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The map label for this gene is tyrS [H]

Identifier: 52786843

GI number: 52786843

Start: 2999677

End: 3000945

Strand: Reverse

Name: tyrS [H]

Synonym: BLi03118

Alternate gene names: 52786843

Gene position: 3000945-2999677 (Counterclockwise)

Preceding gene: 52786844

Following gene: 52786842

Centisome position: 71.07

GC content: 49.01

Gene sequence:

>1269_bases
ATGATGACACACATACTTGATGATTTGTCCTTCAGGGGACTGATTCAGCAGCAAACCGATGAAGCAGGCTTGAGAGAACT
GCTCGATAAAGAGAAAATCAGCTTATACTCCGGATTTGATCCGACAGCTGACAGTCTGCATATCGGCCACCTGCTGCCGA
TTTTAATGCTTCGCCGCTTCCAGCTGGCCGGACATCGTCCGATTGCGCTCGTAGGCGGGGCGACGGGCCTTATCGGCGAT
CCGAGCGGAAAGAAAGCGGAGCGCACGCTGAACAATGAAGAAATCGTCCGCGAATGGTCGCAGAAAATCAAAAACCAGCT
TTCGCGTTTCCTTGATTTTGAAGCTGAAGAGAACCCGGCGATCATGGCGAACAACTATGACTGGATCGGCAAAATGAACG
TCATTGATTTCCTGCGCGATGTCGGCAAAAACTTCGGAATCAATTACATGCTTGCCAAGGATACGGTAAGCTCCAGAATC
GAGACAGGCATATCTTATACTGAATTCAGCTACATGATCCTGCAGTCTCTCGATTTCTTGAATTTGTACAGAAACCAGGA
ATGCAAGCTTCAGATCGGCGGAAGCGACCAGTGGGGCAACATCACATCAGGTCTCGAACTCATCCGCAAATCCGAAGAGA
ATGCAAAAGCTTTCGGATTGACGATTCCGCTTGTGACAAAAGCAGACGGCACGAAGTTCGGAAAAACGGAAGGCGGCGCG
ATCTGGCTTGATAAAGAGAAAACGTCGGCGTATGAGTTTTATCAGTTCTGGATCAATACCGACGACAGGGATGTCGTGAA
ATACTTAAAATACTTCACTTTCTTATCAAAAGAAGAAATTGAAGATCTCGCTGAAAAAACAGAGACGGCGCCTGAAAAAC
GCGAAGCGCAAAAGCGCCTCGCCGAAGAAGTGACCGTTCTTGTACACGGCCGTGAAGCGTTTGAGCAGGCCGTCAACATC
TCAAAAGCACTGTTCAGCGGAGATATCAAACAATTGACAGCTGAAGAAGTAAAAGTCGGCTTTAAAGGGGTTCCTTCCCT
GGAGGTCGAGAAAGCGGATGAACTTCCTCTTGTTGAAATACTCGTCCAATCAAAGCTGTCTCCGTCTAAACGGCAGGCGC
GCGAAGATATCACGAATGGCGCGGTATACGTTAACGGCGAGCGCCGCACCGATGTTGCGGACGTCCTCACCGCCGACGAC
CGCATCGAAGGACAGTTTACCGTCATTCGCCGCGGTAAAAAGAAATACTTCCTGCTGACTTATAAGTAA

Upstream 100 bases:

>100_bases
GCGCGGCCATGCGTTCTTTTAAGCAGGGTGGTACCGCGATTTTTATCGTCCCTTCGTTTTTGCGAAGGGGCGTTTTATTT
TGGAAAAAAGGAGCTAATTG

Downstream 100 bases:

>100_bases
TCAAAAAAACTCTTGCCAAACGGCAAGGGTTTTTTTGTGTCAACATGTTTCATGTATGTTCATTCAGGAAATGTCAAAAA
TATAGATGTAATAGGTAGAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MMTHILDDLSFRGLIQQQTDEAGLRELLDKEKISLYSGFDPTADSLHIGHLLPILMLRRFQLAGHRPIALVGGATGLIGD
PSGKKAERTLNNEEIVREWSQKIKNQLSRFLDFEAEENPAIMANNYDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRI
ETGISYTEFSYMILQSLDFLNLYRNQECKLQIGGSDQWGNITSGLELIRKSEENAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSAYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEDLAEKTETAPEKREAQKRLAEEVTVLVHGREAFEQAVNI
SKALFSGDIKQLTAEEVKVGFKGVPSLEVEKADELPLVEILVQSKLSPSKRQAREDITNGAVYVNGERRTDVADVLTADD
RIEGQFTVIRRGKKKYFLLTYK

Sequences:

>Translated_422_residues
MMTHILDDLSFRGLIQQQTDEAGLRELLDKEKISLYSGFDPTADSLHIGHLLPILMLRRFQLAGHRPIALVGGATGLIGD
PSGKKAERTLNNEEIVREWSQKIKNQLSRFLDFEAEENPAIMANNYDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRI
ETGISYTEFSYMILQSLDFLNLYRNQECKLQIGGSDQWGNITSGLELIRKSEENAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSAYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEDLAEKTETAPEKREAQKRLAEEVTVLVHGREAFEQAVNI
SKALFSGDIKQLTAEEVKVGFKGVPSLEVEKADELPLVEILVQSKLSPSKRQAREDITNGAVYVNGERRTDVADVLTADD
RIEGQFTVIRRGKKKYFLLTYK
>Mature_422_residues
MMTHILDDLSFRGLIQQQTDEAGLRELLDKEKISLYSGFDPTADSLHIGHLLPILMLRRFQLAGHRPIALVGGATGLIGD
PSGKKAERTLNNEEIVREWSQKIKNQLSRFLDFEAEENPAIMANNYDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRI
ETGISYTEFSYMILQSLDFLNLYRNQECKLQIGGSDQWGNITSGLELIRKSEENAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSAYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEDLAEKTETAPEKREAQKRLAEEVTVLVHGREAFEQAVNI
SKALFSGDIKQLTAEEVKVGFKGVPSLEVEKADELPLVEILVQSKLSPSKRQAREDITNGAVYVNGERRTDVADVLTADD
RIEGQFTVIRRGKKKYFLLTYK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=432, Percent_Identity=37.2685185185185, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=54.4811320754717, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25145802, Length=396, Percent_Identity=36.8686868686869, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI25145805, Length=331, Percent_Identity=33.2326283987915, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=36.4864864864865, Blast_Score=276, Evalue=4e-75,
Organism=Drosophila melanogaster, GI20130383, Length=436, Percent_Identity=40.3669724770642, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47847; Mature: 47847

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTHILDDLSFRGLIQQQTDEAGLRELLDKEKISLYSGFDPTADSLHIGHLLPILMLRRF
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHH
QLAGHRPIALVGGATGLIGDPSGKKAERTLNNEEIVREWSQKIKNQLSRFLDFEAEENPA
HHCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IMANNYDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIETGISYTEFSYMILQSLDFL
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLYRNQECKLQIGGSDQWGNITSGLELIRKSEENAKAFGLTIPLVTKADGTKFGKTEGGA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSAYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEDLAEKTETAPEKREAQKRL
EEEECCCCHHEEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHH
AEEVTVLVHGREAFEQAVNISKALFSGDIKQLTAEEVKVGFKGVPSLEVEKADELPLVEI
HHHHEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LVQSKLSPSKRQAREDITNGAVYVNGERRTDVADVLTADDRIEGQFTVIRRGKKKYFLLT
HHHHCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEEECCCCEEEEEE
YK
EC
>Mature Secondary Structure
MMTHILDDLSFRGLIQQQTDEAGLRELLDKEKISLYSGFDPTADSLHIGHLLPILMLRRF
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHH
QLAGHRPIALVGGATGLIGDPSGKKAERTLNNEEIVREWSQKIKNQLSRFLDFEAEENPA
HHCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IMANNYDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIETGISYTEFSYMILQSLDFL
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLYRNQECKLQIGGSDQWGNITSGLELIRKSEENAKAFGLTIPLVTKADGTKFGKTEGGA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSAYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEDLAEKTETAPEKREAQKRL
EEEECCCCHHEEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHH
AEEVTVLVHGREAFEQAVNISKALFSGDIKQLTAEEVKVGFKGVPSLEVEKADELPLVEI
HHHHEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LVQSKLSPSKRQAREDITNGAVYVNGERRTDVADVLTADDRIEGQFTVIRRGKKKYFLLT
HHHHCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEEECCCCEEEEEE
YK
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA