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The map label for this gene is ispH

Identifier: 52786431

GI number: 52786431

Start: 2595425

End: 2596369

Strand: Direct

Name: ispH

Synonym: BLi02695

Alternate gene names: 52786431

Gene position: 2595425-2596369 (Clockwise)

Preceding gene: 52786430

Following gene: 52786468

Centisome position: 61.46

GC content: 48.47

Gene sequence:

>945_bases
ATGAACGTAATTAAAATATCGCCGCGCGGCTATTGCTACGGTGTCGTCGACGCCATGGTCATCGCAAAAAACGCGGCTCT
CGATAAAAGTCTGCCGAGACCTATTTATATTTTAGGAATGATCGTTCACAATAAACATGTCACAGACGCATTTGAAAAAG
AGGGCATTTATACGCTTGACGGCCCGAATAGGCTCGAGATTTTGAATCAGGTCGACAAAGGAACGGTGATCTTCACCGCT
CACGGCGTTTCGCCCGAAGTCCGCAAAATAGCTGAGGATAAAGGTCTTGTCGCGATTGACGCCACCTGCCCTGATGTGAC
AAAAACACACGATTTAATCCGGAAAATGAAAGCCGAAGGCTATCACGTCATCTACATCGGAAAAAAAGGACACCCGGAGC
CTGAAGGAGCGGTCGGCGTCGCCCCGGATATCGTTCATCTTGTTGAAACCGAAGAAGATGTGGAAAGGCTGGACATTGAA
GCGGAGAAACTGATTGTCACGAACCAAACGACGATGTCCCAATGGGATGTCCACGATATTATGGAAAAGGTAAAAGAAAA
ATATCCGGATGTTGAATTCCACCAGGAGATTTGCCTGGCCACCCAAGTGCGTCAGGAAGCGGTGTCCGAACAAGCGAAAA
AAGCCGATTTGACGATCGTTGTCGGCGATCCGAAAAGCAATAACTCAAACCGCCTCGCCCAGGTCTCCGAAGAAATCGCG
GGCACGAAAGCATACCGGATCGGTGATTTAAGCGAATTAAAGCTGGAATGGCTTGACGGCGTAGAAACCGTCGCTGTAAC
GGCCGGCGCATCGACACCGACCCCGATTACAAAGGAAGTCATCCGCTTTTTAGAGCAATTCGATCATGAAGATCCGTCCA
CATGGAAGATCAGTCACGAAGTACCGCTGCATAAAATCTTGCCTAAAGTAAAAGCAAAAACCTGA

Upstream 100 bases:

>100_bases
GGGATCAGATATAATGAGGTCTATAGAGACTGCAAAAATAAGCCGTTTTTTACGGCTTTTTATCATGCCCGGCTCTTACT
TCTTTATAGGAGGACTAAGA

Downstream 100 bases:

>100_bases
AGCGCTAATAGCTTCAGGTTTTGTTTTTTATACAAACGTAAACGGATTCGTATTCGTATCCGATGGAAAGACCGAGACAT
CAAATTTTTTCTCCCGGCAC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNVIKISPRGYCYGVVDAMVIAKNAALDKSLPRPIYILGMIVHNKHVTDAFEKEGIYTLDGPNRLEILNQVDKGTVIFTA
HGVSPEVRKIAEDKGLVAIDATCPDVTKTHDLIRKMKAEGYHVIYIGKKGHPEPEGAVGVAPDIVHLVETEEDVERLDIE
AEKLIVTNQTTMSQWDVHDIMEKVKEKYPDVEFHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLDGVETVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWKISHEVPLHKILPKVKAKT

Sequences:

>Translated_314_residues
MNVIKISPRGYCYGVVDAMVIAKNAALDKSLPRPIYILGMIVHNKHVTDAFEKEGIYTLDGPNRLEILNQVDKGTVIFTA
HGVSPEVRKIAEDKGLVAIDATCPDVTKTHDLIRKMKAEGYHVIYIGKKGHPEPEGAVGVAPDIVHLVETEEDVERLDIE
AEKLIVTNQTTMSQWDVHDIMEKVKEKYPDVEFHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLDGVETVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWKISHEVPLHKILPKVKAKT
>Mature_314_residues
MNVIKISPRGYCYGVVDAMVIAKNAALDKSLPRPIYILGMIVHNKHVTDAFEKEGIYTLDGPNRLEILNQVDKGTVIFTA
HGVSPEVRKIAEDKGLVAIDATCPDVTKTHDLIRKMKAEGYHVIYIGKKGHPEPEGAVGVAPDIVHLVETEEDVERLDIE
AEKLIVTNQTTMSQWDVHDIMEKVKEKYPDVEFHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLDGVETVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWKISHEVPLHKILPKVKAKT

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=293, Percent_Identity=36.8600682593857, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_BACLD (Q65H97)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079844.1
- RefSeq:   YP_092260.1
- STRING:   Q65H97
- EnsemblBacteria:   EBBACT00000056938
- EnsemblBacteria:   EBBACT00000061689
- GeneID:   3027337
- GeneID:   3097611
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02695
- KEGG:   bli:BL03721
- NMPDR:   fig|279010.5.peg.539
- eggNOG:   COG0761
- GeneTree:   EBGT00050000002965
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   BLIC279010-1:BLI02695-MONOMER
- BioCyc:   BLIC279010:BL03721-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34897; Mature: 34897

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIKISPRGYCYGVVDAMVIAKNAALDKSLPRPIYILGMIVHNKHVTDAFEKEGIYTLD
CCEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHCCEEEEC
GPNRLEILNQVDKGTVIFTAHGVSPEVRKIAEDKGLVAIDATCPDVTKTHDLIRKMKAEG
CCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCC
YHVIYIGKKGHPEPEGAVGVAPDIVHLVETEEDVERLDIEAEKLIVTNQTTMSQWDVHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
MEKVKEKYPDVEFHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
GTKAYRIGDLSELKLEWLDGVETVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWKISHE
CCCEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECC
VPLHKILPKVKAKT
CCHHHHHHHHCCCC
>Mature Secondary Structure
MNVIKISPRGYCYGVVDAMVIAKNAALDKSLPRPIYILGMIVHNKHVTDAFEKEGIYTLD
CCEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHCCEEEEC
GPNRLEILNQVDKGTVIFTAHGVSPEVRKIAEDKGLVAIDATCPDVTKTHDLIRKMKAEG
CCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCC
YHVIYIGKKGHPEPEGAVGVAPDIVHLVETEEDVERLDIEAEKLIVTNQTTMSQWDVHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
MEKVKEKYPDVEFHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
GTKAYRIGDLSELKLEWLDGVETVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWKISHE
CCCEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECC
VPLHKILPKVKAKT
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA