Click here to switch to the map view.

The map label for this gene is ytzG [H]

Identifier: 52786879

GI number: 52786879

Start: 3035464

End: 3036186

Strand: Reverse

Name: ytzG [H]

Synonym: BLi03154

Alternate gene names: 52786879

Gene position: 3036186-3035464 (Counterclockwise)

Preceding gene: 52786880

Following gene: 52786876

Centisome position: 71.9

GC content: 47.99

Gene sequence:

>723_bases
ATGAGATTGGATAAACTGTTGTCAAACAGCGGCTATGGTTCGAGAAAAGAAGTCAAAAAAATGCTGAAAAACGGCGCGGT
GCGCGTCAATGATCAATTAGTCAAAGACGCCAAAAAGCACGTTGACCCGGAATCAGACGACATCACGGTATACGGGGCAC
CCGTCATGTACCGGGAATTTATTTACTTAATGATGAACAAGCCGCAAGGAGTGCTGTCAGCAACAGAAGACAGCCGGCAG
GAAACGGTTGTCGATTTGCTCGACCCGGAGCTGCTGAGGTTTGAGCCGTTTCCTGTCGGAAGGCTTGACAAGGATACTGA
GGGACTGCTGCTTCTGACGAATGACGGACAGTTGGCACACCAGCTTTTATCTCCGAAAAAACACGTACCTAAAACGTATG
AAGTCCATGTCAATAAGCCGATTGATCAAGCGGCACTTGATCAGCTTGAAAAAGGGGTCGATATCGGTGAAGGATATGTC
ACGAAACCTGCTGAAGCATCGCTTCTGGCAAGCGAAAACGGCGTAGAACGCCTGCTTTTGACAATAACGGAAGGAAAATT
TCATCAGGTGAAAAGAATGCTGAAGGCGGTCGGCCATGACGTCATCTATTTAAAGCGGCTGAAAATGGGGGATGTCGATC
TCGATGAAGAGCTATTGCCAGGGGAATACAGGGAGCTCTCTGAACACGAGCTTGCCGCATTGCAAAACAAAAAGACACTC
TGA

Upstream 100 bases:

>100_bases
ACTGCTCGAAAAAATCTTCGGTCAGCGATTGAATCGCTTTTTCAAAAGAAAGGCCTCCTAAAAAGGGCTTTTCTTTTTAT
GTAAAAAAAGGGGTGTCTTC

Downstream 100 bases:

>100_bases
TTCATCGAGTGCCTGAAATGGGTTTGTGGAGCATCGAACTTAACCAAGTTTGATGCTTTTTATGTTTGATGGCTTTCTTA
TATGATATTCAGCGCTAAAG

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRLDKLLSNSGYGSRKEVKKMLKNGAVRVNDQLVKDAKKHVDPESDDITVYGAPVMYREFIYLMMNKPQGVLSATEDSRQ
ETVVDLLDPELLRFEPFPVGRLDKDTEGLLLLTNDGQLAHQLLSPKKHVPKTYEVHVNKPIDQAALDQLEKGVDIGEGYV
TKPAEASLLASENGVERLLLTITEGKFHQVKRMLKAVGHDVIYLKRLKMGDVDLDEELLPGEYRELSEHELAALQNKKTL

Sequences:

>Translated_240_residues
MRLDKLLSNSGYGSRKEVKKMLKNGAVRVNDQLVKDAKKHVDPESDDITVYGAPVMYREFIYLMMNKPQGVLSATEDSRQ
ETVVDLLDPELLRFEPFPVGRLDKDTEGLLLLTNDGQLAHQLLSPKKHVPKTYEVHVNKPIDQAALDQLEKGVDIGEGYV
TKPAEASLLASENGVERLLLTITEGKFHQVKRMLKAVGHDVIYLKRLKMGDVDLDEELLPGEYRELSEHELAALQNKKTL
>Mature_240_residues
MRLDKLLSNSGYGSRKEVKKMLKNGAVRVNDQLVKDAKKHVDPESDDITVYGAPVMYREFIYLMMNKPQGVLSATEDSRQ
ETVVDLLDPELLRFEPFPVGRLDKDTEGLLLLTNDGQLAHQLLSPKKHVPKTYEVHVNKPIDQAALDQLEKGVDIGEGYV
TKPAEASLLASENGVERLLLTITEGKFHQVKRMLKAVGHDVIYLKRLKMGDVDLDEELLPGEYRELSEHELAALQNKKTL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=38.5593220338983, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=31.6666666666667, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=35.1648351648352, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27043; Mature: 27043

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLSNSGYGSRKEVKKMLKNGAVRVNDQLVKDAKKHVDPESDDITVYGAPVMYREF
CCHHHHHCCCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
IYLMMNKPQGVLSATEDSRQETVVDLLDPELLRFEPFPVGRLDKDTEGLLLLTNDGQLAH
HHHHHCCCCCCEECCCHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCEEEEECCCHHHH
QLLSPKKHVPKTYEVHVNKPIDQAALDQLEKGVDIGEGYVTKPAEASLLASENGVERLLL
HHHCCHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEE
TITEGKFHQVKRMLKAVGHDVIYLKRLKMGDVDLDEELLPGEYRELSEHELAALQNKKTL
EECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLDKLLSNSGYGSRKEVKKMLKNGAVRVNDQLVKDAKKHVDPESDDITVYGAPVMYREF
CCHHHHHCCCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
IYLMMNKPQGVLSATEDSRQETVVDLLDPELLRFEPFPVGRLDKDTEGLLLLTNDGQLAH
HHHHHCCCCCCEECCCHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCEEEEECCCHHHH
QLLSPKKHVPKTYEVHVNKPIDQAALDQLEKGVDIGEGYVTKPAEASLLASENGVERLLL
HHHCCHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEE
TITEGKFHQVKRMLKAVGHDVIYLKRLKMGDVDLDEELLPGEYRELSEHELAALQNKKTL
EECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]