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The map label for this gene is ccpA [H]

Identifier: 52786850

GI number: 52786850

Start: 3007268

End: 3008269

Strand: Reverse

Name: ccpA [H]

Synonym: BLi03125

Alternate gene names: 52786850

Gene position: 3008269-3007268 (Counterclockwise)

Preceding gene: 52786851

Following gene: 52786849

Centisome position: 71.24

GC content: 47.8

Gene sequence:

>1002_bases
ATGAGTAATGTGACAATATATGATGTAGCACGCGAAGCAAATGTAAGTATGGCAACCGTTTCCCGGGTCGTCAACGGGAA
TCCGAATGTCAAGCCGACGACGAGAAAGAAGGTGCTTGAAGCGATCGAGCGTCTTGGCTATCGTCCAAACGCCGTGGCAA
GAGGGCTGGCAAGCAAAAAGACGACGACTGTCGGCGTGATCATTCCCGATATTTCCAGTATCTTTTATTCAGAGCTGGCG
AGGGGAATCGAAGATATCGCAACGATGTACAAGTACAATATTATTTTGAGCAATTCCGACCAGAATATGGACAAAGAACT
TCATCTTTTGAATACGATGCTAGGAAAGCAAGTTGACGGTATCGTCTTTATGAGCGGAAATGTGACTGAAGAGCATGTGG
AGGAATTTAAGCGATCGCCAGTTCCGATCGTGCTTGCGGCATCTGTTGAAGAAAAAGGGGAAACGCCGTCGGTTGCGATC
GATTATGAACAGGCGATTTATGATGCGGCAACCATGCTGATTGAGAAAGGCCATAAGCGCCTTGCGTTCGTCTCAGGACC
TATGGCTGAACCGGTTAATCAAGCGAAAAAACTGCAAGGCTTTAAAAGAGCGCTTGGGGATAAGGGGCTGCAATTTAAGG
AAGAGTATGTTGTAGAAGGCGATTATACGTACGATTCAGGAATGGAAGCGCTGGAGGCTTTAATGAAGCTGGATGAAAAA
CCGACAGCCGTCCTGTCAGCGACAGACGAAATGGCGCTCGGCGTTATTCACGCAGCACAGGATAAAGGACTGGCTGTTCC
GGATGACCTTGAAGTGATCGGTTTTGACAATACAAGGCTTTCATTAATGGTTCGGCCGCAGCTGTCGACTGTCGTCCAGC
CTACGTATGATATCGGTGCCGTGGCGATGAGGCTTCTGACAAAGCTGATGAACAAAGAGGAAGTCGAAGAGCATATTGTC
CAGCTGCCGCATCGCATTGAACTCAGACAATCAACAAAATGA

Upstream 100 bases:

>100_bases
TGTTTATTTTACATAAAGTTTTACATTTTGACATTTCTCTACATGAAAATGTTTATGCTATAGGAAAAGAAAAGTGTACC
CAGTTGAAGGAGTGGTAAAA

Downstream 100 bases:

>100_bases
TCATCATTAAATAAACGAGAAAGAAAGCAAGTGTTCACAGCTTTTTTCGTGAATTGCTTGCTTTCTTTTCCTTGAGCTGG
AGAGATTATGAAAAAATGAA

Product: hypothetical protein

Products: NA

Alternate protein names: Glucose-resistance amylase regulator [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSNVTIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELA
RGIEDIATMYKYNIILSNSDQNMDKELHLLNTMLGKQVDGIVFMSGNVTEEHVEEFKRSPVPIVLAASVEEKGETPSVAI
DYEQAIYDAATMLIEKGHKRLAFVSGPMAEPVNQAKKLQGFKRALGDKGLQFKEEYVVEGDYTYDSGMEALEALMKLDEK
PTAVLSATDEMALGVIHAAQDKGLAVPDDLEVIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEEVEEHIV
QLPHRIELRQSTK

Sequences:

>Translated_333_residues
MSNVTIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELA
RGIEDIATMYKYNIILSNSDQNMDKELHLLNTMLGKQVDGIVFMSGNVTEEHVEEFKRSPVPIVLAASVEEKGETPSVAI
DYEQAIYDAATMLIEKGHKRLAFVSGPMAEPVNQAKKLQGFKRALGDKGLQFKEEYVVEGDYTYDSGMEALEALMKLDEK
PTAVLSATDEMALGVIHAAQDKGLAVPDDLEVIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEEVEEHIV
QLPHRIELRQSTK
>Mature_332_residues
SNVTIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELAR
GIEDIATMYKYNIILSNSDQNMDKELHLLNTMLGKQVDGIVFMSGNVTEEHVEEFKRSPVPIVLAASVEEKGETPSVAID
YEQAIYDAATMLIEKGHKRLAFVSGPMAEPVNQAKKLQGFKRALGDKGLQFKEEYVVEGDYTYDSGMEALEALMKLDEKP
TAVLSATDEMALGVIHAAQDKGLAVPDDLEVIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEEVEEHIVQ
LPHRIELRQSTK

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (AmyE) and the acetyl-coenzyme A synthetase (AcsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=36.3344051446945, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=34.2424242424242, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=33.125, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=31.6666666666667, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=30.1324503311258, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=31.0240963855422, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=30.9278350515464, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=26.2295081967213, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=29.0419161676647, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=22.7692307692308, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787906, Length=252, Percent_Identity=26.5873015873016, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786268, Length=302, Percent_Identity=27.4834437086093, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=25.7142857142857, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790689, Length=332, Percent_Identity=24.6987951807229, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36785; Mature: 36654

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVTIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKK
CCCCEEEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCC
TTTVGVIIPDISSIFYSELARGIEDIATMYKYNIILSNSDQNMDKELHLLNTMLGKQVDG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC
IVFMSGNVTEEHVEEFKRSPVPIVLAASVEEKGETPSVAIDYEQAIYDAATMLIEKGHKR
EEEEECCCCHHHHHHHHCCCCCEEEEECCHHCCCCCCEEECHHHHHHHHHHHHHHCCCCE
LAFVSGPMAEPVNQAKKLQGFKRALGDKGLQFKEEYVVEGDYTYDSGMEALEALMKLDEK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHHCCCC
PTAVLSATDEMALGVIHAAQDKGLAVPDDLEVIGFDNTRLSLMVRPQLSTVVQPTYDIGA
CCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCHHHHH
VAMRLLTKLMNKEEVEEHIVQLPHRIELRQSTK
HHHHHHHHHHCHHHHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
SNVTIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKK
CCCEEEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCC
TTTVGVIIPDISSIFYSELARGIEDIATMYKYNIILSNSDQNMDKELHLLNTMLGKQVDG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC
IVFMSGNVTEEHVEEFKRSPVPIVLAASVEEKGETPSVAIDYEQAIYDAATMLIEKGHKR
EEEEECCCCHHHHHHHHCCCCCEEEEECCHHCCCCCCEEECHHHHHHHHHHHHHHCCCCE
LAFVSGPMAEPVNQAKKLQGFKRALGDKGLQFKEEYVVEGDYTYDSGMEALEALMKLDEK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHHCCCC
PTAVLSATDEMALGVIHAAQDKGLAVPDDLEVIGFDNTRLSLMVRPQLSTVVQPTYDIGA
CCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCHHHHH
VAMRLLTKLMNKEEVEEHIVQLPHRIELRQSTK
HHHHHHHHHHCHHHHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904524; 9387221; 9384377 [H]