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The map label for this gene is yisY [H]

Identifier: 52785981

GI number: 52785981

Start: 2193424

End: 2194284

Strand: Reverse

Name: yisY [H]

Synonym: BLi02236

Alternate gene names: 52785981

Gene position: 2194284-2193424 (Counterclockwise)

Preceding gene: 52785985

Following gene: 52785980

Centisome position: 51.96

GC content: 47.85

Gene sequence:

>861_bases
ATGTTCAGCCCTCGGAGACATTCACCTATTTACATCTTGGGAGTATCTTATGGTAAAGACATGATCGGGGGCGACGGCAT
GGGATATTTTATCGAGGCAGAACCAAGCGTTAATCTGTACGTTGAAGACATCAATCCTCAAAGCGGCAAAGCCATTGTAT
TTTTGCACGGCTGGCCATTAAGCCATCTCCAGTTTGAATATCAATTTGACGATCTTTCGCGAAAGGGATACCGCTGTATC
GGCATTGACTGGCGTGGATTCGGGCGTTCGGATAAGCCGGCGACGGGCTATAATTACAACCGTTTGGCTGATGATATCCG
CGCTGTCGTTGAGGCGCTTCAATTAAACAACTTCACGCTTGCCGGTCATTCAACCGGCGGCGCCATAGCACTCCGCTATA
TGTCCCGATATAGGGGCGCTGGGGTCTCAAAGCTGGTTTTGATCGATGCGGCGGCGCCTGTGGGATTTACTGAAGAAACG
GCAGCCAAATTGCTTCAGCAAGCGTCTAACGATCGTCCCAAGATGATGCGCGAAGTGACCGACACGTTTTTCTTTCAATA
TATTACACAACCGTTCTCAGACTGTTTTTTTCAATTGGGATTGCAGGCGGCAGGCTGGTCGACAAAAGCGGTCATCGAAA
TGCTGAGAGATGAGAAACTGTATGATGATCCGGAAAAAGTCGCTGCTCCAGCCTTAATCATTCACGGTATTCAAGACAAG
GTGATTCCGTTTGCACAAGCAAAGGAGCTCAGCCGCAAAATCAGAAATTCATATCTTGTTCCGTTTCAATACAGCGGACA
CGGTCCATTCTGGGAGGAACGCAAAATGTTCAATCGGATTTTAACGCAATTTATCGGTTGA

Upstream 100 bases:

>100_bases
TGAGGACAAACGATATCGCCAGACTTCTCAGAAGTGCCATCATTGAACAACCCTCCGTTTCTTGAAATAGTTTAACCAGT
CACGCCTTTTCAAATTCTTC

Downstream 100 bases:

>100_bases
TTTTATATTGAGGAGCTGCCACATAATGTTCAAAAAGCAGAGAGGAATATTTTGGATAATCATGTTATGATGATAATCAC
CGAGACTGCTTCTGCACCCT

Product: YisY

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MFSPRRHSPIYILGVSYGKDMIGGDGMGYFIEAEPSVNLYVEDINPQSGKAIVFLHGWPLSHLQFEYQFDDLSRKGYRCI
GIDWRGFGRSDKPATGYNYNRLADDIRAVVEALQLNNFTLAGHSTGGAIALRYMSRYRGAGVSKLVLIDAAAPVGFTEET
AAKLLQQASNDRPKMMREVTDTFFFQYITQPFSDCFFQLGLQAAGWSTKAVIEMLRDEKLYDDPEKVAAPALIIHGIQDK
VIPFAQAKELSRKIRNSYLVPFQYSGHGPFWEERKMFNRILTQFIG

Sequences:

>Translated_286_residues
MFSPRRHSPIYILGVSYGKDMIGGDGMGYFIEAEPSVNLYVEDINPQSGKAIVFLHGWPLSHLQFEYQFDDLSRKGYRCI
GIDWRGFGRSDKPATGYNYNRLADDIRAVVEALQLNNFTLAGHSTGGAIALRYMSRYRGAGVSKLVLIDAAAPVGFTEET
AAKLLQQASNDRPKMMREVTDTFFFQYITQPFSDCFFQLGLQAAGWSTKAVIEMLRDEKLYDDPEKVAAPALIIHGIQDK
VIPFAQAKELSRKIRNSYLVPFQYSGHGPFWEERKMFNRILTQFIG
>Mature_286_residues
MFSPRRHSPIYILGVSYGKDMIGGDGMGYFIEAEPSVNLYVEDINPQSGKAIVFLHGWPLSHLQFEYQFDDLSRKGYRCI
GIDWRGFGRSDKPATGYNYNRLADDIRAVVEALQLNNFTLAGHSTGGAIALRYMSRYRGAGVSKLVLIDAAAPVGFTEET
AAKLLQQASNDRPKMMREVTDTFFFQYITQPFSDCFFQLGLQAAGWSTKAVIEMLRDEKLYDDPEKVAAPALIIHGIQDK
VIPFAQAKELSRKIRNSYLVPFQYSGHGPFWEERKMFNRILTQFIG

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=281, Percent_Identity=24.5551601423488, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562298, Length=121, Percent_Identity=31.404958677686, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554012, Length=107, Percent_Identity=32.7102803738318, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32350; Mature: 32350

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSPRRHSPIYILGVSYGKDMIGGDGMGYFIEAEPSVNLYVEDINPQSGKAIVFLHGWPL
CCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
SHLQFEYQFDDLSRKGYRCIGIDWRGFGRSDKPATGYNYNRLADDIRAVVEALQLNNFTL
CCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
AGHSTGGAIALRYMSRYRGAGVSKLVLIDAAAPVGFTEETAAKLLQQASNDRPKMMREVT
ECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
DTFFFQYITQPFSDCFFQLGLQAAGWSTKAVIEMLRDEKLYDDPEKVAAPALIIHGIQDK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHEEECCHHH
VIPFAQAKELSRKIRNSYLVPFQYSGHGPFWEERKMFNRILTQFIG
CCCHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSPRRHSPIYILGVSYGKDMIGGDGMGYFIEAEPSVNLYVEDINPQSGKAIVFLHGWPL
CCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
SHLQFEYQFDDLSRKGYRCIGIDWRGFGRSDKPATGYNYNRLADDIRAVVEALQLNNFTL
CCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
AGHSTGGAIALRYMSRYRGAGVSKLVLIDAAAPVGFTEETAAKLLQQASNDRPKMMREVT
ECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
DTFFFQYITQPFSDCFFQLGLQAAGWSTKAVIEMLRDEKLYDDPEKVAAPALIIHGIQDK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHEEECCHHH
VIPFAQAKELSRKIRNSYLVPFQYSGHGPFWEERKMFNRILTQFIG
CCCHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]