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The map label for this gene is pelB [H]

Identifier: 52786782

GI number: 52786782

Start: 2936488

End: 2937972

Strand: Direct

Name: pelB [H]

Synonym: BLi03053

Alternate gene names: 52786782

Gene position: 2936488-2937972 (Clockwise)

Preceding gene: 52786772

Following gene: 52786793

Centisome position: 69.54

GC content: 46.87

Gene sequence:

>1485_bases
ATGAAACTGATCAAAAACGCATCTTTTATCATTTCTTTTTTGGCTGCGGCCGGCATTTATTTTTTATTAGGTACAGTCGC
TGCTTCTGCGGCAAACGAAGATTATCCGGAACAGATGATCAGGCTGGAAAGCTCATCAGGCTTAAATATTACGCCCGCCG
GGAATCAAGACAACGCACCGCTAACAGCAAAACAGACGAGCGGAGAAAAAGAAGAAAGATGGAGGCTTGATACGTCTGAC
GGCAAACAATTCAAAATCAGAAATATGGATAGCGGCAAAATTATCATCCCTGCCCATTACGCACTGTCAGACAATAATCC
GGCTGTGGTCTACTATGACAATTCACGGAAGGAAGAGTTGTGGAATATCATCGGGGCCGACAAAGACGGAAACGGAGATT
TTATCACGTATAAAATCGTCAGTGCACAAAACAGCAGCCTTGCACTGACACTGGACGGCAGCGGAGTGAAGCTTGCCAAA
TATACGGGCAGCTCCGTCCAAAAGTGGAAGCTTCCAAGCGACGGGCTCGAAGGTTTCGCAGGCTATGCAAGGGAAACGAA
CGGCAAGCAGAAAACAGGCACAACCGGCGGCCTGCTTGGAAAAGTCGTCTATGTCAATAATCTGGGCGAACTGAAGGCCA
ATATTGAAGATTCAACGCCGCGCACGATTGTCGTCTCCAGCAATATCGGCGCTTCAGCCAAAACGGTATTAACGGTGGGC
GCCAATAAAACAATCATCGGCTCGTATGAAAAACATAAGCTGAATAACATTTACTTTAAAACAAAAGCGGACTCTGGCAA
CGTTATTTTCAAAAATCTGGTCATTGCACATGATGCATCCATAAATGAAAACAATGACATCCCTGTTTACATTACCGATT
CGAGAAACTACTGGATTGACCATGTCACATTCCAAGGCCACAGCTATACGGCAAACGGCCACGATCTCGACAAGCTCTTA
TACGTCGGCGCTAAAGCCGATTACGTCACACTGTCGCACAGCACATTCACAGACCACAGGTACGGCCTGATTCTCGGCTG
GCCTCAAGATGACAAGCAATACCACAGTATATATAATGGCTATCCGCGGATGACAATCAGCCACAATCGCTTTGAGAATC
TCTATGTCAGGGCGCCCGGGTTGATGCGTTACGGCTATTATCACGTGAAAAGCAATTATATCAACAATTACCACCTTGGC
TTCACGATTACGACATTGGCGAAAATATATTCTGAAGCCAATTACTTCGGCACGGGCAATGAGAAAGGCATACTGGATGA
TTACGGAGACGGCGCGTTTAAAGATGTCGGGTCATATCCGGCGATAAAGGGGCAGAAATCGCCTGAGACAAGCTGGACAC
CTTCATCCAACTACAGCTATCGGACGATGAAGGCCGGCAATGCCAAAGCTTTTGCCAAACGGTACGCAGGTGCGCAGCGC
ACCGCTCTGTACTATGCGAATTACAGCCAGTTTAAAAAAGACTAA

Upstream 100 bases:

>100_bases
GGCCCTAAAGCATATATTTCAAATACACCTTTGGTGCTACAATTTGCAACAGCGCTAAATTTTCACCGACAGAAGGCAGT
GTCTTCAAGGAAGGACAACC

Downstream 100 bases:

>100_bases
TCAAAAAAGTGAGCGCGACATTGCGCTCACTTTTCAATATTTGACCATGTATCAAGCAGCTGATGAAGCTGCGCTTCTGT
ATCTTTCAGACTGCCGCTGT

Product: PelB

Products: NA

Alternate protein names: PNL [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKLIKNASFIISFLAAAGIYFLLGTVAASAANEDYPEQMIRLESSSGLNITPAGNQDNAPLTAKQTSGEKEERWRLDTSD
GKQFKIRNMDSGKIIIPAHYALSDNNPAVVYYDNSRKEELWNIIGADKDGNGDFITYKIVSAQNSSLALTLDGSGVKLAK
YTGSSVQKWKLPSDGLEGFAGYARETNGKQKTGTTGGLLGKVVYVNNLGELKANIEDSTPRTIVVSSNIGASAKTVLTVG
ANKTIIGSYEKHKLNNIYFKTKADSGNVIFKNLVIAHDASINENNDIPVYITDSRNYWIDHVTFQGHSYTANGHDLDKLL
YVGAKADYVTLSHSTFTDHRYGLILGWPQDDKQYHSIYNGYPRMTISHNRFENLYVRAPGLMRYGYYHVKSNYINNYHLG
FTITTLAKIYSEANYFGTGNEKGILDDYGDGAFKDVGSYPAIKGQKSPETSWTPSSNYSYRTMKAGNAKAFAKRYAGAQR
TALYYANYSQFKKD

Sequences:

>Translated_494_residues
MKLIKNASFIISFLAAAGIYFLLGTVAASAANEDYPEQMIRLESSSGLNITPAGNQDNAPLTAKQTSGEKEERWRLDTSD
GKQFKIRNMDSGKIIIPAHYALSDNNPAVVYYDNSRKEELWNIIGADKDGNGDFITYKIVSAQNSSLALTLDGSGVKLAK
YTGSSVQKWKLPSDGLEGFAGYARETNGKQKTGTTGGLLGKVVYVNNLGELKANIEDSTPRTIVVSSNIGASAKTVLTVG
ANKTIIGSYEKHKLNNIYFKTKADSGNVIFKNLVIAHDASINENNDIPVYITDSRNYWIDHVTFQGHSYTANGHDLDKLL
YVGAKADYVTLSHSTFTDHRYGLILGWPQDDKQYHSIYNGYPRMTISHNRFENLYVRAPGLMRYGYYHVKSNYINNYHLG
FTITTLAKIYSEANYFGTGNEKGILDDYGDGAFKDVGSYPAIKGQKSPETSWTPSSNYSYRTMKAGNAKAFAKRYAGAQR
TALYYANYSQFKKD
>Mature_494_residues
MKLIKNASFIISFLAAAGIYFLLGTVAASAANEDYPEQMIRLESSSGLNITPAGNQDNAPLTAKQTSGEKEERWRLDTSD
GKQFKIRNMDSGKIIIPAHYALSDNNPAVVYYDNSRKEELWNIIGADKDGNGDFITYKIVSAQNSSLALTLDGSGVKLAK
YTGSSVQKWKLPSDGLEGFAGYARETNGKQKTGTTGGLLGKVVYVNNLGELKANIEDSTPRTIVVSSNIGASAKTVLTVG
ANKTIIGSYEKHKLNNIYFKTKADSGNVIFKNLVIAHDASINENNDIPVYITDSRNYWIDHVTFQGHSYTANGHDLDKLL
YVGAKADYVTLSHSTFTDHRYGLILGWPQDDKQYHSIYNGYPRMTISHNRFENLYVRAPGLMRYGYYHVKSNYINNYHLG
FTITTLAKIYSEANYFGTGNEKGILDDYGDGAFKDVGSYPAIKGQKSPETSWTPSSNYSYRTMKAGNAKAFAKRYAGAQR
TALYYANYSQFKKD

Specific function: Catalyzes the depolymerization of pectins of methyl esterification degree from 13 to 75%, with an endo mode of action. Can not degrade polygalacturonate [H]

COG id: COG3866

COG function: function code G; Pectate lyase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002022
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00544 Pec_lyase_C [H]

EC number: =4.2.2.10 [H]

Molecular weight: Translated: 54694; Mature: 54694

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIKNASFIISFLAAAGIYFLLGTVAASAANEDYPEQMIRLESSSGLNITPAGNQDNAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCEEEECCCCCCCC
LTAKQTSGEKEERWRLDTSDGKQFKIRNMDSGKIIIPAHYALSDNNPAVVYYDNSRKEEL
EEEECCCCCCHHHEEEECCCCCEEEEEECCCCEEEEEEEEEECCCCCEEEEECCCCHHHH
WNIIGADKDGNGDFITYKIVSAQNSSLALTLDGSGVKLAKYTGSSVQKWKLPSDGLEGFA
HHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCHHHHH
GYARETNGKQKTGTTGGLLGKVVYVNNLGELKANIEDSTPRTIVVSSNIGASAKTVLTVG
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCEEEEEEC
ANKTIIGSYEKHKLNNIYFKTKADSGNVIFKNLVIAHDASINENNDIPVYITDSRNYWID
CCCEEECCHHHCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCEEEE
HVTFQGHSYTANGHDLDKLLYVGAKADYVTLSHSTFTDHRYGLILGWPQDDKQYHSIYNG
EEEECCCEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCC
YPRMTISHNRFENLYVRAPGLMRYGYYHVKSNYINNYHLGFTITTLAKIYSEANYFGTGN
CCCEEEECCCCCEEEEECCCEEEEEEEEEECCCCCEEEECEEHHHHHHHHHCCCCCCCCC
EKGILDDYGDGAFKDVGSYPAIKGQKSPETSWTPSSNYSYRTMKAGNAKAFAKRYAGAQR
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCE
TALYYANYSQFKKD
EEEEEECHHHHCCC
>Mature Secondary Structure
MKLIKNASFIISFLAAAGIYFLLGTVAASAANEDYPEQMIRLESSSGLNITPAGNQDNAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCEEEECCCCCCCC
LTAKQTSGEKEERWRLDTSDGKQFKIRNMDSGKIIIPAHYALSDNNPAVVYYDNSRKEEL
EEEECCCCCCHHHEEEECCCCCEEEEEECCCCEEEEEEEEEECCCCCEEEEECCCCHHHH
WNIIGADKDGNGDFITYKIVSAQNSSLALTLDGSGVKLAKYTGSSVQKWKLPSDGLEGFA
HHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCHHHHH
GYARETNGKQKTGTTGGLLGKVVYVNNLGELKANIEDSTPRTIVVSSNIGASAKTVLTVG
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCEEEEEEC
ANKTIIGSYEKHKLNNIYFKTKADSGNVIFKNLVIAHDASINENNDIPVYITDSRNYWID
CCCEEECCHHHCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCEEEE
HVTFQGHSYTANGHDLDKLLYVGAKADYVTLSHSTFTDHRYGLILGWPQDDKQYHSIYNG
EEEECCCEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCC
YPRMTISHNRFENLYVRAPGLMRYGYYHVKSNYINNYHLGFTITTLAKIYSEANYFGTGN
CCCEEEECCCCCEEEEECCCEEEEEEEEEECCCCCEEEECEEHHHHHHHHHCCCCCCCCC
EKGILDDYGDGAFKDVGSYPAIKGQKSPETSWTPSSNYSYRTMKAGNAKAFAKRYAGAQR
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCE
TALYYANYSQFKKD
EEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]