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The map label for this gene is grpE

Identifier: 52786475

GI number: 52786475

Start: 2636035

End: 2636619

Strand: Reverse

Name: grpE

Synonym: BLi02740

Alternate gene names: 52786475

Gene position: 2636619-2636035 (Counterclockwise)

Preceding gene: 52786476

Following gene: 52786474

Centisome position: 62.44

GC content: 45.47

Gene sequence:

>585_bases
ATGGCAGAAGAAAAACAAAACGAAGAGCTAAACGAACAAGAGGAACTGAACGAGACAGAAGCGGAGACAGCCGAAGCTGA
ACAAACGGCTGCTGAAGCTGATGCCCCGGCAGAAGAAACTCAAACAGAAATGCTTGAAAAACAACTAAAAGAACTTCAAG
AACGTCTTGAAGAAAAAGAAAATAAACTTTTACGTGTTCAGGCGGACTTTGAAAACTACAAAAGACGCGCACGCCTTGAT
CTCGAGGCTGCTGAAAAGTACCGCTCCCAGCGCATCATCAGCGATTTGCTGCCGGCGCTTGACAACTTTGAGCGCGCACT
TCAGATCGATCCTGACAATGAGCAGACGAAAAGCCTGCTTCAAGGAATGGAAATGGTGCACCGTCAAATTTTGGAGGCTC
TTAAAAATGAAGGAGTTGAACAGATTCCATCAGTCGGAGAGCAATTTGACCCGAACATGCACCAAGCTGTTATGCAGGTT
GAGGATGAAGCGTACGAATCAAACGCAGTCGTTGAGGAACTGCAAAAAGGCTATAAACTTAAGGACCGGGTGATCCGTCC
TTCTATGGTAAAAGTCAATCAATAA

Upstream 100 bases:

>100_bases
GACAAGTTTGTATGATGGGTAAGGCAGTACCTGAAAACAAACAGAAGGGAGGAATCCCTTCTTTATCATGAAACTCATCC
TTTAAGGGAGGTGAACACAG

Downstream 100 bases:

>100_bases
CTACATAGCAGGAGGTAAAAATCATGAGCAAAGTAATCGGAATCGACTTGGGAACAACGAACTCATGCGTTGCAGTGCTT
GAAGGCGGAGAGCCGAAAGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MAEEKQNEELNEQEELNETEAETAEAEQTAAEADAPAEETQTEMLEKQLKELQERLEEKENKLLRVQADFENYKRRARLD
LEAAEKYRSQRIISDLLPALDNFERALQIDPDNEQTKSLLQGMEMVHRQILEALKNEGVEQIPSVGEQFDPNMHQAVMQV
EDEAYESNAVVEELQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_194_residues
MAEEKQNEELNEQEELNETEAETAEAEQTAAEADAPAEETQTEMLEKQLKELQERLEEKENKLLRVQADFENYKRRARLD
LEAAEKYRSQRIISDLLPALDNFERALQIDPDNEQTKSLLQGMEMVHRQILEALKNEGVEQIPSVGEQFDPNMHQAVMQV
EDEAYESNAVVEELQKGYKLKDRVIRPSMVKVNQ
>Mature_193_residues
AEEKQNEELNEQEELNETEAETAEAEQTAAEADAPAEETQTEMLEKQLKELQERLEEKENKLLRVQADFENYKRRARLDL
EAAEKYRSQRIISDLLPALDNFERALQIDPDNEQTKSLLQGMEMVHRQILEALKNEGVEQIPSVGEQFDPNMHQAVMQVE
DEAYESNAVVEELQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=171, Percent_Identity=35.0877192982456, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1788967, Length=174, Percent_Identity=37.3563218390805, Blast_Score=110, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17552458, Length=199, Percent_Identity=26.6331658291457, Blast_Score=63, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=32.5, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653432, Length=184, Percent_Identity=33.695652173913, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BACLD (Q65H53)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079888.2
- RefSeq:   YP_092304.1
- ProteinModelPortal:   Q65H53
- SMR:   Q65H53
- STRING:   Q65H53
- EnsemblBacteria:   EBBACT00000057034
- EnsemblBacteria:   EBBACT00000060690
- GeneID:   3029072
- GeneID:   3097787
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02740
- KEGG:   bli:BL02095
- NMPDR:   fig|279010.5.peg.3011
- eggNOG:   COG0576
- GeneTree:   EBGT00050000001213
- HOGENOM:   HBG732630
- ProtClustDB:   PRK14140
- BioCyc:   BLIC279010-1:BLI02740-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22502; Mature: 22371

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKQNEELNEQEELNETEAETAEAEQTAAEADAPAEETQTEMLEKQLKELQERLEEKE
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKLLRVQADFENYKRRARLDLEAAEKYRSQRIISDLLPALDNFERALQIDPDNEQTKSLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QGMEMVHRQILEALKNEGVEQIPSVGEQFDPNMHQAVMQVEDEAYESNAVVEELQKGYKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KDRVIRPSMVKVNQ
HHHHCCCCHHCCCC
>Mature Secondary Structure 
AEEKQNEELNEQEELNETEAETAEAEQTAAEADAPAEETQTEMLEKQLKELQERLEEKE
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKLLRVQADFENYKRRARLDLEAAEKYRSQRIISDLLPALDNFERALQIDPDNEQTKSLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QGMEMVHRQILEALKNEGVEQIPSVGEQFDPNMHQAVMQVEDEAYESNAVVEELQKGYKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KDRVIRPSMVKVNQ
HHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA