Q65FS4
- EnsemblBacteria:   EBBACT00000057542
- EnsemblBacteria:   EBBACT00000061901
- GeneID:   3027988
- GeneID:   3099151
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03230
- KEGG:   bli:BL01419
- NMPDR:   fig|279010.5.peg.153
- eggNOG:   COG0296
- GeneTree:   EBGT00070000031950
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   BLIC279010-1:BLI03230-MONOMER
- BioCyc:   BLIC279010:BL01419-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 73820; Mature: 73689

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: ACT_SITE 204-204 ACT_SITE 239-239 ACT_SITE 244-244 ACT_SITE 307-307 ACT_SITE 309-309 ACT_SITE 352-352 ACT_SITE 419-419 ACT_SITE 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVSASDIKAHLFHEGRLYESYRFFGAHQTEQDGRQGFRFCVWAPHAKEVRIAGTFNGW
CCCCCCHHHHEEEEECCCEEEHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCHH
SGVLHRMEKRHQEGIWELFIPGIGEGELYKYEMITAANETKLKADPYAFFSEVRPKTASV
HHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCCCCEEE
TYQLAGYQWGDRKWQKKKQQKAVYEKPISIYELHIGSWKKKADGRFFTYRELSETVIPYV
EEEECCEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
KEHGFTHIELMPLTEHPFDRSWGYQTTGYYSPTSRYGEPHDLMYFIDQCHQHEIGVIMDW
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCEEEEC
VSGHFCKDDHGLYLFDGMPLYEYKHEHDRENWEWGTANFDLGKREVHSFLISNALYWIEM
CCCCEECCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEE
YHIDGFRVDAVANLLYWPNRPQPEANGFAIEFIQTLNEQVFAKDPHFLMIAEDSTDWPLV
EEECCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
THPTYEGGLGFNYKWNMGWMNDVLTYMEASPEQRKHLHHLVSFSLMYAYSENYILPFSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVYRKKSLLHKMPGDYWQKFAQYRLLISYFMMHPGKKLLFMGGEFAQFDEWKDEEELDW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCCHHHH
FLDDFDMHRKARMFTKEALHLYKKSRILYENDHRQQGFEWIDVNNAEQSIVSFIRYGKQP
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCHHHHHHHHHHCCCCC
GEALIIVCNFTPSVYHEYRVGVPFQTEYIEVLNSDDAKYGGSHQINPKRLQARKGVLHGK
CCEEEEEEECCHHHHHHEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCC
PYSISMTVPPLGAAVFRAVKKRGAKKG
CEEEEEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AGVSASDIKAHLFHEGRLYESYRFFGAHQTEQDGRQGFRFCVWAPHAKEVRIAGTFNGW
CCCCCHHHHEEEEECCCEEEHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCHH
SGVLHRMEKRHQEGIWELFIPGIGEGELYKYEMITAANETKLKADPYAFFSEVRPKTASV
HHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCCCCEEE
TYQLAGYQWGDRKWQKKKQQKAVYEKPISIYELHIGSWKKKADGRFFTYRELSETVIPYV
EEEECCEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
KEHGFTHIELMPLTEHPFDRSWGYQTTGYYSPTSRYGEPHDLMYFIDQCHQHEIGVIMDW
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCEEEEC
VSGHFCKDDHGLYLFDGMPLYEYKHEHDRENWEWGTANFDLGKREVHSFLISNALYWIEM
CCCCEECCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEE
YHIDGFRVDAVANLLYWPNRPQPEANGFAIEFIQTLNEQVFAKDPHFLMIAEDSTDWPLV
EEECCEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
THPTYEGGLGFNYKWNMGWMNDVLTYMEASPEQRKHLHHLVSFSLMYAYSENYILPFSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVYRKKSLLHKMPGDYWQKFAQYRLLISYFMMHPGKKLLFMGGEFAQFDEWKDEEELDW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCCHHHH
FLDDFDMHRKARMFTKEALHLYKKSRILYENDHRQQGFEWIDVNNAEQSIVSFIRYGKQP
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCHHHHHHHHHHCCCCC
GEALIIVCNFTPSVYHEYRVGVPFQTEYIEVLNSDDAKYGGSHQINPKRLQARKGVLHGK
CCEEEEEEECCHHHHHHEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCC
PYSISMTVPPLGAAVFRAVKKRGAKKG
CEEEEEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA