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The map label for this gene is braB [H]

Identifier: 52786828

GI number: 52786828

Start: 2986955

End: 2988292

Strand: Direct

Name: braB [H]

Synonym: BLi03102

Alternate gene names: 52786828

Gene position: 2986955-2988292 (Clockwise)

Preceding gene: 52786820

Following gene: 52786829

Centisome position: 70.74

GC content: 47.83

Gene sequence:

>1338_bases
ATGAAAACTTCACTATCCGCTAAGGAAACGACGGCGATTGGGCTTATGCTGTTCGCCCTCTTTTTCGGAGCGGGAAATAT
GATTTTTCCTCCCCAGCTTGGTCAAGCTGCCGGAGAGAATATTTGGCCGGCTATCATCGGATTCTTGCTGACGGGCGTTG
GTCTTCCTCTGCTCGGCGTCATCGCGGTTGCTTTAAAAGGGAGTGCAAAAGGCCTAGCTGACAAAGCCCACCCGGTCTTC
GGCACGATATTAATCGTCAGCATTTATTTAACGATCGGACCTTTATTTGCCATTCCGAGGACGGGAAACGTTTCATACGT
CATAGCGGTTGAGCCGTTTTTAGGAAAACATTCGTCAAGTCTTTATCTGTTCATCTTTACCCTGATCTTTTTCGGGATCA
CTTATTTCTTGGCGTTAAACCCAAGCAAGCTTGTTGACCGCATCGGTAAAATTTTAACACCGATATTATTGACTGTAATC
GCAATCTTAGTCATCAAAGCGTTTATCACCCCGATGGGCAGCATCGGGTCTGTGACAGAGGCTTACCAATCCGCACCGCT
GTTTGTCGGATTTTTAGAAGGGTATAAAACGATGGATGCGATCGCTTCAATTGTTTTTGGAATTGTCGTCATTACGGCAA
TTAAAGACCGCGGAGTCACAAATCCAAAGTCAATCGCCGCTTCATGCATCAAAGCGGGGATAGTCGCTGCAGTCGGTCTC
GGACTCGTTTACGTTTCACTCGCTTATTTAGGGGCGACAAGCGTTGAAACCGTCGGTTCGCTCGACAACGGCGGCGAAAT
ATTGTCCAAAGCTTCCGCATATTTGTTCGGCTCGCTTGGAAATGCCGTGCTCGGCATCGCGATCTTGTTCGCCTGCCTGA
CGACAAGCGTAGGACTTGTTTCTTCTTGCGGAGAGTATTTTTCAAATCTGATTCCGAAGCTCTCTTACAAAGCAGTTGTC
ATCATTGTGACGCTTTTCAGCCTTGTGATTGCCAATTTCGGACTGACAGAAATCATCTCGTTTTCAGTACCGATTCTGTC
TGCGATTTATCCGCTTGCGATCGTCGTCGTTTTGCTCTCATTCATTGATAAGCTGTTTAATGAACGGCGCGAGGTCTATA
TCGGCACCCTTATCCCGACAGGAATTCTGAGCATCATCGACGGATTAAACGCGGCGAAAATCCCGCTGGGGCCGGTTAAC
GATATCTTAAAAGCGAACATCCCGCTGTTCAGCATGGGTGTCGGCTGGGTCGTTCCGGCTGTTATCGGCGCAGTGATCGG
TTACATTGTGACATTTTTTATCGCTTTGCCGGAGGACAAATTAAAGAACGTCAGCTGA

Upstream 100 bases:

>100_bases
TCTGATTTTTAAAATATAATAGTCTTTGGACTTTTGTCATATTATATCACGAAAATAAATTGACAGAAAATTTAAACAAA
ATGCCAGGAGGTTCTTTTTA

Downstream 100 bases:

>100_bases
CCTTTGCAAAAAACAGCTCTGTTTAAAACAGGGCTGTTTTTTTATCTTTCATTTCTTTAGACAAAAAACCCGGCACACAT
CTTTGGAGAGACCCAAATAA

Product: BraB

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKTSLSAKETTAIGLMLFALFFGAGNMIFPPQLGQAAGENIWPAIIGFLLTGVGLPLLGVIAVALKGSAKGLADKAHPVF
GTILIVSIYLTIGPLFAIPRTGNVSYVIAVEPFLGKHSSSLYLFIFTLIFFGITYFLALNPSKLVDRIGKILTPILLTVI
AILVIKAFITPMGSIGSVTEAYQSAPLFVGFLEGYKTMDAIASIVFGIVVITAIKDRGVTNPKSIAASCIKAGIVAAVGL
GLVYVSLAYLGATSVETVGSLDNGGEILSKASAYLFGSLGNAVLGIAILFACLTTSVGLVSSCGEYFSNLIPKLSYKAVV
IIVTLFSLVIANFGLTEIISFSVPILSAIYPLAIVVVLLSFIDKLFNERREVYIGTLIPTGILSIIDGLNAAKIPLGPVN
DILKANIPLFSMGVGWVVPAVIGAVIGYIVTFFIALPEDKLKNVS

Sequences:

>Translated_445_residues
MKTSLSAKETTAIGLMLFALFFGAGNMIFPPQLGQAAGENIWPAIIGFLLTGVGLPLLGVIAVALKGSAKGLADKAHPVF
GTILIVSIYLTIGPLFAIPRTGNVSYVIAVEPFLGKHSSSLYLFIFTLIFFGITYFLALNPSKLVDRIGKILTPILLTVI
AILVIKAFITPMGSIGSVTEAYQSAPLFVGFLEGYKTMDAIASIVFGIVVITAIKDRGVTNPKSIAASCIKAGIVAAVGL
GLVYVSLAYLGATSVETVGSLDNGGEILSKASAYLFGSLGNAVLGIAILFACLTTSVGLVSSCGEYFSNLIPKLSYKAVV
IIVTLFSLVIANFGLTEIISFSVPILSAIYPLAIVVVLLSFIDKLFNERREVYIGTLIPTGILSIIDGLNAAKIPLGPVN
DILKANIPLFSMGVGWVVPAVIGAVIGYIVTFFIALPEDKLKNVS
>Mature_445_residues
MKTSLSAKETTAIGLMLFALFFGAGNMIFPPQLGQAAGENIWPAIIGFLLTGVGLPLLGVIAVALKGSAKGLADKAHPVF
GTILIVSIYLTIGPLFAIPRTGNVSYVIAVEPFLGKHSSSLYLFIFTLIFFGITYFLALNPSKLVDRIGKILTPILLTVI
AILVIKAFITPMGSIGSVTEAYQSAPLFVGFLEGYKTMDAIASIVFGIVVITAIKDRGVTNPKSIAASCIKAGIVAAVGL
GLVYVSLAYLGATSVETVGSLDNGGEILSKASAYLFGSLGNAVLGIAILFACLTTSVGLVSSCGEYFSNLIPKLSYKAVV
IIVTLFSLVIANFGLTEIISFSVPILSAIYPLAIVVVLLSFIDKLFNERREVYIGTLIPTGILSIIDGLNAAKIPLGPVN
DILKANIPLFSMGVGWVVPAVIGAVIGYIVTFFIALPEDKLKNVS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=433, Percent_Identity=40.1847575057737, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46632; Mature: 46632

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]